The comparing of natural antibacterial substance on E. Coli Manhasset Science Research Jean Cheng Period 4

Figure 1: Antimicrobial drugs resistance pattern of urinary E. coli from Southern Brazil Need

Figure 2: Chemical formula for Penicillin mistry/wp- content/uploads/2008/04/peni cillin-core1.png Knowledge Base

Figure 2: Chemical formula for Penicillin mistry/wp- content/uploads/2008/04/peni cillin-core1.png Knowledge Base

Figure3: Penicillin attacking the peptidocglycan ve_cell_wall.svg/800px-Gram_negative_cell_wall.svg.png ve_cell_wall.svg/800px-Gram_negative_cell_wall.svg.png Knowledge Base (cont)

Figure3: Penicillin attacking the peptidocglycan ve_cell_wall.svg/800px-Gram_negative_cell_wall.svg.png ve_cell_wall.svg/800px-Gram_negative_cell_wall.svg.png

Figure 4: Tea tree’s terpene chemical formula ges/R67_IV-1_ _8959.jpg Figure 5: Pectin Chemical formula Knowledge Base (cont)

Figure 6: Aloe vera Knowledge Base (cont)

Figure 7: Citral

Figure 8:Chemical Change in Allicin subjected to different conditions. (Ariga, et al. 2008) 96DFD197.gif Literature Review

Figure 9: Antimicrobial activity of aloe vera gel (Alemdar, et al. 2009) Literature Review (cont)

Figure 10:Yuzu fruit seed oil on different microbial (Nakazono, et al. 2006) Literature Review (cont)

Figure 11:12 different essential oil on different species of bacteria. (Rusenova, et al. 2009) Literature Review (cont)

Find which natural substance is best in inhibiting E. coli. Null Hypothesis: No effect Alternative Hypothesis: Tea tree oil is the best antimicrobial substance against E. coli Purpose

The comparing of natural antibacterial substance on E. Coli Essential oil and antibiotic plant substance N=12 Garlic (allicin) N=3 Tea Tree oil N=3 Petri dish, E.coli, filter paper, glass spreader Disc diffusion assay method SPSS ANOVA Test Aloe (anthraquinones) N=3 Yuzu fruit oil N=3 Millimeter Methodology

Bacteria on agar Inhibited Zone(mm) Paper disk with substance being tested Figure 12: Disk Diffusion method Protocols

Figure 13: es/fig10_5.jpg es/fig10_5.jpg Protocols (cont)

Not expensive doesn’t require large amount of space Some materials found in lab or supermarket E. coli is needed as well as agar, aloe, tea tree, yuzu, and garlic Natural antiseptic doesn’t have negative effect Do-Ability

Budget

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