1 Fig. S1 HeLa cells ROS dye added after trypsinization ROS dye added before trypsinization Percentage of DCF fluorescence positive cells (H 2 O 2 production) Percentage of ethidium fluorescence positive cells (O 2.- production) Control No AA Control No GP Control No AA Control No GP 3.74% 45.60% 3.54% 41.22% 3.74% 43.34% 3.54% 47.21% 4.35% 2.01% 5.15% 3.00% 4.35% 33.84% 34.73% 5.15%

2 Fig. S2 a b U87 Cells Ethidium, control Ethidium, GP starvation DCF, control DCF, GP starvation U87 Cells Ethidium, control Ethidium, AA starvation DCF, control DCF, AA starvation

3 No GP No AA NH 4 Cl++++ Time1 h6 h24 h48 h U87 cells Fig. S3 LC3-I LC3-II β-Actin

% Cells with AVOs or GFP-LC3 dots No 3-MA Plus 3-MA 4 Fig. S4 No GP No AA AutophagyAVOsGFP-LC3 Time (h)72 h48 h HeLa cells * * * * * p < a

5 b (ii) Control No GP Wort Wort+No GP NameM1 (%) Key Control No AA Wort+No AA NameM1 (%) Key HeLa wt Fig. S4 HeLa wt * * * * * * p<0.04 HeLa SOD2 # # # # #p>0.05 (iii) (i)

6 Control siRNA beclin-1 siRNA atg-7 siRNA * * * * * * ** * p<0.01 compared to control siRNA No GP No AA AutophagyAVOsGFP-LC3 Time (h)72 h48 h HeLa cells Fig. S4 c

7 HeLa cellswtSOD2wtSOD2 LysateMitochondrialCytosolic Actin COX2 SOD2 Fig. S5

8 Fig. S6 R ME T FluorescenceEthidiumDCF Time6 h HeLa cells ** * * * * * p<0.05

9 Fig. S7 R ME T FluorescenceEthidiumDCF Time6 h HEK293cells a b R ME T FluorescenceEthidiumDCF Time24 h HEK293cells * * * * *p<0.05 * * * * *

% Cells with GFP-LC3 dots 10 Fig. S7 R ME T Time24 h HeLa cells * * * * * p<0.01 c d R T ME NH 4 Cl -+ HEK293 cells LC3-I LC3-II β-actin

11 Fig. S8 b 1 mM H 2 O 2 -+ NH 4 Cl+ Time24 h HEK293 cells a LC3-II β-Actin LC3-I Ethidium fluorescenceDCF fluorescence Counts FL3-H Counts FL1-H Control 1 mM H 2 O 2 Treatment Control HEK293 cells