Biotechnology Development: Opportunities and Challenges Dr Prakash Ghimire, Ph D Associate Professor Microbiology Tribhuvan University Microbiologist, WHO-IPD/NPHL/EDCD-MoH

Ecological Zones and Development Regions of Nepal Far-Western Region Mid-Western Region Western Region Central Region Eastern Region Mountains (16 districts) Hills (39 districts) Terai (20 districts)

Biotechnology: Animal Pharmaceutical Plant Hybrids Diagnostic Therapeutic Vaccines Plant Agriculture - NARC Food

Biotechnology: Post Human genome situation Cell biology Immunology Proteomics & Genomics – two closely related area Cell biology Immunology Antibodies Polyclonal Monoclonal Molecular biology Genomics Proteomics

RONAST

RONAST ACTIVITIES: RESEARCH ACTIVITIES Biotechnology Programs - Conservation of Medicinal and Aromatic Plants [Click Here]- Molecular Diagnostic of Citrus greening Disease – Molecular Characterization of Medicinal plants  -Tea clones  -Bacillus thuringinsis – Flora of Nepal Project Research on Organic Farming -Antagonistic Study of Ectomycorrhiza -Study of the role of Endomycorrhiza Natural Products Research -Bioprospecting of Cordyceps sinensis-Biopesticides study program -Analytical services

Nepal Health Research Council Supports health Research Mostly in health system research Negligible investment in Biotechnology

Medical Biotechnology In Nepal Immunology: Vaccine Production Rabies Vaccine Production (Central Veterinary Research Laboratory), Tripureswor Antibody Production Everest Biotech Founded by biochemists at Oxford University, based in Oxfordshire, UK with a production laboratory established in Kathmandu. CDM TU- Non commercial/Academic Use Antibody Production, Purification…….. National Public Health Laboratory Health Services Flow cytometric assay ( FACS)

Medical Biotechnology Molecular Biology DNA Sequencing – Proposed at National Forensic Laboratory, Khumaltar PCR RONAST- Plant molecular biology CDMTU – Tropical diseases- Malaria, Leishmania etc. National Public Health Laboratory, Teku- SARS, Malaria, etc. VBDRTC, Hetauda- Malaria, Leishmania Mycobacterial research Laboratory, Lele- Tuberculosis, Leprosy Universal College/ RLAAB- Plant mol. Bio. Veterinary Research Laboratory, Tripureswor- Si-Gan Health Foundation, Maharajgunj Real Time PCR ( Proposed) National Public Health Laboratory, Teku Avian Influenza diagnostic service Cell Biology

Real-Time PCR

Challenges: Machines without consumables Academic & HMG Institutes Training of Human Resources Latest updates Research Culture Carrer prospects Research Funding & Collaboration NHRC ? Big researches- Survey type, No Laboratory capacity development in Nepal Availability of Consumables in local market Mostly not available, If available, costs 3-5 times of original cost Entrepreneurship Market

Hope for Future New University programs as per market requirements Medical technology Program Medical Microbiology Program Pharmaceutical Sciences Program Biotechnology Program Allied health Programs ( Radiology, Dentistry, Physiotherapy, Bio-medical Engineering, …….) Availability of funding in post WTO scenario Introduction of newer Pharmaceutical companies Availability of trained /cost effective HRs Worldwide market in post WTO situation Coming up of new diagnostic companies Positive Government Policy ?

A small truth to make our life 100% successful…… Formula for Success A small truth to make our life 100% successful…… A B C D E F G H I J 1 2 3 4 5 6 7 8 9 10 K L M N O P Q R S 11 12 13 14 15 16 17 18 19 T U V W X Y Z 20 21 22 23 24 25 26

47% 54% 70% 96% 98% LUCK 12+21+3+11 EFFORT 5+6+6+15+18+20 LOVE 12+15+22+5 54% EFFORT 5+6+6+15+18+20 70% KNOWLEDGE 11+14+15+23+12+5+4+7+5 96% HARDWORK 8+1+18+4+23+15+18+11 98% None of them makes 100%

Then what makes 100% Is it money? …No!!! Our Life 100% Successful Is it leadership? …No!!! Every problems has a solution, so perhaps Change our “ATTITUDE” It is ATTITUDE towards life and work that makes Our Life 100% Successful

ATTITUDE 1+20+20+9+20+21+4+5 100% Don’t you think so ?!?!?!?!

Thank You