DNA Marker Validation Update Dick Quaas Cornell University Beef improvement Federation Annual Meeting, Billings MT, 7-9 July 2005.

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Presentation transcript:

DNA Marker Validation Update Dick Quaas Cornell University Beef improvement Federation Annual Meeting, Billings MT, 7-9 July 2005

Overview Merial SNPs in validation pipeline Currently genotyping Bovigen Several SNPs analyzed 1 more coming Today’s topic

Bovigen Genotypes/Tests Tenderness = Warner-Bratzler Shear Force Calpastatin (CAST)Australia Calpain 316 (CAPN316)MARC GeneStar Tenderness 2 = CAST + CALP316 (0-4) Quality = Marbling Score TG5: original GeneStar Marbling M2: new anonymous marker M3: new anonymous marker Feedlot Quality = TG5+M2+M3 (scored 0-6) GeneStar Quality Test = TG5+M2 (scored 0-4)

Data: Cattle Continental Charolais–sired CMP cattle “with a few exceptions,... black Angus females ” Robert Williams British Hereford–sired CMP cattle “majority of the... dams were Hereford or Hereford x Red Angus crosses” Craig Huffines Indicus-influenced purebred Santa Gertrudis cattle from King Ranch – testing in progress

Tenderness Analyses Models Y = CG + Marker Effect + Sire + e Marker Effect 1.b x (CAST + CAPN316) b = effect of average tenderness star 2.b1 x CAST + b2 x CAPN316 b1 =effect of CAST star b2 = effect of CAPN316 star SAS Proc-Mix

Value of a Tenderness Star

Tenderness Taurus Data

Conclusions: GeneStar Tenderness Combined Taurus data CAST, CAPN316 & GeneStar Tenderness 2 effects 1.all statistically significant 2.all biologically important 3.CAPN316 star bigger than CAST star

Marbling Analyses Traits Marbling Score “% Choice” = % ≥ Small00 Model Y = CG + Marker Effect + Sire + e Marker Effect 1.b x GSQT GSQT = TG5 + M2 2.b1 x TG5 + b2 x M2 SAS Proc-Mix

TG5 & M2 Allele Frequencies

Marbling Score Analyses

% Choice Analyses

Conclusions: GeneStar Quality Hereford data Very low favorable allele frequencies No power to detect effect of markers

Charolais-Angus Data Marbling Score Non-significant estimate In direction of documentation provided by Bovigen % Choice GSQT star = 6.2%, p<.06 TG5 star > M2 star Conclusions: GeneStar Quality