Laboratory Methods in Biotechnology Theory and practice of common biological laboratory methods: solution preparation, spectrophotometry, electrophoresis, DNA manipulations, and biological, chemical, and radiological safety

Idea Behind the Course Provide practical experiences in laboratory activities that will help students make the transition from content-based lower division courses to application orientated senior level courses. Provide practical experiences in laboratory activities that will help students make the transition from content-based lower division courses to application orientated senior level courses. Provide skills and dispositions needed to carry out laboratory research as part of a career in the life sciences. This includes undergraduate research, working as a technician, graduate research, and affiliated professions. Provide skills and dispositions needed to carry out laboratory research as part of a career in the life sciences. This includes undergraduate research, working as a technician, graduate research, and affiliated professions.

Role in Your Curriculum Electives: Molecular Techniques, Cell and Molec, Immunology, Path Micro, Microbial Phys, etc MCMB Specialty: Lab Methods, Micro, Molecular Genetics Core: Reasoning, Investigation, Cell Biology, Genetics,

Objectives To give students experience in dimensional analysis used in microbiology and cellular and molecular biology, and biotechnology mmol mM gg

Objectives To familiarize the students with the theory and practice of preparing solutions from various starting materials. To familiarize the students with the theory and practice of preparing solutions from various starting materials.

Objectives To introduce the students to means of preparing sterile media and solutions.

Objectives To allow students to practice quantifying bacteria and viruses, and to measure the growth rates of bacterial populations. To allow students to practice quantifying bacteria and viruses, and to measure the growth rates of bacterial populations.

Objectives To allow students to quantify proteins, nucleic acids and other chemicals in solution. To allow students to quantify proteins, nucleic acids and other chemicals in solution.

Objectives To introduce students to commonly used devices including, but not limited to: microliter pipetters, centrifuges, spectrophotometers, pH meters, etc. To introduce students to commonly used devices including, but not limited to: microliter pipetters, centrifuges, spectrophotometers, pH meters, etc.

Objectives To introduce the students to the use of enzymes for end-point determination and to the measurement of enzyme activity.

Objectives To give students experience in the techniques for the manipulation and electrophoretic separation of proteins and DNA.

Objectives To provide information regarding biological, chemical, radiation, and other aspects of lab safety.

Textbooks Make sure you bring this each time. Make sure you bring this each time. Seidman and Moore – our main text Seidman and Moore – our main text Leboffe and Pierce – illustration of techniques Leboffe and Pierce – illustration of techniques Also need: Also need: Calculator! Calculator! Lab Notebook Lab Notebook

Additional Resources Brock – background information on theory Day – advice on writing (humor) Robyt - Source of many handouts/additional readings CBE Manual – Style guide for preparation of manuscripts Don’t buy these

Webpage Lab Activities Lab Activities Lecture Topics Lecture Topics Homework Assignments Homework Assignments In Class Assignments In Class Assignments Manuscripts Manuscripts Notebook Guidelines Notebook Guidelines Syllabus Syllabus Grade Book Grade Book