POLYMERASE CHAIN REACTION AMPLIFYING DNA What do you need to replicate DNA? umZT5z5R8.

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Presentation transcript:

POLYMERASE CHAIN REACTION AMPLIFYING DNA What do you need to replicate DNA? umZT5z5R8

polymerase-chain-reaction-PCR-3D-animation-with-no- audio.html

WHICH DNA SEQUENCE IS AMPLIFIED?

TANDEM REPEATS (VNTR)

TANDEM REPEAT GENES 5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’ 5’TTAACCTTAACCTTAACC 3’

DETERMINING YOUR ALLELES CREATING DNA FINGERPRINT USING GEL ELECTROPHORESIS

SAMPLE GEL Ladder Suspect 1 Suspect 2 Crime Scene 5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’ 5’TTAACCTTAACCTTAACC 3’

HOW UNIQUE ARE YOU?

RFLP RESTRICTION FRAGMENT LENGTH POLYMORPHISM

EXAMPLE USE TTCC RESTRICTION ENZYME DNA SAMPLE ONE: ACTGTTCCACACTTCCGCACATCTTAATTCC DNA SAMLE TWO: GTTCCATGCCGAGTACGTTCCATGCTA

I AM YOUR FATHER….WELL MAYBE