CHROMATOGRAPHY AP BIO LAB #4A Image by Riedell

PLANT PIGMENTS.

CHROMATOGRAPHY USE PENCIL not pen, to mark starting line !!!!! Image by Riedell

ADD PLANT PIGMENTS Image from Biology Place:

PLACE IN JAR Image by Riedell IMPORTANT: Make sure tip of paper is touching solvent Make sure pigment line is ABOVE solvent Put lid on jar !!!!!!!!

Important Don’t let solvent front run off end of paper !

Marker Chromatography Image by Riedell

R f = Distance pigment migrated Distance solvent front migrated Image by Riedell

PLANT PIGMENTS Carotene is orange yellow Xanthophyll is lemon yellow Chlorophyll a is blue green Chlorophyll b is yellow green. Image by Riedell

Factors that affect movement of molecules during chromatography 1.___________of molecule in solvent used (better it dissolves… farther it will move) Solubility SOLVENT used: Chlorophyll: 9:1 Petroleum ether : acetone Markers: water

Factors that affect movement of molecules during chromatography 2. _________of molecule (bigger molecules move slower) Size

Factors that affect movement of molecules during chromatography 3. _______________________ used (EX: Attraction of molecules to paper) Attraction to media Lots of different kinds of chromatography

Factors that affect movement of molecules during chromatography 4. __________allowed to run Time Longer time = More separation

CHLOROPHYLL FLUORESCENCE Image from Biology AP Edition by Campbell & Reece published by Pearson Benjamin Cummings