PRESENTATION II NAME By Christine Harvey Dihydrofolate Reductase Beta lactmase p300 Macrophage Infectivity Potentiator.

Slides:

Advertisements

Similar presentations

Understanding the complexity of Protein Function

Advertisements

Jeopardy Cell Wall Plasma Membrane DNA Anti- metabolite Protein Translation Q $100 Q $200 Q $300 Q $400 Q $500 Q $100 Q $200 Q $300 Q $400 Q $500 Final.

Enzyme Mechanisms.

Methods for detection β-lactamases Sarah Alharbi.

Novel Dihydrofolate Reductase Inhibitors. Structure- Based versus Diversity-Based Design and High- Throughput Synthesis and Screening Pierre C. Wyss, Paul.

1 Antimicrobial Therapy Chemotherapy: any treatment of patient with chemicals to treat a condition. –Now word associated with cancer treatment –Our focus.

Chapter Twenty One Enzymes and Vitamins. Ch 21 | # 2 of 47 Catalysts for biological reactions Proteins Lower the activation energy Increase the rate of.

Dihydrofolate Reductase (DHFR) Ruixiao ‘Ray’ Gao Department of chemistry Illinois state university 10/30/2014.

1 Antimicrobial Therapy Chemotherapy: any treatment of patient with chemicals to treat a condition. –Now word associated with cancer treatment –Our focus.

Organic Chemistry 4 th Edition Paula Yurkanis Bruice Chapter 25 The Organic Mechanisms of the Coenzymes Metabolism Irene Lee Case Western Reserve University.

Antibiotics Biotechnology II. Univ S. Carolina Antibiotics Disrupt Cell Wall Synthesis, Protein Synthesis, Nucleic Acid Synthesis and Metabolism.

Enzymes. Definition of an enzyme Enzymeprotein Enzyme is protein catalystincrease the rate of reactions catalyst (i.e. increase the rate of reactions)

Section 2.5: Enzymes Biology.

Cell signaling Lecture 8. Transforming growth factor (TGF β) Receptors/Smad pathway BMP7 TGF β1, TGF β2, TGF β3 Dpp Inhibins Activins TGF β receptors.

1 SURVEY OF BIOCHEMISTRY Transcription and Translation.

PHAR2811 Dale’s lecture 3 Genome Structure COMMONWEALTH OF AUSTRALIA Copyright Regulation WARNING This material has been reproduced and communicated to.

Chapter 22 Biosynthesis of amino acids, nucleotide 1. Source of nitrogen. 2. Source of carbon. 3. De novo and salvage pathways. 4. Ways to balance the.

MPP OUTSIDE REQUEST FORM Reviewed 07/27/11 Requestor(s): Dean Appling Institution: U. Texas-Austin Request Title: Human mitochondrial proteins MTHFD1L.

Chemistry 20 Chapter 15 Enzymes.

Protein synthesis  30.4 Ribosome Structure and Assembly  30.5 Mechanics of Protein Synthesis  30.4 Ribosome Structure and Assembly  30.5 Mechanics.

1 Chapter 3: Protein ZHOU Yong Department of Biology Xinjiang Medical University.

Fig The Elongation Cycle (in prokaryotes).

Penicillin and Cephalosprin: Beta- Lactam Antibiotics and Other Inhibitors of Cell Wall Synthesis by Dena Nguyen

“Emerging infections, the re-emergence of infectious diseases previously considered to be under control, and the alarming trend of antibiotic resistance….require.

ATP Immediate source of energy that drives cellular work Adenosine triphosphate Nucleotide with unstable phosphate bonds Phosphate bonds easily hydrolyzed.

Regulation of Gene Expression

Chapter 15 Enzymes. Enzymes Ribbon diagram of cytochrome c oxidase, the enzyme that directly uses oxygen during respiration.

Valyl-tRNA Synthetase Josh Jarodsky

Chp Chemistry 121 Winter 2009 LA Tech Introduction to Organic Chemistry and Biochemistry Instructor Dr. Upali Siriwardane (Ph.D. Ohio State)

1 SURVEY OF BIOCHEMISTRY Enzyme Catalysis. 2 General Properties of Enzymes High reaction rates –10 6 to times faster than uncatalyzed reaction Mild.

Enzymes Biomolecules that catalyze chemical reactions - Increase reaction rates - Specific Oxidoreductases – catalyze redox reactions Transferases – transfer.

Hein * Best * Pattison * Arena

Genetic Engineering BSC 1010L Transformation of E. coli with Jellyfish GFP.

Valyl-tRNA Synthetase Josh Jarodsky

The Crystal Structure of Helicobacter pylori Cysteine-rich Protein B Reveals a Novel Fold for a Penicillin-binding Protein Lucas Lu¨ thy, Markus G. Gru¨

Mechanism of alcohol dehydrogenase

and Bacterial Resistance

What is so memorable about CREBBP? (RSTS; RTS, CBP CBP/MOZ FUSION GENE, CREB-binding protein)

X-ray crystallography – an overview (based on Bernie Brown’s talk, Dept. of Chemistry, WFU) Protein is crystallized (sometimes low-gravity atmosphere is.

Dihydrofolate Reductase (DHFR)

How Enzymes Work Pratt & Cornely Ch 6.

Aspirin Inhibits Cycloxygenase

Legionella pneumophila Ubiquitous in soil and water causative agent of Legionnaire’s disease Only recognized within last 20 years.

Objectives Understand the basic principles of antimicrobial chemotherapy Overview of the following as major bacterial targets for antibiotics: Examples.

Biotechnology Made up of 3 technologies: Bioprocess technology: when microorganisms are provided with nutrients and advantageous conditions, they perform.

How Enzymes Work Pratt & Cornely Ch 6.

Chapter 24 The Organic Chemistry of the Coenzymes,

Sample Problem 20.1 The Enzyme Active Site

Bacterial Targets for Antimicrobial Therapy

MYC From: Wikipedia Nicholas Britt (Wikipedia, 2008)

Kostelecky et al. 2006, Journal of Bacteriology The Crystal Structure of the Zinc Phosphodiesterase from Escherichia coli Provides Insight into Function.

Enzymes Enzymes as Biological Catalysts

An In-Depth Look at the GES Family of Enzymes

Enzymes Enzymes can speed up a chemical reaction without being altered. Enzymes are biological catalysts. Each acts on a specific substance. The specificity.

Retinoblastoma and the Rb1 Gene

Sustaining Proliferative Signaling and Evading Growth Suppressors

Control of blood proteins by functional disulfide bonds

Yuvaniyama et al., 2003, Nature Structural Biology

Chapter 20 Antibacterial Agents

Chemotherapeutic Medicine

Epigenetics modification

Linking transcriptional mediators via the GACKIX domain super family

DNA-Induced Switch from Independent to Sequential dTTP Hydrolysis in the Bacteriophage T7 DNA Helicase Donald J. Crampton, Sourav Mukherjee, Charles.

Factors that Mediate and Modulate Androgen Action

Volume 21, Issue 7, Pages (July 2014)

Deactivation of Carbapenem-Hydrolyzing Class D β-Lactamases (CHDLs) Through the Modification of C6-Substituted Penicillin Derivatives Alyssa Bangrazi,

Thymidylate synthase as an oncogene?

Confirmation of candidates from WT1 binding site screen.

성균관대학교 약학과 김연수

Presentation transcript:

PRESENTATION II NAME By Christine Harvey Dihydrofolate Reductase Beta lactmase p300 Macrophage Infectivity Potentiator

Dihydrofolate Reductase in Escherichia coli

Class: oxidoreductase Reduces dihydrofolate (DHF) to tetrahydrofolate (THF) THF is a precursor to amino acids, purine and thymidylate. DHFR is imperative to cell life

Function Source: Schweitzer, Barry I., et al. The FASEB Journal Vol 4, “Dihydrofolate reductase as a therapeutic target.” May 1990.

Mechanism

Structure

Binding Pocket

MOVIE

Sequence Alignment

Phylogenetic Tree

Beta-Lactamase in Escherichia coli

Class: Hydrolase Beta Lactamase is produce by bacteria after being exposed to beta lactam antibiotics Responsible for beta lactam antibiotic resistance

Penicillin Contains beta lactam ring

Mechanism

21

p300 In Homo Sapiens

Class: Transferase p300 transfers an acetyl group from acetyl coenzyme A to an active site lysine Transcriptional activation occurs upon acetyl transfer

Title p300 is responsible for cell growth before and after birth Cancer suppressor

Domains Taz2 KIX Bromodomain HAT Zinc Fingers (3) Taz2 Creb

Histone Acetyl Transferase Active site tyrosine acts as nucleophile Source:

Docking

39

Macrophage Infectivity Potentiator in Legionella pneumophila

Legionella pneumophila gram negative bacteria Causes Legionnaires’ disease Carried in aerosols from man-made water systems

MIP Class: Isomerase Catalyzes the isomeration of the N- terminal on proline Cause of virulence in Legionnaires’ disease Located on outer membrane protein 25 kDa

Crystalization Solution: 100 mM MES (pH 6.1–6.5), 15–20% (w/v) PEG 8000 and 500 mM zinc acetate. Protein concentration was 10 mg ml–1in 20 mM HEPES, pH 7.0 Crystals were grown for 2-3 weeks at 288K X-ray data was collected at 100K in a nitrogen gas stream (dried paraffin oil) at 2.4 Angstrom resolution Multiwavelength anomalous dispersion (MAD) with zinc was used for phasing

44 Source: Riboldi-Tunnicliffe, Alan et al., Nature Structural Biology, vol.8: 9. “Crystal structure of Mip, a prolylisomerase from Legionella pneumophila.” September 2001.

Mechanism Peptidyl-prolyl cis-trans isomerases’ (PPIases) mechanism is not currently know Source: Park, Steven T., et al. Journal of Biological Chemistry. Vol. 267 No.5, “PPIase Catalysis by Human Fk506-binding Protein Proceeds Through a Conformational Twist Mechanism.” February 1992.

Source: Riboldi-Tunnicliffe, Alan et al., Nature Structural Biology, vol.8: 9. “Crystal structure of Mip, a prolylisomerase from Legionella pneumophila.” September 2001.

49

51

52

53 Questions?