0 5 10 15 20 25 Wild type 0 minutes 10 minutes 30 minutes 0 5 10 15 20 0 5 10 15 20 25 0 5 10 15 20 ncsA Afu8g05010 (transcription factor) Fold increase.

Slides:

Advertisements

Similar presentations

Chapter 6 Elasticity and Demand.

Advertisements

Supplementary Figure 1 >15 alleles Sens: 44% 1-Spec: 19% alleles Sens: 54% 1-Spec: 66%

Supplementary Table 1. Immunological Characteristics of Patients.

WT GRX-V GRX-VI GRX-VII 12-day-old seedlings on MS medium20-day-old seedlings on soilrite Mature plants in pots Supplementary Fig. S1 Phenotype of wild-type.

WHAT DO THEY ALL MEAN?. Median Is the number that is in the middle of a set of numbers. (If two numbers make up the middle of a set of numbers then the.

Sl No Top-up Amount No Of Affiliate Ads Payment Per Day By Affiliate Ad Total Affiliate Ad Income 1.5,000/- Daily 2 ad for 100 days 100/- Affiliate.

Demand and Supply: TV Set (Australia)

Money Math Review.

Counting coins Teacher’s Notes Slides used as worksheet print outs rather than class presentation. Variations to quantity and appearance can be made easily.

Comparison of X-ray diffraction patterns of La 2 CuO 4+   from different crystals at room temperature Pia Jensen.

Number bonds to 10,

Let’s take a 15 minute break Please be back on time.

Bell Schedules Club Time is available from 8:05-8:20  1 st 8:20 – 9:15  2 nd 9:20 – 10:10  3 rd 10:15 – 11:05  4 th 11:10 – 12:50 A(11:10)

4.6 Quick Graphs Using Slope-Intercept Form 1 GOAL

Powerpoint Jeopardy Category 1Category 2Category 3Category 4Category

20,000 GENES IN HUMAN GENOME; WHAT WOULD HAPPEN IF ALL THESE GENES WERE EXPRESSED IN EVERY CELL IN YOUR BODY? WHAT WOULD HAPPEN IF THEY WERE EXPRESSED.

RNA Lab (Isolation, quantification and qPCR analysis) MCB7300.

RNA Electrophoresis. Broad and Long Term Objective To characterize the expression of ribulose 1-5 bisphosphate carboxylase oxygenase and chlorophyll AB.

How do you identify and clone a gene of interest? Shotgun approach? Is there a better way?

Supplementary Figure 1. Compararison of the expression of the genes within the 10q23 locus using either Troglitazone or Rosiglitazone as the PPAR  agonist.

RT-PCR analysis 생화학 실험 2 조교 : 이 선 민 내선 7699, 첨단과학기술관 201-B 호 신과학원 S438 호.

Volume 17, Issue 17, Pages (September 2007)

Figure S1 P. stipitis TRC1 N. crassa PDR-1 A. niger RhaR

Supplementary Figure S4

Volume 61, Issue 1, Pages (January 2002)

Supplemental Figure 2. (A) AtplaIVA-1 and AtplaIVA-2 null transcription lines for AtPLAIVA mRNA. RNAs from the relevant wild type Col were isolated.

Figure 3. Kinetics effects of the C domain on ER–EREc binding

a b c ** ** ** ** * * * P450arom mRNA Trascript Levels (Fold)

(x 10-3) (x 10-4) GST P WT mock WT #15 WT mock WT #15

Volume 61, Issue 1, Pages (January 2002)

Novel Molecular Method for Detection of Bovine-Specific Central Nervous System Tissues as Bovine Spongiform Encephalopathy Risk Material in Meat and Meat.

a b c Fold Change DeltaCt Cell Line ADAR1 ADAR2 Ratio H ,776

Supplementary Figure 4 a b c d e f

SAGA Is a General Cofactor for RNA Polymerase II Transcription

M.H.A. Baccora, P. Cortes, C. Hassett, D.W. Taube, J. Yee

Jin Yang, Hal P. Bogerd, P.Jeremy Wang, David C. Page, Bryan R. Cullen

Robyn P. Hickerson, Sancy A. Leachman, Lana N

Volume 135, Issue 2, Pages (August 2008)

Volume 71, Issue 9, Pages (May 2007)

SUMO Promotes HDAC-Mediated Transcriptional Repression

Enhancing and maintaining chondrogenesis of synovial fibroblasts by cartilage extracellular matrix protein matrilins M. Pei, M.D., Ph.D., J. Luo, M.D.,

Shinya Takahata, Yaxin Yu, David J. Stillman Molecular Cell

mRNA level as % relative to

Transcriptional Regulation of ATP2C1 Gene by Sp1 and YY1 and Reduced Function of its Promoter in Hailey–Hailey Disease Keratinocytes Hiroshi Kawada,

Marc Spingola, Manuel Ares Molecular Cell

Bernard et al, Supplementary Figure S1

Volume 37, Issue 6, Pages (March 2010)

Molecular Therapy - Nucleic Acids

Andrew W Snowden, Philip D Gregory, Casey C Case, Carl O Pabo

Volume 5, Issue 3, Pages (May 2012)

ADAR Regulates RNA Editing, Transcript Stability, and Gene Expression

UVB and Proinflammatory Cytokines Synergistically Activate TNF-α Production in Keratinocytes through Enhanced Gene Transcription Muhammad M. Bashir,

Characterization of Keratinocyte Differentiation Induced by Ascorbic Acid: Protein Kinase C Involvement and Vitamin C Homeostasis1 Isabella Savini, Antonello.

Dimethylation of H3K4 by Set1 Recruits the Set3 Histone Deacetylase Complex to 5′ Transcribed Regions TaeSoo Kim, Stephen Buratowski Cell Volume 137,

Stress-Induced Phosphorylation of S

Volume 58, Issue 5, Pages (June 2015)

Ying Tan, Zdravko Dragovic, Till Roenneberg, Martha Merrow

Volume 17, Issue 1, Pages (January 2007)

Supplementary Figure 1 A B C SW620 HT29 SW620

Volume 70, Issue 5, Pages (September 2006)

Quantitation of Hsp-72 mRNA in human muscle biopsies and heat-shocked NIH-3T3 cells, using competitive RT-PCR. Quantitation of Hsp-72 mRNA in human muscle.

Feng Xu, Qiongyi Zhang, Kangling Zhang, Wei Xie, Michael Grunstein

7 WT 6 irx1-6 ixr fold increased expression 3 2 n- 1 AT1G18710

Nucleoid Proteins Stimulate Stringently Controlled Bacterial Promoters

Preferential response of cancer cells to zebularine

Nicole Robbins, Michelle D. Leach, Leah E. Cowen Cell Reports

Deletion of VdHapX renders Verticillium dahliae more susceptible to H2O2. Deletion of VdHapX renders Verticillium dahliae more susceptible to H2O2. (A)

* Supplementary Fig. 1 (Yamanaka et al.) * * * ** ** ** * * **

Chih-Yung S. Lee, Tzu-Lan Yeh, Bridget T. Hughes, Peter J. Espenshade

Presentation transcript:

Wild type 0 minutes 10 minutes 30 minutes ncsA Afu8g05010 (transcription factor) Fold increase Wild type ncsA Afu1g17460 (transcription factor) Fold increase Afu2g16520 (phospholipase D) Fold increase Wild type ncsA Afu2g07630 (H + /Ca 2+ exchanger ) Wild type ncsA Fold increase A.B. C.D.

Afu2g13060 (calcineurin binding protein) Wild type ncsA Fold increase Afu2g11460 (transcription factor) Wild type ncsA Fold increase Afu3g05760 (transcription factor) Wild type ncsA Fold increase Afu4g03460 (transcription factor) Wild type ncsA Fold increase E.F. G.H.

Supplementary Figure 3 - Fold increase in mRNA levels after the incubation with 200 mM CaCl 2 for 10 and 30 minutes. Real-time RT-PCR was the method used to quantify the mRNA. The measured quantity of the (A) Afu8g05010 (C2H2 finger domain protein), (B) Afu1g17460 (C6 transcription factor), (C) Afu2g16520 (phospholipase D), (D) Afu2g07630 (vacuolar H + /Ca +2 exchanger), (E) Afu2g13060 (calcineurin binding protein), (F) Afu2g11460 (C6 finger domain protein), (G) Afu3g05760 (C6 transcription factor), and (H) Afu4g03460 (HLH DNA binding domain protein) mRNA in each of the treated samples was normalized using the C T values obtained for the -tubulin (Afu1g10910) mRNA amplifications run in the same plate. The relative quantitation of all the genes and tubulin gene expression was determined by a standard curve (i.e., C t –values plotted against logarithm of the DNA copy number). The results are the means standard deviation of four sets of experiments. The values represent the number of times the genes are expressed compared to the corresponding control strain (either the wild type or ncsA) grown before adding 200 mM CaCl 2 (represented absolutely as 1.00).