Introduction to the Microscope

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Introduction to the Microscope

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Presentation transcript:

Introduction to the Microscope What is it? Care Parts Focusing

The Light Microscope Compound microscope uses compound lenses and light to magnify objects The lenses bend or refract the light, which makes the object beneath them appear closer Hans and Zacharias Janssen are credited with creating the first microscopes Robert Hooke and Anton van Leeuwenhoek were pioneers in early microscope development

Microscope Care Always carry with 2 hands (one hand on the arm, one on the base) Only use lens paper for cleaning Do not force knobs Keep cords away from sinks due risk of electrical shock

Microscope Parts Eyepiece Body Tube Revolving Nosepiece Arm Objective Lens Stage Stage Clips Coarse Focus Diaphragm Fine Focus Light Base

Using the Microscope Place the Slide on the Microscope Use Stage Clips Click Nosepiece to the lowest (scanning) objective Look into the Eyepiece and adjust the slide until object is centered Use the Coarse Adjustment Knob to bring into focus When observations are completed (at this magnification,) proceed to adjust to Low Power.

Using High Power When High Power is needed, make sure the object is centered and focused at scanning and low power Click the nosepiece to the high power objective (largest) lens Do NOT use the Coarse Focusing Knob Use the Fine Focus Knob to bring the slide into focus Never remove a slide while under high power

Magnification Your microscope has 3 magnifications: Scanning, Low and High objective lens Each objective lens will have written the magnification. In addition to this, the ocular lens (eyepiece) has a magnification The total magnification is the eyepiece times the objective lens

Troubleshooting Q: Image is too dark! A: Adjust the diaphragm, make sure your light is on Q: There's a spot in my viewing field, even when I move the slide the spot stays in the same place! A: Your lens is dirty. Use lens paper, and only lens paper to carefully clean the objective and ocular lens Q: I can't see anything under high power! A: Remember the steps, if you can't focus under scanning and then low power, you won't be able to focus anything under high power Q: Only half of my viewing field is lit, it looks like there's a half-moon in there! A: You probably don't have your objective fully clicked into place