Microbiology

 Neutrophils: Phagocytic  Basophils: Produce histamine  Eosinophils: Toxic to parasites, some phagocytosis  Dendritic cells: Initiate adaptive immune response, phagocytic

 Monocytes: Phagocytic as mature macrophages  Fixed macrophages in lungs, liver, bronchi  Wandering macrophages roam tissues  Lymphocytes: Involved in specific immunity

 pseudopods of macrophage entrap E.coli bacteria  bacteria brought into macrophage for destruction

 Serum proteins activated in a cascade.  Activated via one of three pathways  Activated Complement proteins trigger a number of immune responses

 Opsonization  Cytolysis- membrane attack complex  Inflammation Figure 16.10

The complement system is an important part of the innate immune system that has far-reaching effects on the body’s ability to fight off infection. Many pathogens have the ability to affect or disable individual proteins within the Complement system. In your opinion, which Complement protein (C5a? C1?) would be most devastating to the host immune system if it were to be inactivated by a particular pathogen? Why?

 Bone marrow gives rise to B cells.  B cells have surface antibodies (Igs) that recognize epitopes  T-dependent  T-independent

Figure 17.9

 Specialized lymphocytes, mostly T cells, respond to intracellular Ags  After differentiating in the thymus, T cells migrate to lymphoid tissue  T cells differentiate into effector T cells when stimulated by an Ag  Some effector T cells become memory cells

 Helper T Cells (CD4, T H )  T H 1Activate cells related to cell-mediated immunity  T H 2Activate B cells to produce eosinophils, IgM, and IgE  Cytotoxic T Cells (CD8, T C )  Destroy target cells with perforin

As you have learned in this unit, the immune system does an exceptional job in clearing infections from the host. However, on occasion the immune system can actually make an illness worse by overreacting to a potential pathogen. One example is a phenomenon called “cytokine storm”. This occurrence takes place when the body is infected by specific pathogens. Some examples include the influenza virus that caused the 1918 Spanish Flu epidemic and more recently the SARS epidemic in What occurs during “cytokine storm” and how does it affect the host? Find an example of a more recent disease (hint: look at the flu virus) that induces cytokine storm.

 “Acute respiratory viral infection (especially from the H5N1 subtype influenza virus) results in a cytokine storm effecting the lungs, and subsequent damage to alveoli and lung tissue results in the lethality seen in more severe flu viral infections, especially those fatalities among young healthy adults” accessed July 21, 2011

Human society has evolved significantly over the past few decades. Populations of humans used to inhabit wide-open spaces and rarely had contact with large crowds of people. However, modern society requires people to live in very tight quarters and come into contact with possibly hundreds or thousands of people in a single day. Consider cities like New York City and Tokyo, Japan. Occupants in these cities are packed in tightly into subway systems and overcrowded conditions. When people in Japan are ill, they wear face masks to protect others, while in New York City this is not done. What are some other ways that people in these cities (and other large cities around the world) deal with potential exposure to pathogens?

 Microbiology encompasses many aspects of pathogens and human reactions to infections. This project will allow you to trace pathogens through the infection cycle and human response to the infection.  Project requirements: Refer to Table 1.1 and answer the following questions for EACH organism listed above. Word requirements are outlined for each question - this represents a minimum number of words required. For each question answer in full and complete sentences, and provide both examples and scientific evidence to support your answer. Be sure to include a well-developed introduction and conclusion in your paper.  You may use outside sources, but the reading material for Units 7 and 8 should provide the majority of information you will need to complete the assignment. Be sure to include all references in APA format.

OrganismStrategy to avoid host immune system Common disease Trypanosoma cruziChanges surface antigens to avoid immune system detection American trypanosomiasis Human immunodeficiency virus (HIV) Virus infects and hides in macrophages and CD4 T-cells. Acquired immunodeficiency syndrome (AIDS) Streptococcus pneumoniae Produce M proteins to inhibit phagocytes from attaching Pneumonia

1. Give a brief overview of the disease including signs and symptoms. Who is typically affected by this infection?(Children? Immunocompromised patients?)? What is the pathogens’ portal of entry and exit? (100 word minimum) 2. How would the innate immune system react to this pathogen? Which components would react? (75 word minimum) 3. Would this pathogen primarily stimulate cell-mediated immunity or antibody-mediated immunity (T cells or B cells?) Why? (75 word minimum) 4. All of the organisms listed above have the ability to evade the host immune system, at least temporarily. Review Table 1.1 for the pathogens’ strategies used to avoid the host’s immune system. Why is this particular strategy helpful to the pathogen? How would this impact the pathogens’ ability to cause infection? How would the immune system compensate? (200 word minimum)

 Tortora, G., Funke, B., & Case, C. (2007). Microbiology: An Introduction (8th ed.). San Francisco, CA: Pearson.