Parts of the microscope
2 Compound Light Microscope Used to observe small objects Magnifies images up to 2000X their size
3 Different parts of a microscope copyright cmassengale
SCI.9-12.B [Indicator] - Use appropriate laboratory apparatuses, technology, and techniques safely and accurately when conducting a scientific investigation
1.Optic or body tube – connects eyepiece to objective lens 2.Revolving nosepiece – turns objectives Type of objectives – they all magnify at different strengths 3. Scanning (the shortest one) -- magnifies 4x B(4). Low power objective – magnifies 10x C(5). High power objective (longest one) -- magnifies 40x
M(6). Stage clips – holds slide in place J(7). Diaphragm – controls how much light is on slide – 2 types are a. wheel and b. lever K(8). Light A(9)Eyepiece – look through it and magnifies 10 x H (10)Arm – carry microscope with D (11)Stage – holds slide or object being looked at
F(12). Coarse focus – sharpens image by moving stage G(13). Fine focus knob – sharpens image, see depth and adjusts for differences in eyesight E(14). Base – supports microscope
Total magnification – how many times what you are looking at is magnified T.M. = eyepiece x objective Scanning x 4 = 40 Low power – 10 x 10 = 100 High power – 10 x 40 = 400 Oil immersion – 10 x 100 = 1000
SCI.9-12.B [Indicator] - Use appropriate laboratory apparatuses, technology, and techniques safely and accurately when conducting a scientific investigation
Resolution is how clear an image is. As magnification goes up, resolution goes down. We say they are inversely related.
12 Eyepiece Fine adjustment Arm Stage Condenser control knob Base Body tube Coarse adjustment Iris diaphragm Revolving nosepiece Objective Clip Condenser Mirror
Observing Cells Light microscope – Can observe living cells in true color – Magnification of up to ~1000x – Resolution ~ 0.2 microns – 0.5 microns
Observing Cells Electron Microscopes – Preparation needed kills the cells – Images are black and white – may be colorized – Magnifcation up to ~100,000 Transmission electron microscope (TEM) – 2-D image shows inside Scanning electron microscope (SEM) – 3-D image shows outside
SEM TEM