Microscopy Looking at the small stuff. Microscopes  “micro” means : Small  “scope” means: Watch/ look  It was actually developed first as entertainment.

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Presentation transcript:

Microscopy Looking at the small stuff

Microscopes  “micro” means : Small  “scope” means: Watch/ look  It was actually developed first as entertainment for the rich

Light Microscope  Focuses visible light rays  Can be used on live or dead specimens  Dyes and fluorescent markers help for viewing  Magnify up to 1000X on the ones we have  Examples: Compound light microscope Confocal microscope

Light Microscope

Light Micrographs Plant magnified Human chromosomes 

Confocal Microscope

Confocal micrograph Spirogyra crassa Maximum projection green: Actin. Rhodamin, Label: Phalloidin. Actin red: Chloroplasts. Autofluorescence Image acquired with the Leica TCS SP2

Electron Microscopy (EM)  Focus beam of electrons on the specimen  Specimens must be dead  Kinds Transmission Electron Microscope (TEM)  Cross-section of the material  Most detailed of internal of cell Scanning Electron Microscope (SEM)  Surface of the specimen  Shows texture, but less detail

TEM

SEM

Ant Pollen Grains

Red Blood Cells

Compare cardiac myocytes Maximum projection (overlay) of neonatal cardiac myocytes from rat. green: Myomesin, FITC red: Cadherin, Cy3 blue: DNA (nuclei), DRAQ5 Image acquired with the Leica TCS SL Compare to TEM of myoctes on the wall.

Keen eyes  Light microscopes will not show you the nucleus of a cell or something the size of a virus!  Confocal microscopy uses brightly colored markers  TEM is detailed, flat, and black and white  SEM is 3D, textured, and black and white

Cited pictures  Pollen grains (1000x magnification). Paul Fuqua unitedstreaming. 29 August  Blood cells, red; microscopic view. U.S. Army unitedstreaming. 29 August  medlib.med.utah.edu/WebPath/HEMEHTML/HEME001.h tml medlib.med.utah.edu/WebPath/HEMEHTML/HEME001.h tml   microscopy.com/WebSite/pictures.nsf/(ALLIDs)/23C65 31FD6B41E6BC1256A7D0053F8CA/$FILE/drosoegg_ap p_img_i_cl.jpg microscopy.com/WebSite/pictures.nsf/(ALLIDs)/23C65 31FD6B41E6BC1256A7D0053F8CA/$FILE/drosoegg_ap p_img_i_cl.jpg  microscopy.com/WebSite/pictures.nsf/(ALLIDs)/23C65 31FD6B41E6BC1256A7D0053F8CA/$FILE/drosoegg_ap p_img_i_cl.jpg

Cited Pictures  M/image/31formicidae40(extra).jpg M/image/31formicidae40(extra).jpg  machine.mine.nu/Mysterymoss/Micro3_DSCN648 6.jpg machine.mine.nu/Mysterymoss/Micro3_DSCN648 6.jpg    /confocal%20microscope.jpg /confocal%20microscope.jpg  osomes2.jpg osomes2.jpg  state.edu/~plantbio/osu_pcmb/images/Courses/P B101/plant_cell_tem.jpg