New Functions For Plant PR-5 (Thaumatin-like) Proteins

Ubiquitous Plant PR-5 Protein Facts Osmotin mRNA per 10 ug total RNA La Rosa et al., (1992) Plant Physiol. 100:409

(De Vos et al., 1995) C N N C * * * * * * * * (PDB) (Koiwa et al., 1997) PR-5 Proteins Have Conserved Structure **** *** *** * ** ** * Thaumatin, Osmotin TLPs, OLPs

Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. ‘Samsun’ and ‘Samsun NN’ II. Changes in protein constitution after infection with tobacco mosaic virus L. C. Van Loon and A. Van Kammen Laboratory of Virology, State Agricultural University, Wageningen, The Netherlands Accepted 14 August Available online 16 April Abstract Electrophoresis in 5, 7.5, and 10% acrylamide gels revealed that characteristic changes appear in the protein constitution of N. tabacum var. ‘Samsun,’ ‘Samsun NN,’ and N. glutinosa after infection with tobacco mosaic virus (TMV). Apart from significant quantitative changes, one new band was present in Samsun plants 4 weeks after infection. This band was characterized as TMV coat protein by coelectrophoresis and serology. A change in the electrophoretic mobility of the major band was recorded. In Samsun NN plants four new bands (I–IV) were present 1 week after infection. These bands are not related to TMV coat protein and are not new isozymes of peroxidase, polyphenoloxidase, acid phosphatase, glucose-6-phosphate dehydrogenase, or 6-phosphogluconate dehydrogenase. The bands first appear at the onset of necrosis and increase in intensity with time. Five days after infection band I ceases to increase, whereas bands II, III, and IV increase up to day 14. From day 7 onward, the four bands are also present in the noninoculated, young developing leaves, where they further increase with time. The amount of the four new protein components was related to lesion density and was reduced by treatment of the leaves with actinomycin D 2 days after infection. In N. glutinosa one new band was induced and two bands increased markedly after infection, while one had disappeared from the protein pattern. These changes relate to other protein components than those found in infected Samsun NN, but are also apparent in the noninoculated parts of the plants from day 7 after infection. The possible relation of the new protein components to the phenomenon of systemic acquired resistance is discussed. Virology Volume 40, Issue 2Virology Volume 40, Issue 2, February 1970, Pages

PathogenPerception Signal Transduction RIP PR-2s PR-1s PR-5s LTPs PR-14 Proteinase inhibitors PR-6 Thionins PR-13 Defensins PR-12 Chitinases PR-3,-4, -8, -11 Defense genes encoding antimicrobials PR-5 Proteins Are Plant Defense Effectors In Fond Memory Pathogen PR-7 RNAse PR-10 Other

Transgenic Overexpression Of PR-5 Genes Increases Stress Tolerance Plant speciesPR-proteinDonorStressorTolerance (change a ) Agrostis palustrisPR-5Oryza sativaSclerotinia homeocarpa + Brassica napusPR-5Hordeum vulgarePlasmodiophora brassicae + Citrus sinensisPR-5Solanum lycopersicumPhytophthora citrophthora + Daucus carotaPR-5Or. sativa Alternaria,Aternaria, Alternaria, Botrytis, Rhizoctonia, Sclerotinia,, Sclerotium + Dianthus caryophyllusosmotinNicotiana tabacumFusarium + Fragaria x ananassathaumatin osmotin Thaumatococcus daniellii Ni. tabacum Botrytis salt ++++ Gossypium hirsutumosmotinNi. tabacumdrought + (greenhouse) Hyacinthus orientalisthaumatinTh. danielliiiFusarium culmorum + Nicotiana tabacumosmotin thaumatin PR-5 Ni. tabacum Th. daniellii Or. sativa drought, salt Pythium, Rhizoctonia, Alternaria Olea europaeaosmotinNi. tabacumcold + Oryza sativaPR-5 Chitinase + TLP + Serine-threonine kinase Or. sativa Rhizoctonia Sarocladium, Xanthomonas ++++ Solanum lycopersicumosmotin + chitinase thaumatin Ni. tabacum, Phaseolus vulgaris Th. daniellii Fusarium Phytophthora + (greenhouse) Solanum tuberosumosmotinNi. tabacumPhy. infestans + Triticum aestivumPR-5 osmotin Or. sativa Ho. vulgare Ni. tabacum Fusarium salt - ( field) + (geenhouse) +

Arabidopsis thaliana TLPs: characteristics and tissue-specific expression. Adv Bot Res (2009) 17:

Induction Of Arabidopsis TLPs By By Stress, Senescence And Nutrients Adv Bot Res (2009) 17:

The PR-5 Protein Gene Family In A Plant Species Is Fairly Large SpeciesArabidopsisRicePoplarGrape Loci (#) Adv Bot Res (2009) 17:

Occurence of PR-5/Thaumatin-like Proteins Sequence and structural similarity with plant PR-5 proteins As families YesNo Flowering plants (All) Mosses (All) Algae (All) Amoeba (Some) Fungi (Most) Insects (Some) Bacteria (Some) Cyanobacteria Viruses Mammals (All) Bony fishes (All) Birds (All) Species Adv Bot Res (2009) 17: