1 Intro to Mendelelian Genetics 2 Gregor Mendel (1822-1884) Responsible for the Laws governing Inheritance of Traits.

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Presentation transcript:

1 Intro to Mendelelian Genetics

2 Gregor Mendel ( ) Responsible for the Laws governing Inheritance of Traits

3 Gregor Johann Mendel

4  Austrian monk  Between 1856 and 1863, Mendel cultivated and tested some 28,000 pea plants  He found that the plants' offspring retained traits of the parents.  Called the “Father of Genetics"

5  Mendel stated that physical traits are inherited as “particles”  Mendel did not know that the “particles” were actually Chromosomes & DNA Particulate Inheritance

6 Genetic Terminology  Trait - any characteristic that can be passed from parent to offspring  Heredity - passing of traits from parent to offspring  Genetics - study of heredity

7 Types of Genetic Crosses  Monohybrid cross - cross involving a single trait e.g. flower color  Dihybrid cross - cross involving two traits e.g. flower color & plant height

8 Punnett Square  Used to help solve genetics problems

9

10 Designer “Genes”  Alleles - two forms of a gene (dominant & recessive)  Dominant - stronger of two genes expressed in the hybrid; represented by a capital letter (R)  Recessive - gene that shows up less often in a cross; represented by a lowercase letter (r)

11  Genotype - gene combination for a trait (e.g. RR, Rr, rr)  Phenotype - the physical feature resulting from a genotype (e.g. red, white)

12 Genotype & Phenotype in Flowers Genotype of alleles: R = red flower r = yellow flower All genes occur in pairs, so 2 alleles affect a characteristic Possible combinations are: GenotypesRR Rrrr PhenotypesRED RED YELLOW

13 Genotypes  Homozygous genotype - gene combination involving 2 dominant or 2 recessive genes (e.g. RR or rr); also called pure  Homozygous genotype - gene combination involving 2 dominant or 2 recessive genes (e.g. RR or rr); also called pure  Heterozygous genotype - gene combination of one dominant & one recessive allele (e.g. Rr); also called hybrid

14 Genes and Environment Determine Characteristics

15 Mendel’s Pea Plant Experiments

16 Why peas,Pisum sativum?  Can be grown in a small area  Produce lots of offspring  Produce pure plants when allowed to self-pollinate several generations  Can be artificially cross-pollinated

17 Reproduction in Flowering Plants Pollen contains sperm Produced by the stamen Ovary contains eggs Found inside the flower Pollen carries sperm to the eggs for fertilization Self-fertilization can occur in the same flower Cross-fertilization can occur between flowers

18 How Mendel Began Mendel produced pure strains by allowing the plants to self- pollinate for several generations

19 Eight Pea Plant Traits Seed shape --- Round (R) or Wrinkled (r) Seed Color ---- Yellow (Y) or Green (y) Pod Shape --- Smooth (S) or wrinkled (s) Pod Color --- Green (G) or Yellow (g) Seed Coat Color ---Gray (G) or White (g) Flower position---Axial (A) or Terminal (a) Plant Height --- Tall (T) or Short (t) Flower color --- Purple (P) or white (p)

20

21

22 Mendel’s Experimental Results

23 Generation “Gap” Parental P 1 Generation = the parental generation in a breeding experiment. F 1 generation = the first-generation offspring in a breeding experiment. (1st filial generation) From breeding individuals from the P 1 generation F 2 generation = the second-generation offspring in a breeding experiment. (2nd filial generation) From breeding individuals from the F 1 generation From breeding individuals from the F 1 generation

24 Following the Generations Cross 2 Pure Plants TT x tt Results in all Hybrids Tt Cross 2 Hybrids get 3 Tall & 1 Short TT, Tt, tt

25 Monohybrid Crosses

26 Trait: Seed Shape Alleles: R – Roundr – Wrinkled Cross: Round seeds x Wrinkled seeds RR x rr P 1 Monohybrid Cross R R rr Rr Genotype:Rr Genotype: Rr PhenotypeRound Phenotype: Round Genotypic Ratio:All alike Genotypic Ratio: All alike Phenotypic Ratio: All alike

27 P 1 Monohybrid Cross Review  Homozygous dominant x Homozygous recessive  Offspring all Heterozygous (hybrids)  Offspring called F 1 generation  Genotypic & Phenotypic ratio is ALL ALIKE

28 Trait: Seed Shape Alleles: R – Roundr – Wrinkled Cross: Round seeds x Round seeds Rr x Rr F 1 Monohybrid Cross R r rR RR rrRr Genotype:RR, Rr, rr Genotype: RR, Rr, rr PhenotypeRound & wrinkled Phenotype: Round & wrinkled G.Ratio:1:2:1 G.Ratio: 1:2:1 P.Ratio: 3:1

29 F 1 Monohybrid Cross Review  Heterozygous x heterozygous  Offspring: 25% Homozygous dominant RR 50% Heterozygous Rr 25% Homozygous Recessive rr  Offspring called F 2 generation  Genotypic ratio is 1:2:1  Phenotypic Ratio is 3:1

30 What Do the Peas Look Like?

31 …And Now the Test Cross Mendel then crossed a pure & a hybrid from his F 2 generation This is known as an F 2 or test cross There are two possible testcrosses: Homozygous dominant x Hybrid Homozygous recessive x Hybrid

32 Trait: Seed Shape Alleles: R – Roundr – Wrinkled Cross: Round seeds x Round seeds RR x Rr F 2 Monohybrid Cross (1 st ) R R rR RR RrRR Rr Genotype:RR, Rr Genotype: RR, Rr PhenotypeRound Phenotype: Round Genotypic Ratio:2:2 Genotypic Ratio: 2:2 Phenotypic Ratio: All alike

33 Trait: Seed Shape Alleles: R – Roundr – Wrinkled Cross: Wrinkled seeds x Round seeds rr x Rr F 2 Monohybrid Cross (2nd) r r rR Rr rrRr rr Genotype:Rr, rr Genotype: Rr, rr PhenotypeRound & Wrinkled Phenotype: Round & Wrinkled G. Ratio: G. Ratio: 2:2 P.Ratio: 2:2

34 F 2 Monohybrid Cross Review  Homozygous x heterozygous(hybrid)  Offspring: 50% Homozygous RR or rr 50% Heterozygous Rr  Phenotypic Ratio is 1:1  Called Test Cross because the offspring have SAME genotype as parents

35 Mendel’s Laws

36 Results of Monohybrid Crosses  Inheritable factors or genes are responsible for all heritable characteristics  Phenotype is based on Genotype  Each trait is based on two genes, one from the mother and the other from the father  True-breeding individuals are homozygous (both alleles are the same)

37 Law of Dominance In a cross of parents that are pure for contrasting traits, only one form of the trait will appear in the next generation. All the offspring will be heterozygous and express only the dominant trait. RR x rr yields all Rr (round seeds)

38 Law of Dominance

39 Law of Segregation During the formation of gametes (eggs or sperm), the two alleles responsible for a trait separate from each other. Alleles for a trait are then "recombined" at fertilization, producing the genotype for the traits of the offspring Alleles for a trait are then "recombined" at fertilization, producing the genotype for the traits of the offspring.

40 Applying the Law of Segregation

41 Law of Independent Assortment  Alleles for different traits are distributed to sex cells (& offspring) independently of one another.  This law can be illustrated using dihybrid crosses.

42 Dihybrid Cross A breeding experiment that tracks the inheritance of two traits. Mendel’s “Law of Independent Assortment” a. Each pair of alleles segregates independently during gamete formation