DNA Nanostructures and Patterning Taylor Stevenson Nucleic Acid Engineering February 15 th, 2011

Agenda Structures –Origami –Branched Annealing –Nanotubes –Alternative Materials Patterning and Application –Combing –Origami as Scaffold –Micropatterning Nanotubes

Origami Structures Rothemund “Folding DNA to create nano-scale shapes and patterns” Nature Articles (2006)

Origami Structures Rothemund “Folding DNA to create nano-scale shapes and patterns” Nature Articles (2006)

Branched Annealing Structures Modified from Luo et.al. Nature Letters

Nano-Tube Structures Chen et. al. “Approaching The Limit: Can One DNA Oligonucleotide Assemble into Large Nanostructures?” (2006)

Other Forms of Nucleic Acid Braasch & Corey, Chemistry and Biology (2001)

Other Forms of Nucleic Acid Lin et. al. “Mirror Image DNA Nanostructures for Chiral Supramolecular Assemblies” Nano Letters (2009)

Combing Linear DNA for Nanowires Deng and Mao “DNA-Templated Fabrication of 1D Parallel and 2D Crossed Metallic Nanowire Arrays” Nano Letters (2003)

DNA Origami as a Scaffold RNA/DNA hybridization assays Ke et. al. “Self-Assembled Water-Soluble Nucleic Acid Probe Tiles for Label-Free RNA Hybridization Assays” Science (2008)

DNA Origami as a Scaffold Ke et. al. “Self-Assembled Water-Soluble Nucleic Acid Probe Tiles for Label-Free RNA Hybridization Assays” Science (2008)

DNA Origami as a Scaffold Rinker et. al. “Self-assembled DNA nanostructures for distance-dependent multivalent ligand–protein binding” Nature Letters (2008)

Micropatterning Nanotubes Lin et. al. “Functional DNA Nanotube Arrays: Bottom-Up Meets Top-Down” Ange Chem (2007)

2D Patterning Branched Annealing Structures

Rigidity of “monomers” affects 2/3D structure. Varying the length of red region produces different flexibilities.

Stiffness Flexibility 2D Patterning Branched Annealing Structures

Stiffness Flexibility 2D Patterning Branched Annealing Structures

Micropatterning Nanotubes