The structure and function of Macromolecules Chapter 5 notes The structure and function of Macromolecules

Concept 5.1 Polymer: a long molecule consisting of similar or identical building blocks - like a train with many cars - monomers: repeating units that are the building blocks Diversity of life is based around 40 or 50 polymers

Concept 5.1 Monomers are connected together by dehydration synthesis (anabolic) - covalent bond w/ the loss of H2O Polymers are broken down by hydrolysis (catabolic) - reverse rxn. of dehydration synthesis - “break with water”

Concept 5.1 1 2 3 H HO H HO Unlinked monomer Short polymer Dehydration removes a water molecule, forming a new bond H2O HO 1 2 3 4 H Longer polymer (a) Dehydration reaction in the synthesis of a polymer

Concept 5.1 HO H2O H 3 2 1 4 Hydrolysis adds a water molecule, breaking a bond HO H2O H 3 2 1 4

Concept 5.2 Carbohydrates: include sugars and polymers Monosaccharides (monos = single; sacchar = sugar) - molecular formula is CH2O - ex. Glucose (C6H12O6) - most sugars are rings when aqueous

Concept 5.2 (a) Linear and ring forms (b) Abbreviated ring structure

Concept 5.2 Disaccharide: two monosaccharides joined by a glycosidic linkage - formed by dehydration synthesis - glucose + glucose = maltose + H2O - glucose + fructose = sucrose + H2O

Concept 5.2 (b) Dehydration reaction in the synthesis of sucrose Glucose Fructose Sucrose Maltose (a) Dehydration reaction in the synthesis of maltose

Concept 5.2 Polysaccharides: polymers w/ few hundred to a few thousand monomers -fcn. of a polysaccharide is determined by monomers and positions of glycosidic linkages

Concept 5.2 Storage polysaccharides - starch: found in plants; consists only of glucose monomers (1-4 linkage); how plants store glucose (chloroplasts) - glycogen: polymer of glucose found in animals; stored in liver and muscle cells

Concept 5.2 Structural polysaccharides - cellulose: major component of plant cell walls - note: 2 ring structures of glucose (alpha (a) and beta (b)) - cellulose is composed of all b glucose - cellulose = “insoluble fiber”

Concept 5.2  Glucose  Glucose (a)  and  glucose ring structures

Concept 5.2

Concept 5.2 Cell walls microfibrils in a plant cell wall Microfibril Glucose monomer Cellulose molecules Microfibril microfibrils in a plant cell wall 0.5 µm 10 µm Cell walls

Carbohydrates Structural polysaccharides (cntd.) - chitin: carbohydrate used by arthropods to build exoskeletons - also used to make decomposable surgical thread

Concept 5.2 The structure of the chitin monomer. (a) (b) (c) Chitin forms the exoskeleton of arthropods. Chitin is used to make a strong and flexible surgical thread.

Concept 5.3 Lipids: little or no affinity for water; consist mostly of hydrocarbons - 3 families: fats, phospholipids, steroids Fat: composed of 2 parts - glycerol: 3 carbon alcohol w/ hydroxyl - fatty acids: long carbon skeletons w/ carboxyl groups

Concept 5.3 Fatty acid (palmitic acid) Glycerol (a) Dehydration reaction in the synthesis of a fat

Concept 5.3 Fatty acids can vary in length and in the number and location of double bonds - “saturated”: no double bonds; most animal fats; solid at room temp. - “unsaturated”: has one or more double bonds which removes H atoms; plants and fish; liquid at room temp.

Concept 5.3 Structural formula of a saturated fat molecule Stearic acid, a saturated fatty acid

Concept 5.3 unsaturated fatty acid Oleic acid, an cis double Structural formula of an unsaturated fat molecule Oleic acid, an unsaturated fatty acid cis double bond causes bending

Concept 5.3 The major fcn. of fats is energy storage. - 1g of fat stores more than twice as much energy as 1g of a polysaccharide - mammals stock food reserves in adipose cells

Concept 5.3 Phospholipids: similar to fats but have only 2 fatty acid tails; 3rd hydroxyl group joins to a phosphate group - show ambivalent behavior to water - head= polar (hydrophilic); tail= nonpolar (hydrophobic) - arranged in a bilayer, or double layer

Concept 5.3 (b) Space-filling model (a) (c) Structural formula Phospholipid symbol Fatty acids Hydrophilic head Hydrophobic tails Choline Phosphate Glycerol Hydrophobic tails Hydrophilic head

Concept 5.3 Steroids: lipids with a carbon skeleton consisting of 4 fused rings - differ in functional groups attached to rings - Cholesterol: found in animal cell membranes; precursor for other steroids

Concept 5.3

Concept 5.4 Proteins: account for more than 50% of the dry weight of most cells - used for structural support, storage, transport, signaling, movement, and defense

Concept 5.4 Proteins are polymers constructed from the same set of 20 amino acids - called polypeptides - consist of one or more polypeptides folded and coiled into specific conformations

Concept 5.4 Amino acids are the building blocks of proteins - a carbon is bonded to an animo group, a carboxyl group, a hydrogen atom, and a variable (R) - grouped according to side chains (nonpolar, polar, acidic, basic)

Concept 5.4

Concept 5.4

Concept 5.4 Amino acids are bonded together by a peptide bond - carboxyl group of one amino acid connects w/ the amino group of another (dehydration synthesis)

Concept 5.4

Concept 5.4 Four levels of protein structure - primary structure: unique sequence of amino acids - even a slight change can affect a proteins conformation and ability to function - ex. Sickle-cell disease

Concept 5.4

Concept 5.4 - secondary structure: coils or folds that are a result of hydrogen bonds at regular intervals - a helix: delicate coil held together by hydrogen bonding between every fourth amino acid - b pleated sheets: two or more regions lie parallel to each other

Concept 5.4

Concept 5.4 - tertiary structure: irregular contortions from interactions between side chains (R groups) - hydrophobic interactions: nonpolar side chains cluster in the core, away from water - van der Waals interactions help hold them together

Concept 5.4 - disulfide bridges: covalent bond between two cysteine monomers (have sulfhydryl groups) - ionic bonds and hydrogen bonds also contribute

Concept 5.4

Concept 5.4 - quaternary structure: overall protein structure resulting from combining of multiple subunits The unique conformation endows each protein with a specific function

Concept 5.4

Concept 5.4

Concept 5.4 The unique conformation endows each protein with a specific function - denaturation: protein unravels and losses its conformation - pH, [salt], temperature

Concept 5.5 Compounds that are responsible for determining the amino acid sequence of a polypeptide. Two types of nucleic acids - deoxyribonucleic acid (DNA) - ribonucleic acid (RNA) Flow of genetic information: DNA  RNA  Protein

Concept 5.5 mRNA Synthesis of mRNA in the nucleus DNA NUCLEUS CYTOPLASM Movement of mRNA into cytoplasm via nuclear pore Ribosome Amino acids Polypeptide Synthesis of protein 1 2 3

Concept 5.5 Nucleotides are the monomers (building blocks) of nucleic acids -nucleotide = nitrogenous base + pentose (5-carbon sugar) + phosphate

(a) Polynucleotide, or nucleic acid (c) Nucleoside components: sugars Concept 5.5 5 end Nucleoside Nitrogenous base Phosphate group Sugar (pentose) (b) Nucleotide (a) Polynucleotide, or nucleic acid 3 end 3C 5C Nitrogenous bases Pyrimidines Cytosine (C) Thymine (T, in DNA) Uracil (U, in RNA) Purines Adenine (A) Guanine (G) Sugars Deoxyribose (in DNA) Ribose (in RNA) (c) Nucleoside components: sugars

Concept 5.5 Two families of nitrogenous bases: - pyrimidines: single ring; cytosine (C), thymine (T), and Uracil (U) - purines: double ring; adenine (A), and guanine (G)

Concept 5.5 Difference between DNA and RNA is in the sugar. DNA lacks an oxygen atom attached to its number 2 carbon Polynucleotide: nucleotides are joined by phosphodiester linkage

Concept 5.5 DNA molecules have two polynucleotides that form a double helix. - Watson and Crick (1953) - A binds to T; C binds to G; forms two complementary strands