Log concentration Ct value miR-21 × miR-106b let-7a

cancer control cancer (n=34)control (n=15) 5.00E E E E E-1 0 Concentrations (amol/μl) 1.00E E E E-3 A: miR E E E E-1 Concentrations (amol/μl) 1.00E E E E-3 cancer (n=34)control (n=15) cancer control B: miR-106b 2.50E E E E E E-1 Concentrations (amol/μl) 1.00E E E E-3 cancer (n=34)control (n=15) cancer control C: let-7a

miR-21 Concentrations (amol/μl) 2.00E E E E E E-1 0 Pre-Op 1 month post - Op p=0.013 miR-106b Concentrations (amol/μl) 2.00E E E E E-1 0 p=0.022 Pre-Op 1 month post - Op

miR-106b Concentrations (amol/μl) 5.00E E E-3 cancer patient controls let-7a Concentrations (amol/μl) 2.00E E E-3 cancer patient controls miR-17-5p Concentrations (amol/μl) 1.00E E E E E-4 cancer patient controls miR-21 Concentrations (amol/μl) 3.00E E E-3 cancer patient controls miR-106a Concentrations (amol/μl) 1.00E E E E E-4 cancer patient controls

1-Specificity Sensitivity miR-106b AUC=

miR-106a/let-7a AUC=0.879 Sensitivity Specificity

Sensitivity miR-17-5p AUC= Specificity Sensitivity miR-21 AUC= Specificity Sensitivity miR-106a AUC= Specificity

Sensitivity miR-17-5p/let-7a AUC= Specificity Sensitivity miR-21/let-7a AUC= Specificity Sensitivity miR-106b/let-7a AUC= Specificity

miR-106blet-7a case case case case case case case case Rate of higher expression level in gastric cancer tissue 87.5%12.5% Rate of lower expression level in gastric cancer tissue 12.5%87.5% Table 1 Expression of mature miRNAs in gastric cancer tissue versus those in normal tissue