Isolation and characterization of a novel Dehalobacter species strain TCP1 that reductively dechlorinates 2,4,6- trichlorophenol Shanquan Wang, Weijie.

Slides:

Advertisements

Similar presentations

Figure S1. Effect of threshold distance on the number of OTUs. The grey box indicates maximal pyrosequencing and PCR-noise (Behnke et al., 2010; Quince.

Advertisements

Figure 1: a, Unrooted phylogenetic tree derived from full-length amino acid sequence alignments showing the relationship of G. sulfurreducens pilin domain.

Fig. S1 Fig. S1 Correlation between microarray and qRT-PCR expression analyses. The linear regression compares expression ratios of salinity to control.

Table S1 (Ho et al.) Table S1 Primers and their sequences used in this study. Name Sequence CDPK1-F1 5-CTACAGATCTATGGGGAATCAGTGCCA-3' CDPK1-R15'-TCATAGATCTCTAAACTCCATTTTCACAGAT-3'

Figure S1 Approximate positions of primers listed in Supplementary Table S1 for F3H (a), Tdic101 (b) and dTdic102 (c). Black arrow heads show primers and.

Cell number (% vehicle control) cisplatin concentration (µM)gefitinib concentration (µM) cell number expression level (fluorescence intensity)

A b c d e f A B C RDsèèuu.,.,.,., Microbial characterisation before and after the treatment in the well A Annalisa Balloi, Massimo Marzorati 1, Francesca.

1 Culture and identification of infectious agents, Lecture 25 Dr. Alvin Fox.

BIO 244 GENERAL MICROBIOLOGY

1 Culture and identification of infectious agents Dr. Abdullatif Neamatallah.

PCR - Polymerase Chain Reaction PCR is an in vitro technique for the amplification of a region of DNA which lies between two regions of known sequence.

Figure 1: Borrelia burgdorferi. Spontaneous Generation Controversy.

Microbial Diversity.

Methods in Microbial Ecology

A.Selvapandiyan LBPUA, OBRR, CBER, FDA, Bethesda, MD July Multiplex fluorescence-based PCR assay to detect pathogens in blood Multiplex fluorescence-based.

CULTURE INDEPENDENT ANALYSIS OF MICROBIAL COMMUNITIES IN SOIL

Lecture The Historical Roots of Microbiology

Haloferax sulfurifontis sp. nov., a Halophilic Archaeon Isolated from a Low Salt Sulfide-rich Spring K. N. Savage 1, M. S. Elshahed 1, A. Oren 2, L. R.

Introduction Nitrogen is one of the most essential nutrients for plant growth and development. However, plants are unable to use nitrogen in its natural.

Vesicle-Mediated Transfer of Antibiotic Resistance Between Klebsiella pneumoniae and Serratia marcescens Ondraya Espenshade Department of Biological Sciences,

22.1 Enrichment Isolation –The separation of individual organisms from the mixed community Enrichment Cultures –Select for desired organisms through manipulation.

Monitoring of bacteria with culture-independent techniques

-Know that we can manipulate genomes by inserting or deleting certain genes. -What about synthesizing an entirely novel genome using sequencing technology?

Background Gregory Fischer Julie Anderson Daniel Herman  Department of Biology  University of Wisconsin-Eau Claire Heterologous expression of MBP1 from.

Background on Microbial Fuel Cells A bio-electric system –Microbially maintained ion gradient fuels electron flow, generating electricity Two phases of.

Classification of a Novel Marine Bacterial Species: HTCC 2207

Supplementary Fig. S1. 16S RNA Neighbor-joining (NJ) tree of Brevibacterium metallicus sp. nov. NM2E3 T (in bold) and related species of genus Brevibacterium.

Diversity of Soil Microbes. Approaches for Assessing Diversity Microbial community Organism isolation Culture Nucleic acid extraction Molecular characterization.

A Vinyl Chloride-Dechlorinating Culture That Reduces Trichloroethene through Predominantly 1,1-Dichloroethene Jingjing Zhang, Andrew Joslyn, and Pei C.

You’re never too old to streak!

Sources of Bacterial Species with Antibiotic Activity: Soil & Rotten Wood.

Overview Wednesday Thursday Labs 12, 13 & 14 due March 7th

American Society of Microbiology North Central Branch Meeting

Microbial ecology techniques

Identification of nifH and nodC genes from Rhizobium aegyptiacum

Gilda Carvalho, Cláudia Galinha, Teresa Crespo and Maria Reis

Copyright © 2017 American Academy of Pediatrics.

Workshop on the analysis of microbial sequence data using ARB

Molecular Phylogenetics

Supplemental material for Wang et al

RESULTS AND DISCUSSION

Experimental design for high‐throughput characterization of synthetic human gut microbiome consortia Experimental design for high‐throughput characterization.

Daniel A. Peterson, Daniel N. Frank, Norman R. Pace, Jeffrey I. Gordon

Taxonomical classification is recognizing and registering the worlds organism diversity – continual changing knowledge about evolutionary and ecological.

A Novel Streptococcus Organism Identified in a Case of Fulminant Endocarditis Using 16S rDNA Sequencing Zsolt Jobbagy, Claire B. Fabian, Vincent A. Memoli,

Evaluation of antimicrobial properties and their substances against pathogenic bacteria in-vitro by probiotic Lactobacilli strains isolated from commercial.

Fractions of 16S rRNA genes from bacteria (top panel) and archaea (bottom panel) in public databases from primer-amplified metagenomes (with and without.

Characterization of an Avian Influenza A (H5N1) Virus Isolated from a Child with a Fatal Respiratory Illness by Kanta Subbarao, Alexander Klimov, Jacqueline.

Phylogenetic tree of perA A2-domain DNA sequence.

Phylogenetic tree of 38 Pseudomonas type strains, based on the V3-V5 region sequence of the 16S rRNA gene (V3 primer, positions 442 to 492; and V5 primer,

Species diversity within the P. fluorescens species complex.

Proportion of 16S rRNA gene sequences in each category of phylogenetic novelty relative to cultures for each environment, by amplicons, metagenomes (without.

Daniel A. Peterson, Daniel N. Frank, Norman R. Pace, Jeffrey I. Gordon

K. S. Ko, T. Kuwahara, L. Haehwa, Y. -J. Yoon, B. -J. Kim, K. -H

Production of Mapk14 and Mapk7 KO cells.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

(a) Mycetoma aspect of the patient in the right forearm.

(A) The result of a qPCR assay comparing fluorescence with cycle number. (A) The result of a qPCR assay comparing fluorescence with cycle number. The results.

Sequence variation of 16S rRNA gene primer-binding sites.

Phylogenetic network with concatenated 16S rRNA, 23S rRNA, groEL, rpoB, and dnaK sequences (3,009 unambiguously aligned base pairs), including 71 Coxiella-like.

ITS rRNA gene locus. ITS rRNA gene locus. Schematic of the eukaryotic ribosomal gene cluster. The SILVA database contains sequences of the 18S gene, while.

Cell Culture Engineering XI

Multiplex PCR assay to detect common resistance determinants in B

Representative results showing PCR amplification of DNA from stools using PCR primers amplifying (A) SFB sequences and expected to generate an SFB 139 bp.

Phylogenetic tree based on predominant 16S rRNA gene sequences obtained by C4–V8 Sutterella PCR from AUT-GI patients, Sutterella species isolates, and.

Volume 24, Issue 5, Pages (March 2013)

DGGE analysis of tet(M) in water samples and in bacterial isolates.

Tree depicting the phylogenetic relationships of all strains included in this study. Tree depicting the phylogenetic relationships of all strains included.

(A and B) Maximum-likelihood trees of 28 strains of Pantoea agglomerans and closely related species, constructed using concatenated sequences of six protein-coding.

Addressing Antibiotic Resistance by Isolation and Characterization of Genus Lysobacter and Genus Unknown Antibiotic-producers in Pittsburgh Soil Miriam.

Presentation transcript:

Isolation and characterization of a novel Dehalobacter species strain TCP1 that reductively dechlorinates 2,4,6- trichlorophenol Shanquan Wang, Weijie Zhang, Kun-Lin Yang, and Jianzhong He*, Department of Civil and Environmental Engineering Department of Chemical and Biomolecular Engineering National University of Singapore, Singapore

Figure S1. DGGE characterization of dechlorinating bacteria present in culture TCP1 after 21 times of serial dilution. The PCR products were obtained by using the universal bacterial primer set, 341FGC and 518R. Dehalobacter 16S rRNA

5 µm Figure S2. Morphology of Dehalobacter sp. strain TCP1 as observed with fluorescence microscopy. Cells were stained with fluorescent dye Syto-9 (5 µM).

Dehalobacter sp. TCP1 1 Dehalobacter sp. AD14-PCE 2 Figure S3. RpoB DGGE characterization of Dehalobacter bacteria present in culture TCP1 (lane 1) and culture AD14-PCE (lane 2) with Dehalobacter genus-specific rpoB gene targeted primers, DebrpoBFGC and DebrpoBR.

Figure S4. Sequence heterogeneity of the five 16S rRNA genes in strain TCP1 when excluding the insertion sequences (from base ). 16S-1 (JX999720) 16S-2 (JX999721) 16S-3 (JX999722) 16S-4 (JX999723) 16S-5 (JX999724) 16S-1 (JX999720) 16S-2 (JX999721) 16S-3 (JX999722) 16S-4 (JX999723) 16S-5 (JX999724)

Figure S5. Phylogenetic tree of identified putative chlorophenol reductive dehalogenase gene - debcprA. Phylogenetic analyses are conducted in MEGA4 (Tamura et al. 2007). debcprA (Dehalobacter sp. TCP1, JX999725) putative rdhA (Dehalobacter sp. DCA, ) cprA (Desulfitobacterium chlororespirans, AAL84925) pceA (Desulfitobacterium hafniense Y51, YP519072) cprA (Desulfitobacterium sp. PCE-1, AF259790) cprA (Desulfitobacterium hafniense DCB-2, AY013365) cprA (Desulfitobacterium hafniense Y51, YP517388)