MICROSCOPE.  Microscope is a tool that is used to observed microscopic objects that cannot be seen with the naked eyes.  A light microscope works by.

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Presentation transcript:

MICROSCOPE

 Microscope is a tool that is used to observed microscopic objects that cannot be seen with the naked eyes.  A light microscope works by means of centralizing light caught by our eyes to form a magnified image of an object.

Objectives  To know parts and their functions of a microscope To use a microscope correctly To use a microscope correctly

Stereo microscope Light microscope A stereo microscope is often used for lower-power magnification on large subjects

Electron Microscope Electron microscopes, which use beams of electrons instead of light, are designed for very high magnification usage. Electrons, which have a much smaller wavelength than visible light, allow a much higher resolution.

Mirror Objective lens Hole Diaphragm Eyepiece Body tube Revolver Coarse Adjustment Stage Stage Clips Base Arm Fine Adjustment Microscope Parts

Body tube Base Stage Clips Revolver Arm

Parts of Microscope and their function  Eyepiece (Lensa Okuler): to magnify image you look through  Coarse adjusment (Pemutar Kasar): focuses the image under low power  Body tube (Tabung): connect the eyepiece to the revolving nose piece  Fine adjustment (Pemutar Halus): sharpens the image under high and low magnification

 Revolver (Pemutar Lensa): holds and turns the objectives into viewing position  Low-power objective Lens (Lensa Objektif Pembesaran Lemah): contains the lens with lower power magnification  High-power objective Lens (Lensa Objektif Pembesaran Kuat): contains the lens with high (the most) magnification  Arm (Lengan Mikroskop): support the body tube.

SSSStage (Meja Benda): platform used to support the microscope slide SSSStage clips (Penjepit): hold the microscope slide in place DDDDiaphragm (Diafragma): regulates the amount of light entering the body tube MMMMirror (Cermin): allows light to go upward through the diaphragm, the specimen, and the lenses BBBBase (Kaki Mikroskop): provides support for the microscope

How to figure out the Magnification? Low Power 10 X 10 X 100 X Eyepiece Objective Lens Magnification High Power 10 X 45 X 450 X

Working with your microscope ! 1. P lace the microscope on flat surface. The arm should be toward you. 2. L ook through the eyepiece. Open the diaphragm. 3. A djust the mirror until you can see the most light coming through the tube. Seven easy steps for beginners !

4. P lace the slide so that the material to be observed is in the middle of the hole on the stage. 5. T urn the nosepiece to the low- or medium- power objective- never start to the high-power. 6. T urn the coarse adjustment until the objective goes as far as it can without touching the slide. 7. F inally, look through the eyepiece and sharpens the image by turning the fine adjustment.

Attentions !! 1.Always carry the microscope by holding the arm with one hand and supporting the base with the other hand. 2. Do not touch the lenses with your finger. 3. Always focus first with lower-power objective. 4. Do not use coarse adjustment when the high power objective is in place.

Once you have examined the specimen under low power, try this:  fiddle with the mirror. Sometimes you can change the light and see the specimen in a different way.  as you look through the eyepiece, try moving the slide to see different parts of the specimen. This takes practice.  try a high-power objective, but expect the light to be dimmer. You will probably have to change the focus a bit.

Position of Object Glass and Cover Glass