HANDS-ON APPROACH TO CHROMATOGRAPHY Nataša Gros 1 Margareta Vrtačnik 2, M. Filomena Camões 3 1 Faculty of Chemistry and Chemical Technology, 2 Faculty of Natural Sciences and Engineering, University of Ljubljana, Slovenia 3 University of Lisbon, Portugal Project No: SI/03/B/F/PP © FKKT, Ljubljana,

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1. Introduction to chromatographyIntroduction to chromatography 2. Gas chromatographyGas chromatography 3. Liquid chromatographyLiquid chromatography 4. Chromatography –fields of applicationChromatography –fields of application

Examples of slides

A simplified gas chromatograph Cylinder with carrier gas Flow regulator Injector Column with stationary phase Detector display  Record of measurement values

Chromatogram Graphic presentation of measurement values vs time Chromatographic peak of the component eluted first Chromatographic peak of the component eluted second RESPONSERESPONSE

A simplified liquid chromatograph

Chromatographic system with vacuum water-jet pump Container with eluent Pump Sample injection port Column with stationary phase Detector display  Record of measurement values

“ Separating the dyes from mint aroma Video: click on the icon

“ Separation of the dyes from mint aroma Green component blue component

Acknowledgements This Project has been carried out with the support of the European Community. The content of this project does not necessarily reflect the position of the European Community, or National Agency. For this reason neither the National Agency nor the European Community take any responsibility regarding the content of the report.