Fall 2009 Junior Colloquium Team Members (in alphabetical order): Elizabeth Chen Charles Chiang Elyse Geibel Steven Geng Stevephen Hung Kathy Jee Angela.

Slides:



Advertisements
Similar presentations
Blood Transfusions. By Hayley & Pete.. Blood Transfusions. Blood transfusion is the process of transferring blood or blood-based products from one person.
Advertisements

2. Cannabinoids are provided in vials containing the cannabinoids as a resin diluted in ethanol. These vials are stored at -20ºC. 3. In order to prepare.
Acid-Base Module: “Ur-ine” for a great time!
Determination of pH, Ca2+ and Mg2+ in Saliva
Cytokines in Asthma: Effects on Human Pulmonary Fibroblasts Shreya Lankala, Agostino Molteni, Betty Herndon UMKC School of Medicine Background & Rationale.
Electrolysis Amy Jewel, Rob Larkin and Todd Haurin “Water will be the coal of the future.” -Jules Verne, 1874.
Organ Printer By Michael DaSilva. What is the organ printer An organ printer incorporates 2 technologies, tissue engineering and a 3D printer. Instead.
A homo-dimer of annexin V protects against ischemia reperfusion injury in lung transplantation K Hashimoto, H Kim, H Oishi, M Chen, I Iskender, J Sakamoto,
Hemicellulose hydrogels
Course Content Introduction to the Research Process
Re-Examination of the Design of Early Clinical Trials for Molecularly Targeted Drugs Richard Simon, D.Sc. National Cancer Institute linus.nci.nih.gov/brb.
Biomedical research methods. What are biomedical research methods? An integrated approach using chemical, mathematical and computer simulations, in vitro.
Stem Cell Therapy in Dogs A New Treatment for a New Decade Presented by Vet-Stem
Stem Cell Research. What are the purposes of stem cells? Inject stem cells into damaged tissue areas and stem cells regenerate damaged areas Being proposed.
Building a Roadmap for Research IT John Brussolo Research IT Program Director September 6, 2012 © 2012 The Regents of the University of Michigan.
1 Autologous Liver Cell Transplantation A new approach to liver disease therapy Presentation at ESAO Conference, September 6 th 2007, Krems Prof. Toni.
Results: / In Vitro Biocompatibility of Iron Filled Carbon Nanotubes Taylor,
Medicines and drugs Option D Part 1.
The Scientific Method involves a series of steps that are used to investigate a natural occurrence.
Chapter 1: Research Methods
UNIT 5 SEMINAR.  According to your text, in an acute care setting, an electronic health record integrates electronic data from multiple clinical systems.
Enzyme Activity Lab 13 AP Biology
 C HAPTERS 14 & 15 Code Blue Health Science Edition 4.
Ex Vivo Lung Perfusion with Adenosine A2A Receptor Agonist Decreases Ischemia-Reperfusion Injury in Donation after Cardiac Death CE Wagner, NH Pope, EJ.
QUALITY ASSURANCE Quality Assurance of Reagents, Supplies, And Laboratory Water.
A Method for Preserving Hearts with Hydrogen Sulfide Spring 2009 Proposal Defense Team Members (in alphabetical order) Elizabeth Chen Charles Chiang Elyse.
Biomedical Research. What is Biomedical Research Biomedical research is the area of science devoted to the study of the processes of life; prevention.
20/03/2008 Dept. of Pharmaceutics 1. Use of BIOINFORMATICS in Pharmaciutics 2  Presented By  Shafnan Nazar  Hamid Nasir 
What is the Research Question? Teacher - Cindy Johnson School - Palacios High School Mentor – Dr. John Ford Department of Nuclear Engineering (Radiation.
Antioxidant Attenuation of Yeast Mutagenesis Peter Chekan Central Catholic High School Pittsburgh.
HONEY ANTIMICROBIAL EFFECTS Alicia Grabiec Freeport Senior High School Grade 10.
© 2004 by Thomson Delmar Learning, a part of the Thomson Corporation. Fundamentals of Pharmacology for Veterinary Technicians Chapter 3 Therapeutic Range.
Using Stem Cells to Treat Disease The first step: establish stem cell lines (large groups of the same cells) Next: turn on specific genes so that the stem.
Objectives (BPS chapter 9) Producing data: experiments  Experiments  How to experiment badly  Randomized comparative experiments  The logic of randomized.
Scientific Method Review Run this PPT show to help you review for your midterm.
Developing medicines for the future and why it is challenging Angela Milne.
Who we are What we do Where we are going How you can help.
Materials and Methods Phase 1 Evaluated acute effects on fasting blood glucose and on post-oral glucose Groups 1 and 3 received distilled water Groups.
The FDA: Basic Facts It takes 12 to 15 years to develop a single drug Only 1 in 10,000 potential medications makes it completely through the process Only.
Review of MOCT Questions
Tuning and Controlling the Release Profiles of Functional Biomolecules through Optimal Learning Jesse Goodman Summer 2014 McAlpine Group.
Hydrogen Sulfide as a Novel Cardiovascular Therapeutic Muhammad Mobeen Zafar 12-arid-1337.
Microencapsulation of Leydig cells Team: Bryan Baxter Tim Eng Joe Zechlinski April Zehm BME 400 October 14, 2005.
Assessment of Drug Release and Permeation across Skin.
Protein Enhanced Bone Recovery By Amanda Walker Working with Ashley Aston Weiner Mentored by Prasad Shastri.
Stock Solutions Different experiments require solutions of various different molarities. It would not be practical to have multiple bottles of each type.
Scaffold Degradation Part II Effect of Ionic Strength on Scaffold Biodegradability Jay Sehgal North Allegheny Intermediate High School.
A Nursing Supervisor’s Role Nicole Atkins, RN Nursing W SUNY Utica Elizabeth Rengal, RN -Preceptor.
By: Zaib Momin. Synthroid is used by many children and also adults whose thyroid gland isn’t supplying enough hormones to keep overall metabolism in working.
Doctor of Physical Therapy Writing and Using Objectives in Clinical Education Harriet Lewis, PT, MS Co Academic Coordinator of Clinical Education Assistant.
By: Kate Hotard Nicholls State University
Nanoparticles are particles between 1 and 100 nanometers in size. In nanotechnology, a particle is defined as a small object that behaves as a whole unit.
Broccoli sprouts: An exceptionally rich source of inducers of enzymes that protect against chemical carcinogens Broccoli sprouts: An exceptionally rich.
Team Organ Storage and Hibernation
Bench to Bedside: Current Challenges in TBI Research
Drug Dosage Forms. Development of Drug.
Guidance for review of studies involving HCT/Ps and IND Basics
Maryam Rahmati 1, Ali Samadikuchaksaraei 2,3,4, Masoud Mozafari 1,5,
Biotechnology Objectives: At the end of this lecture we will be able to identify and describe the uses of biotechnology in society.
EFFECT OF STORAGE TEMPERATURE ON THE STABILITY OF TOTAL PARENTERAL NUTRITION ADMIXTURES PREPARED FOR INFANTS Acta Poloniae Pharmaceutica n Drug Research,
Manufacturing system design (MSD)
Therapeutic delivery of hydrogen sulfide for salvage of ischemic skeletal muscle after the onset of critical ischemia  Peter W. Henderson, MD, Natalia.
Biomedical Research.
P07021: Instrument to Detect Thromboemboli
Firdaus | Sofia | Nurainiza | Hafizah
Co-Requisite – Content Standard SB3 Students will derive the relationship between single-celled and multi-celled organisms and the increasing complexity.
Volume 70, Issue 2, Pages (July 2006)
Volume 114, Issue 5, Pages (May 1998)
Therapeutic Angiogenesis in Critical Limb Ischemia via the Localized Delivery of bFGF from Ionic Hydrogels Hans Layman1, Toby Brooks1, Maria-Grazia Spiga3,
A. A. Islet isolations from human cadaveric pancreases stored for prolonged periods. Islet yield, purity, and viability was determined in pancreases (n.
Presentation transcript:

Fall 2009 Junior Colloquium Team Members (in alphabetical order): Elizabeth Chen Charles Chiang Elyse Geibel Steven Geng Stevephen Hung Kathy Jee Angela Lee Christine Lim Sara Moghaddam-Taaheri Adam Pampori Kathy Tang Jessie Tsai Diana Zhong Team Mentor: John P. Fisher

The Organ Shortage  There are over 100,000 people are on organ donor lists  Only 77 of these patients receive transplants daily  Hearts are limited to 4 hours in storage Preservation-related injury

Organ Storage Today  Static Cold Storage University of Wisconsin Solution No significant improvements in last two decades  Continuous Perfusion Organ Care System Effective, but extremely expensive

Overall Objective  Idea: To modify clinical cold storage procedures in place using hydrogen sulfide (H 2 S)  H 2 S is a compound thought to induce suspended animation and prolong organ storage  NaHS reacts with water to form H 2 S

Hydrogen Sulfide  H 2 S is depleted from solution H 2 S metabolized by tissues Since H 2 S is a gas, it escapes from solution quickly Limited protection time due to this depletion  As a result, need to control delivery of H 2 S treatment

Controlled Drug Delivery  Hydrogels – polymer networks Gelatin  Crosslinking  Size of microspheres

Hypothesis Gelatin microspheres can sustain H 2 S levels in the heart and induce protective effects

Objective I H 2 S & Cells  Objective: To determine if heart cells metabolize H 2 S and how they are affected by H 2 S  Methods: After incubation, aqueous H 2 S levels will be measured using a Zinc Acetate assay Other assays to determine cell viability

Objective I NaHS Dosage Test  Objective: What is the most effective concentration of NaHS for storage solutions?  Methods: 0 to 100µM NaHS in UW solution Sample at 2, 4, 6, 8 hours ○ Cellular viability assays ○ Functional evaluation

Objective II Sustaining Release of H 2 S  Objective: To determine if gelatin microspheres can release H 2 S in a controlled fashion  Method: Vary crosslinkage Detect change in release rate using zinc acetate assay

NaHS in UW solution Rat heart submerged in NaHS loaded UW solution UW solution Rat heart submerged in UW solution Objective III Effects of Sustained Release  Objective: To determine the effect of sustained H 2 S on heart storage  Methods: H 2 S microspheres added to UW solution Stored at 4ºC for eight hours Biopsy at 2, 4, 6, 8 hours ○ Cell viability assays ○ Functional evaluation NaHS in UW solution Rat heart submerged in NaHS loaded UW solution and injected with NaHS loaded microspheres NaHS loaded microsphere PBS loaded microspheres Rat heart submerged in NaHS loaded UW solution and injected with PBS loaded microspheres NaHS in UW solution

Current Progress  Funding  Review Paper  IACUC application has been submitted to UMD School of Medicine  Cell Culture Obtained H9c2 cells, a rat heart cell line Cultured these cells into a suitable population Will start experiments shortly.

Microsphere Fabrication  Fabricated microspheres by spraying the gelatin solution into an oil/water emulsion  Currently capable of making microspheres at 4±2µm Outliers greater than 10µm are excluded by means of a filter

Crosslinking  Crosslinking by adding gluteraldehyde to the gelatin solution Crosslinking amount will be controlled by altering the gluteraldehyde concentration  Once crosslinked, the microspheres are loaded with the NaHS solution

H 2 S Release  H 2 S release will be measured via a zinc acetate assay 50uL 50uL PBS Repeat for each time point

Initial loading concentration of 6.7M NaHS, 1M gluteraldehyde Net H 2 S Released Over Time

In Conclusion  We have: Started fabrication of microspheres ○ Achieved desired sizes ○ Currently running H 2 S release tests Cultured the desired cells Submitted the IACUC proposal  We will: Establish the relationship between crosslinking amount and release rate Perform H 2 S experiments on the cell cultures Perform H 2 S experiments on whole rat hearts

Team Dynamics  Big group:13 people on the team Split into subgroups for efficiency  Rotate facilitators during meetings to divide leadership  Be realistic Learn to accept setbacks

THE END  Any questions?

Global Hypothesis (Detailed)  Gelatin microspheres used to administer controlled delivery of H2S to the heart will induce protective effects and cause a state of hibernation in order to prolong viability of the heart and reduce ischemia- reperfusion injury in transplants