Bio 498/698: January 27, 2012

Lecture Outline Computer Start up P-51 Camlift Controls Lens setup Live View Moving the Camlift Taking a picture Autorun

Computer Start Up 1: Press red button on power-strip 2: Press power button to start computer 3: When prompted (it will take a while), press CTRL-alt- delete 4: To log in, use the username bio2\user. No password needed, leave it blank and press enter. To open the 4 needed programs, you can use the programmed buttons on the far left of the keyboard. M1 = Opens the Camlift M2 = Opens Lightroom M3 = Opens Zerene Stacker M4 = Opens Photoshop

The P-51 Camlift

Camlift Controller When you open the lift (Key “M1”), click “OK” when prompted.

Camlift Controls

Setting Up the Lens ALWAYS SET UP LENS BEFORE: 1.Camera is set up on lift or, 2.A lens is changed Scroll over Settings and click on “Edit Step Settings”

Setting Up the Lens, continued With Step Settings window open, click on “Set Up Lens”

Setting Up the Lens, continued In Set Up Lens window, click on lens and aperture (F#) being used. Click “Save” In Step Settings window, click “Apply” then “Ok”

Live View To open Live View click on the ‘Live View’ button on the Camlift control panel You’ll hear a click from the camera and a window will open, displaying everything the camera “sees”. Live View

Grid Select Magnify White Balance Point of Interest Outline

Live View Drag the point of interest outline to the desired position. In this example it is in the center, surrounding the words Live View Test. Click on the Magnify button Magnify

Live View Magnified view (500%) To zoom out, hit the de-magnify button. De-magnify

Moving the Camlift up and down The Up and Down buttons: The amount you move the Camlift up or down is governed by the step size you have selected with the ‘Step Size Slider’. Gross Movement Slider Step Size Slider Emergency stop button Up and Down buttons

Moving the Camlift up and down Gross Movement Slider Notice: As the Gross Movement Slider is in a lower position, the image of the specimen is clearer

Moving the Camlift up and down The Stop Button: If the Camlift is moving and is in danger of hitting something, this will stop it The ‘Infinite’ Step size: allows the lift to move up or down continuously when held down Other step sizes: move up/down once per click

Taking a picture Always close out of Live View before taking a picture! Step 1: Close out of Live View Step 2: Make sure Lightroom is open Step 3: Click on the ‘Take Picture’ button Step 4: The picture should now be visible in Lightroom Take Picture Take Picture button = Click this when the camera is connected and turned on and it will fire the camera and flash

Autorun: Taking Slices At the highest point of the specimen that is in focus, click the ‘Start Here’ button Start Here Highest in- focus point

Autorun: Taking Slices Stop Here Lowest in- focus points At the lowest in-focus point on the specimen, click the ‘Stop Here’ button

Autorun: Taking Slices When ‘Start Here’ and ‘Stop Here’ positions have been designated, click the ‘Autorun’ button Make sure the Current Lens Magnification is the same as the yellow magnification on the lens is the same Look at the Number of Slices (Try to keep the # under 25) Hit the ‘Start button’ *Opening Autorun will close out of Live View # Slices Current lens Magnification

Autorun Congratulations! You have now taken your first set of photographs. Be sure to check the Lightroom and Zerene Stacker guides!