Lienhard Pagel Flow injection analysis realized using PCBs S. Gassmann, L. Pagel Faculty of computer since and electronics Institute of Electronic Appliances and Circuits, University of Rostock, Rostock, Germany

Lienhard Pagel Micro fluidics in PCB technology

Lienhard Pagel University of Rostock PCB-Fluidics

Lienhard Pagel Fluidic PCB-Technology normal PCB production drilling milling

Lienhard Pagel Fluidic PCB-Technology normal PCB production etching

Lienhard Pagel Fluidic PCB-Technology second PCB stacked copper lines build channels gluing

Lienhard Pagel Fluidic PCB-Technology tube connection channel view

Lienhard Pagel Fluidic PCB-Technology cross section of channel

Lienhard Pagel Cross section of channel wall epoxy copper (2) copper (1) channel

Lienhard Pagel µC controlled programmable micro pump  Electrical connector - Power 4 –7 V DC - I 2 C - Bus - programming  500µl/min Flow  150 mBar max Backpressure  over USB-I2C IF fully programmable

Lienhard Pagel Flow-Injection-Analysis quantitative measurement of components automated procedure short response times / high sample throughput usage in quality control / automated assays developed by J. Růžička and E.H. Hansen in the year 1975

Lienhard Pagel Flow-Injection-Analysis Sample Reaction coil Injection valveDetector Reagent Pump

Lienhard Pagel Reaction Detection of Fe 3+ Thiocyanate as reagent forms red colored Iron-Thiocyanate detection by absorption at 480 nm

Lienhard Pagel Why a FIA? All basic fluidic handling necessary - transportation of fluids - dosing of fluids - mixing - detecting - adoption to detection needed - electronics needed

Lienhard Pagel FIA in PCB-Technologie Principle reagent pump sample pump reaction coil injectiondetector 2 separate controllable pumps no injection valve optical detection photometrical 480 nm

Lienhard Pagel MIXING Flow pattern in 180° curves

Lienhard Pagel Simulation results of the used reaction channel Parameters: 2 pumps with constant flow of 0.2ml/min Fluidsimulation: FLOWORKS cells

Lienhard Pagel … and the reality

Lienhard Pagel Simulation results of the used reaction channel parameters: thermopneumatically actuated pumps with pulsing flow of 0.2ml/min, cells

Lienhard Pagel Simulation results of the reaction channel vector plot of the fluid velocity at the cross section of the channel after the second curve parameters: thermopneumatically actuated pumps with pulsing flow of 0.2ml/min, cells

Lienhard Pagel OPTICAL SENSOR OPTICAL SENSOR in FIA

Lienhard Pagel Optical Cell with air bubble (vector of the force)

Lienhard Pagel Realization 5 cm stack of four PCBs one movable layer of polyimid electronics on the back of the fourth PCB

Lienhard Pagel FIA Realization

Lienhard Pagel FIA in PCB with integrated electronics

Lienhard Pagel Signals in the photo detector in dependence on the concentration of Fe(III)ions

Lienhard Pagel PCB FIA facts Detection Fe 3+ Ions 0,16 mMol – 10 mMol reproducibility peak height 5%, peak area 10% Monolithic system consisting fluidics and electronics in one stack of PCBs.

Lienhard Pagel Thank you for your attention!