Yin-Yang Column for Global Protein Identification Column Technology Inc.

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Yin-Yang Column for Global Protein Identification Column Technology Inc.

Yin-Yang MDLC-MS/MS The Yin-Yang column combines anion and cation exchange chromatography For the protein identification through multi-LC/MS/MS:

Advantages of Yin-yang MDLC-MS/MS ► Three-dimensional LC-MS/MS ► SCX and ANX are complimentary methods for peptide fractionation ► Most complete coverage of peptides and proteins ► Enrichment and high-sensitivity for phosphopeptides ► Identify phospho and non-phospho peptides in the same run.

Theoretical pI distribution of identified non-phosphrylated peptides Complementary peptide identification of SCX and SAX

SAXSCX Unique peptides 6184 Proteins SAX SCX only 1mg protein digests of mouse liver, 5% FPR Another More than 1000 phosphopeptides identified