Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies, LLC
Lab-on-chip approach Simplicity of operation Reduction of moving parts – no (mechanical) pumps Robustness Inexpensive and facile maintenance
Chip Layout 20mm Column Inlet lines Detector electrodes
From Old Setup…
…To New NeSSI Mountable Design
Split flow approach to sampling Liters per minute microliters per minute nanoliters per minute
Fluid Flow Strategy
Functional Integration of -fluidic LC Chip for On-line Sample Pretreatment micromixer diluent in column mobile phase in sample in
Rapid Prototyping Using Quick Photolithographic Method Inkjet printed photomaskFinished product
Front Mounted Microfluidic Chip
Back-Mounted Valves
Quantitation of flow splitting