1 POPcorn: Project Portal for corn A set of project and sequence-indexed data searching resources ( Jack M. Gardiner Poster P897

POPcorn: Project Portal for corn A set of project and sequence-indexed data searching resources

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POPcorn: Project Portal for corn 4 Project Objectives: Difficult to find all online resources relevant to ones research Project Search Project Objectives: Difficult to find all online resources relevant to ones research Project Search

POPcorn: Project Portal for corn 5 Project Objectives: Difficult to find all online resources relevant to ones research Project Search Repeated BLAST searches at multiple sites POPcorn BLAST Project Objectives: Difficult to find all online resources relevant to ones research Project Search Repeated BLAST searches at multiple sites POPcorn BLAST

POPcorn: Project Portal for corn 6 Project Objectives: Difficult to find all online resources relevant to ones research Project Search Repeated BLAST searches at multiple sites POPcorn BLAST Sequence-indexed data can be challenging to search Sequence-Indexed Data Search Project Objectives: Difficult to find all online resources relevant to ones research Project Search Repeated BLAST searches at multiple sites POPcorn BLAST Sequence-indexed data can be challenging to search Sequence-Indexed Data Search

POPcorn: Project Portal for corn 7 Project Objectives: Difficult to find all online resources relevant to ones research Project Search Repeated BLAST searches at multiple sites POPcorn BLAST Sequence-indexed data can be challenging to search Sequence-Indexed Data Search Data and web resources can disappear after project completion Processes and scripts to migrate data to MaizeGDB Project Objectives: Difficult to find all online resources relevant to ones research Project Search Repeated BLAST searches at multiple sites POPcorn BLAST Sequence-indexed data can be challenging to search Sequence-Indexed Data Search Data and web resources can disappear after project completion Processes and scripts to migrate data to MaizeGDB

POPcorn: Project Portal for corn 8 Project Search Database of maize research projects and online resources Hand-curated and updated monthly; 97 projects, 128 resources Project Search Database of maize research projects and online resources Hand-curated and updated monthly; 97 projects, 128 resources

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POPcorn: Project Portal for corn 12 Project Search Database of maize research projects and online resources Hand-curated and updated monthly; 97 projects, 128 resources We need your help! submit projects not in our database send us corrections Project Search Database of maize research projects and online resources Hand-curated and updated monthly; 97 projects, 128 resources We need your help! submit projects not in our database send us corrections

POPcorn: Project Portal for corn 13 Project Search Database of maize research projects and online resources Hand-curated and updated monthly; 97 projects, 128 resources Information presented for each project and resource We need your help! submit projects not in our database send us corrections Types of projects and resources: just about everything that is relevant to maize … except cultural, archeological and educational; those go into PGROP at PlantGDB ( Project Search Database of maize research projects and online resources Hand-curated and updated monthly; 97 projects, 128 resources Information presented for each project and resource We need your help! submit projects not in our database send us corrections Types of projects and resources: just about everything that is relevant to maize … except cultural, archeological and educational; those go into PGROP at PlantGDB (

POPcorn: Project Portal for corn 14 Two options for searching with a sequence Simple sequence to sequence search (BLAST) Sequenced index search (access biological resources associated with a sequence) Two options for searching with a sequence Simple sequence to sequence search (BLAST) Sequenced index search (access biological resources associated with a sequence)

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POPcorn: Project Portal for corn 16 BLAST against target sequence at multiple websites using Web Services Results can be returned via BLAST against target sequence at multiple websites using Web Services Results can be returned via

POPcorn: Project Portal for corn 17 BLAST against target sequence at multiple websites using Web Services Results can be returned via Contact us to add your target sequence or to set up a similar service BLAST against target sequence at multiple websites using Web Services Results can be returned via Contact us to add your target sequence or to set up a similar service

POPcorn: Project Portal for corn 18 Sequence-indexed data search Carries out complex searches One or more searches at once Sequence-indexed data search Carries out complex searches One or more searches at once

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20 POPcorn: Project Portal for corn An example: steps taken to find a UniformMu stock containing a given mutation. 1.BLAST query sequence against NCBI GSS 2.Parse definition line to get UniformMu locus name 3.Get LOCUS record at MaizeGDB 4.Get VARIATION record(s) for that LOCUS at MaizeGDB 5.Get UniformMu STOCK records for each VARIATION at MaizeGDB 6.Construct links to MGC Stock Center for each stock found.

21 POPcorn: Project Portal for corn An example: steps taken to find a UniformMu stock containing a given mutation. 1.BLAST query sequence against NCBI GSS 2.Parse definition line to get UniformMu locus name 3.Get LOCUS record at MaizeGDB 4.Get VARIATION records for that LOCUS at MaizeGDB 5.Get UniformMu STOCK records for each VARIATION at MaizeGDB 6.Construct links to MGC Stock Center for each stock found.

22 POPcorn: Project Portal for corn Select a target database Input your sequence (split files larger than 20MB) Provide some information about your data Submit! uBLAST

23 POPcorn: Project Portal for corn Monitor progress of your job You will receive an when your job is complete uBLAST

24 POPcorn: Project Portal for corn Check your alignment on the MaizeGDB.org genome browser If the results look correct, submit to MaizeGDB.org uBLAST

25 POPcorn: Project Portal for corn CViT – Chromosome Viewing Tool Displays features on chromosomes (linkage groups, pseudomolecules, chromosome arms, et cetera)

26 POPcorn: Project Portal for corn CViT – Chromosome Viewing Tool

27 POPcorn Principal Investigator: Carolyn Lawrence (USDA-ARS) Co-Principal Investigator: Taner Sen (USDA-ARS) Solution/Application Architect: Ethy Cannon (Iowa State) Programmer Analyst: Scott Birkett (Iowa State) Programmer Analyst: Bremen Braun (USDA-ARS) Curator: Jack Gardiner (Iowa State) POPcorn Principal Investigator: Carolyn Lawrence (USDA-ARS) Co-Principal Investigator: Taner Sen (USDA-ARS) Solution/Application Architect: Ethy Cannon (Iowa State) Programmer Analyst: Scott Birkett (Iowa State) Programmer Analyst: Bremen Braun (USDA-ARS) Curator: Jack Gardiner (Iowa State) MaizeGDB/POPcorn team Poster P897