PROJECT OVERVIEW

Outline  The RosBREED Project  The Need for RosBREED  Major Activities  RosBREED is Underway

The RosBREED Project

CROPS of the ROSACEAE FAMILY We’re In It Together

The RosBREED Project Vision

The RosBREED Project Mission

DNA information Socio-economics information The RosBREED Approach

1)Enhance new cultivar adoption, enlarge market potential, increase consumption – socio-economics knowledge of trait values 2) Establish sustainable infrastructure for MAB in Rosaceae – MAB Pipeline components 3) Integrate breeding and genomics information into breeding – breeding information management system 4) Conduct MAB in demo breeding programs, fruit quality – MAB Pipeline implementation 5) Enhance sustainability of cultivar development – MAB tech transfer to breeders, engage other stakeholders Project Objectives

PI: Amy Iezzoni, M ICHIGAN S TATE U NIVERSITY 4 years, S EP 2009 – A UG 2013 Funded by SCRI (USDA-NIFA’s Specialty Crop Research Initiative) $14.4 M ($7.2 M SCRI, $7.2 in-kind Partners) The RosBREED Project

Centered on breeders & breeding programs U.S.-wide and international collaboration 10 Teams (S OCIO -E CONOMICS, I NDUSTRY, B REEDING, P EDIGREE -B ASED A NALYSIS, B REEDING I NFORMATION M ANAGEMENT S YSTEM, G ENOMICS, G ENETICS, M ARKER -A SSISTED B REEDING P IPELINE, E XTENSION, A DMINISTRATION ) 32 members of 3 Advisory Panels (S TAKEHOLDER, E XTENSION, S CIENTIFIC ) The RosBREED Project

Breeding Program Locations

Amy Iezzoni Michigan State Univ. Nnadozie Oraguzie Washington State Univ. RosBREED’s Demonstration Breeders

Susan Brown Cornell Univ. Jim Luby Univ. Minnesota Kate Evans Washington State Univ. RosBREED’s Demonstration Breeders

Jim Hancock, strawberry breeder Tom Davis Univ. New Hampshire Chad Finn Oregon State Univ. RosBREED’s Demonstration Breeders

John Clark Univ. Arkansas Ksenija Gasic Clemson Univ. Tom Gradziel UC Davis David Byrne Texas A&M RosBREED’s Demonstration Breeders

Plant Research Intl., The Netherlands East Malling Research, United Kingdom INRA – Bordeaux, Avignon & Angers, France CRA-FRU Rome, Italy Andres Bello University, Chile ARC, South Africa Plant & Food Research, New Zealand RosBREED participant location International Partners

Fred Cook Rick Harrison Chrislyn Particka Gary van Sickle Peter Hirst Chris Watkins David Karp Bert Abbott Robin Buell Susan Gardiner Lailing Cheng Phil Simon Carolyn Ross Advisory Panel Color Key Industry Extension Scientific Jim Allen Chalmers Carr III Bill Dodd Bruce Grim Philip Korson Jessica Goldberger Mercy Olmstead Jamie Sherman Pere Arús Joe Avari Robert Curtis Henry Bierlink Ron Perry Clark Seavert Brian Sparks Fred Bliss Phil Baugher Tom Stokes Kevin Moffitt Systems Approach, Three Advisory Panels

The Need for RosBREED

The Chasm

Bridging the Chasm

DNA information Decision support Bridging the Chasm Genomics Resources More efficient development of new cultivars SE info

TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GENOME SCANS GDR, COMPARATIVE GENOMICS New QTLs discovered PBA: crop - wide PBA: breeding program Breeding markers Screening services Marker priorities Crossing scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT Socio-economics information DNA information Efficient routine breeding operations Better fruit! STANDARDIZED PHENOTYPING GERMPLASM CHOICE MAB PIPELINING Overview of Activities Seedling selection scheme

New QTLs discovered PBA: crop - wide PBA: breeding program Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit MAB PIPELINING GENOME SCANS STANDARDIZED PHENOTYPING GERMPLASM CHOICE TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS The Goal: Routine MAB BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

Major Activities

GENOME SCANS New QTLs discovered PBA: crop - wide PBA: breeding program Breeding markers Screening services Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE MAB PIPELINING GDR, COMPARATIVE GENOMICS TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT Marker priorities Socio-economics BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

GENOME SCANS New QTLs discovered Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Germplasm & Phenotyping PBA: crop - wide PBA: breeding program STANDARDIZED PHENOTYPING GERMPLASM CHOICE BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

New QTLs discovered Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Genome Scans GENOME SCANS PBA: crop - wide PBA: breeding program BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

GENOME SCANS Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS QTL Discovery New QTLs discovered PBA: crop - wide PBA: breeding program DNA information BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

GENOME SCANS New QTLs discovered PBA: crop-wide PBA: breeding program Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Fast-tracked Pipelining MAB PIPELINING Promising targets BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

PBA: crop - wide PBA: breeding program Breeding markers Screening services Marker priorities Crossing scheme Trial MASS GENOME SCANS New QTLs discovered Socio-economics information DNA information Better fruit GERMPLASM CHOICE MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Breeders’ Toolbox Efficient routine breeding operations STANDARDIZED PHENOTYPING MAB PIPELINING BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

New QTLs discovered PBA: crop - wide PBA: breeding program Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit MAB PIPELINING GENOME SCANS STANDARDIZED PHENOTYPING GERMPLASM CHOICE TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Technology Transfer BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GENOME SCANS GDR, COMPARATIVE GENOMICS New QTLs discovered PBA: crop - wide Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE Seedling selection scheme MAB PIPELINING News & Events BREEDERS’ TOOLBOX DEVELOPMENT

RosBREED is Underway

Meetings RosBREED I First Annual Meeting, 7-8 Jan 2010 RosBREED II Annual Advisory Panel Meeting, 13 Jan 2011

Website: Various information & resources

Acknowledgements This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture

MSU Amy Iezzoni (PD) Jim Hancock Dechun Wang Cholani Weebadde WSU Cameron Peace Dorrie Main Kate Evans Karina Gallardo Raymond Jussaume Vicki McCracken Nnadozie Oraguzie Mykel Taylor Cornell Susan Brown Kenong Xu Clemson Ksenija Gasic Gregory Reighard Texas A&M Dave Byrne Univ. of CA-Davis Tom Gradziel Carlos Crisosto Univ. of New Hamp. Tom Davis Univ. of Minnesota Jim Luby Chengyan Yue Oregon State Univ. Alexandra Stone Plant Research Intl, Netherlands Eric van de Weg Marco Bink USDA-ARS Nahla Bassil Gennaro Fazio Chad Finn Univ. of Arkansas John Clark