The balancing act. Redox poise and signalling

Acknowledgements Lund University Plant Cell Biology-Plant Biochemistry Thomas Pfannschmidt (Now University of Jena, Germany) Dalibor Stys (Now S. Bohemia University, Czech Republic) Anders Nilsson (Now Bruker Spectroscopy, Lund) Sylvan Dubé (Now Laval University, Canada) Paul Davison (Now Sheffield University, U.K.) Jens Forsberg Jörgen Ström Magnus Rosenquist (Sommarin lab) Laure Fraysse (Kjellbom lab) H. Louise Race (Now Aylesbury, U.K.) Collaborators Torbjörn Drakenberg, Sture Forsén (Lund Physical Chemistry 2) Gerhard Link (University of Bochum, Germany) James A. Sullivan, John C. Gray (University of Cambridge, U.K.) Cartoons Sarah Allen

The bucket

The searchlight Allen, J. F. (2001) EMBO Reports 2,

Light 2 Light 1

The balancing act 1. Transients, enhancement, and redox poise of cyclic electron flow. Rates and activities of components.

Myers, J. (1971) Annu. Rev.Plant Physiol. 22,

Hill, R. (1965) Essays Biochem. 1,

Park, R. B. and Sane, P.V. (1971) Annu. Rev. Plant Physiol.22,

Albertsson, P.-Å. (2001) Trends Plant Sci. 6,

Pfannschmidt, T. et al. (1999) IUBMB Life 48,

The balancing act 2. Protein phosphorylation and state transitions. Post-translational modification of the structure and function of proteins you already have.

Allen, J. F. (1992) BBA 1098, Adapted from Allen, J.F. et al. (1981) Nature 291, 25-29

Updates on state transitions Wollman, F.-A. (2001) EMBO J. 20, –“The kinase” and the site of redox control Kruse, O. (2001) Naturwissenschaften 88, –Structure and the PS II-LHC II supercomplex Haldrup, A. et al. (2001) Trends Plant Sci., 6, –Binding change, PS I and Psa-H

Dubé, S., Davison, P.A. and Allen, J. F. (unpublished)

State 1State 2

State 1State 2 Allen, J. F. and Forsberg, J. (2001) Trends Plant Sci.6,

Nilsson, A. et al. (1997) JBC 272,

The balancing act 3. Redox control of gene expression. Changing transcription and the stoichiometry of complexes.

Allen, J. F. (1992) BBA 1098,

Pfannschmidt, T. et al. (1999) IUBMB Life 48,

Pfannschmidt, T. et al. (1999) Nature 397,

The balancing act 4. Redox signalling and evolution. Why chloroplasts (and mitochondria…) have genomes

Allen, J. F. (1993) J. Theor. Biol. 165,

Localization of Arabidopsis BAB09274 His Kinase-GFP fusion protein in tobacco leaf epidermal cells Unpublished data of Sullivan, J. A., Gray, J.C. (Cambridge) Rosenquist, M., Forsberg, J., Allen, J. F. (Lund)

Protein IDOrganismType of two- component signalling element Predicted subcellular locationProbability of location ycf26Porphyra purpureaSensor histidine kinaseChloroplast1* BAB09274Arabidopsis thalianaHybrid histidine kinaseChloroplast thylakoid membrane AAD03576Arabidopsis thalianaHybrid histidine kinaseChloroplast thylakoid membrane ycf27Porphyra purpureaResponse regulatorChloroplast1* ycf29Porphyra purpureaResponse regulatorChloroplast1* AAD15535Arabidopsis thalianaResponse regulatorMitochondrial matrix space0.628 AAD55287Arabidopsis thalianaResponse regulatorChloroplast stroma0.864 BAB20581Zea maysResponse regulatorChloroplast stroma0.938 BAA85112Zea maysResponse regulatorChloroplast thylakoid membrane BAA85113Zea maysResponse regulatorChloroplast stroma0.600 Two-component sensors and response regulators predicted to be in chloroplasts and mitochondria (by P-sort) Forsberg, J. et al. (2001) Biochem. Soc. Trans. 29,

Race, H. L., Herrmann, R. G. and Martin, W. (1999) Trends Genet. 15,

The balancing act. Redox poise and signalling Transients, enhancement, and redox poise of cyclic electron flow. Protein phosphorylation and state transitions. Redox control of gene expression. Redox signalling and evolution.

Light 1 Light 2

The balancing act. Redox poise and signalling