450 400 350 300 250 200 150 100 50 0 Dau c 1.02/Ubiquitin C C A C A C A C A 0 6 12 24 72 Hours post infection Dau c 1.01/Ubiquitin A B Figure S 1 A and.

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Dau c 1.02/Ubiquitin C C A C A C A C A Hours post infection Dau c 1.01/Ubiquitin A B Figure S 1 A and B C: control A: A. radicina

Fig. S 1: Accumulation of Dau c 1 mRNAs (synonymic DcPR10) in field grown carrots upon challenge with the fungal pathogen Alternaria radicina. Slices (0.5 cm thick) from the middle part of 4-month-old fresh roots of the cultivar Rodelika were placed on moist filter paper in a petridish. The slices were inoculated with 105 conidia (10 µl aqueous suspension) and incubated at RT in the dark. Transcripts of Dau c 1.01 (A) and Dau c 1.02 (B) were determined by qPCR at the indicated time points. Values are given relative the ubiquitin RNA.

Figure S 2 Fig. S 2: Schematic representation of the T-DNA region inserted into plant transformation RNAi vector pK7GWIWG2(II). A 253 nt fragment of Dau c 1.01 (A) and 267 nt fragment of Dau c 1.02 (B) were inserted separately in sense and anti sense orientation into vector pK7GWIWG2(II), resulting in plasmids pK7GW_Dau c 1.01_RNAi and pK7GW_Dau c 1.02_RNAi.

No. professio n Past medical history: Further allergies (other than carrot) clinical symptom s (carrot) Oral food challenge (carrot) Routine Skin Prick Test ImmunoCAP [kU A /L] (CAP class)experimental ImmunoCAP [kU A /L] (CAP class) carrot birch pollen (Allergo- pharma) carrotrBet v 1rBet v 2Bromelin rDau c rDau c rDau c 4 1 travel agent Seasonal allergic rhinoconjunctivitis (march to june) with beginning asthmatic symptoms. Diagnosed with type I allergies to birch-, weed- and grass-pollen, moulds. OAS and contact urticaria to apple. OAS and contact urticaria pos 0.10 (0)6.62 (3)0.01 (0)0.03 (0)0.00 (0)2.04 (2)0.01 (0) 2 medical secretary Seasonal allergic rhinoconjunctivitis (february to september). Diagnosed with type I allergies to birch- and grass-pollen, moulds, OAS to apple and orange. OASpos 0.07 (0)43.1 (4)7.46 (3)0.28 (0)5.25 (3)4.32 (3)2.11 (2) 3 consulting engineer Seasonal allergic rhinoconjunctivitis (march to may). Diagnosed with type I allergies to birch-pollen. Severe type I allergy to bee and yellow jacket venom (anaphylactic shock reaction) OASpos 2.82 (2)35.9 (4)0.03 (0)0.88 (2)3.7 (3)4.32 (3)0.09 (0) 4 field engineer Seasonal allergic rhinoconjunctivitis (march to june) with beginning asthmatic symptoms. Diagnosed with type I allergy to birch-pollen. OAS to apple, celery, hazelnut, peanut. type I allergies (allergic asthma) to cat-, horse- and dog dander. Severe type I allergy to yellow jacket venom (anaphylactic shock reaction) OASpos 5.20 (3)55.8 (5)2.7 (2)0.18 (0)10.5 (3)4.9 (3)1.48 (2) 5physician Seasonal allergic rhinoconjunctivitis (march to june) with beginning asthmatic symptoms. Diagnosed with type I allergy to birch-pollen. OASpos 2.20 (2)9.28 (3)1.30 (2)0.02 (0)2.53 (2)1.55 (2)0.88 (2) 6physician Seasonal allergic rhinoconjunctivitis (march to june) with beginning asthmatic symptoms. Diagnosed with type I allergy to birch-pollen. OAS to apple, hazelnut and cherries. OASpos nd0.62 (1)5.35 (3)0.00 (0)0.08 (0)0.64 (1)0.91 (2)0.05 (0) 7 university student Seasonal allergic rhinoconjunctivitis (february to june) with beginning asthmatic symptoms. Diagnosed with type I allergy to birch-pollen. Atopic dermatitis. OASpos nd1.43 (2)>100 (6)0.08 (0)0.21 (0)12 (3)0.77 (2)0.09 (0) 8physician Seasonal allergic rhinoconjunctivitis (march to june). With beginning asmatic symptoms. Diagnosed with type I allergies to birch-pollen. OASpos 1.05 (2)10.5 (3)0.00 (0)0.07 (0)0.69 (1)1.06 (2)0.04 (0) 9 Interior decorator Seasonal allergic rhinoconjunctivitis (february to may). Diagnosed with type I allergies to birch-pollen. OAS to apple, pear, hazelnut and tomato. OASpos 24.0 (4)>100 (6)0.13 (0)2.90 (2)28.4 (4)16.6 (3)0.54 (1) 10physician Seasonal allergic rhinoconjunctivitis (february to april) Diagnosed with type I allergy to birch- and mugwort-pollen. OASndpos 8.16 (3)0.06 (0)0.14 (0)0.36 (1)4.86 (3)14.4 (3)0.18 (0) TABLE S 1

Table S 1: Clinical and serologic characterization of patients with carrot allergy. (OAS = oral allergy syndrome. ImmunoCAP classes and kilounits per liter (kUA/L) are related as follows: 0.7 to ≤3.5 kUA/L = class 2; >3.5 to ≤17.5 kUA/L = class 3; >17.5 to ≤50 kUA/L = class 4; >50 to ≤100 kUA/L = class 5; >100 kUA/L = class 6.).