Selected Experiments in Biochemistry BIOC4107 IUG, Spring 2014 TMZ IUG, Spring 2014 TMZ

Instructor Dr. Tarek M. Zaida Contact Office: Administration Building B304 Office Phone: 2697 (ext.) mobile: or Office Hours Sat & Mon: Sun, Tue, Wed: 10-11

Course Description This course is designed for the students with a biochemistry major, and is intended to focus on some carefully selected important protocols, and techniques used widely in biochemistry lab.

Course Objectives 1. This course aims to provide the students with an opportunity to develop their qualitative analytical skills. 2. It is expected that students upon completion of this course, will have a sound knowledge on advanced protocols applied widely in biochemistry. 3. Students will get adapted to team work. 4. Helping students developing their communication skills. 5. At the end of this course students will be familiar with “how” to prepare reports on lab activities. 6. Encouraging students to think critically and know how to solve problems.

Course Topics Protein Dialysis, Determination of protein pI, Protein salting out, Enzyme kinetics, Aerobic respiration, anaerobic respiration, Bradford test, Separation of blood serum lipids by means of Thin Layer Chromatography, Color Reactions of Plant Pigments, and SDS-PAGE.

Type of Course Teaching This is a practice course that requires: - short lectures, - in-lab practical experiments, and - Participation in different electronic learning tools.

Prerequisites Practical Analytical Chemistry CHEM2110 Biochemistry I BIOCA2301 Biochemistry II BIOCB2301 Practical Biochemistry I BIOCA2101 Practical Biochemistry II BIOCB2101

Textbook(s) A. Main textbook: Selected Experiments in Biochemistry (Prof. Ahmad Thabet 2010) B. Additional textbook Practical Medical Biochemistry (Tarek M. Zaida, 2011)

Course Timing ExperimentActivity 1 st Lab ActivityProtein DialysisReport 1 2 nd Lab ActivityDetermination of Protein pIReport 2 3 rd Lab ActivityProtein Salting outReport 3 4 th Lab ActivityEnzyme KineticsReport 4, Quiz 1 5 th Lab ActivityAerobic RespirationReport 5 6 th Lab ActivityAnaerobic RespirationReport 6 7 th Lab ActivityBradford TestReport 7, Quiz 2 8 th Lab ActivitySeparation of Blood Serum Lipids by TLCReport 8 9 th Lab ActivityColor Reactions of Plant PigmentsReport 9 10 th Lab ActivitySDS-PAGE part IReport 10, Quiz 3 11 th Lab ActivitySDS-PAGE part IIReport 11

Evaluation There will be different types of evaluation that will include: 1. Reading the lab manual before the lab activity day 2. Answering the pre-lab questions during lab session, 3. Quizzes (3) 4. Writing Lab reports 5. Participation in the actual lab work. 6. Participation in the discussion with classmates through the electronic learning tools.

Course Policy - Attending all lab sessions is a mandatory. - Active engagement in different elearning tools (online quizzes, wikis, forum, chat, electronic reports). - Zero tolerance for not following given instructions during lab sessions. - Wearing the lab coat as soon as you step in the lab. - Acting responsibly during the entire lab session. - Team work is a must, including communication with group partners. - Giving up reports from the previous lab session must be electronically only. - No make ups are allowed.

Lab Safety/Health  It is my job to keep my dear students safe, and protect them against any type of danger that might be anticipated during the lab sessions due to the different kinds of hazardous materials that might be necessary for some experiments.  However students are expected to comply completely with lab’s safety rules, and will be held responsible for their own safety once they don’t follow instructor’s rules.

Grading Reading the lab manual before the lab session and answering the pre-lab questions during lab session. 5 Quizzes (3)15 Lab reports (electronically submitted)10 Active engagement in the actual lab work20 Being active in different elearning tools10 Final exam40 Total Grade100

Following up with the Course - Students may reach the course materials through moodle system anytime. In case of anticipating any type of problems, student may contact me at my mobile or via anytime. How to use moodle system - Students may go through the moodle system using guide found on the website of electronic learning under educational resources for students. - Students may also ask for assistance from the electronic learning center found in the IT building I400, phone 1573.