Microscope History and Development

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Presentation transcript:

Microscope History and Development Field of view and Magnification

Robert Hooke In 1665, the English physicist Robert Hooke looked at a sliver of cork through a microscope lens and noticed some "pores" or "cells" in it. Hooke was the first person to use the word "cell" to identify microscopic structures when he was describing cork.

Antique microscopes

Early Microscopes - Anton Van Leeuwenhoek The father of microscopy, Anton Van Leeuwenhoek of Holland (1632-1723). Anton Van Leeuwenhoek was the first to see and describe bacteria (1674), yeast plants, the teeming life in a drop of water, and the circulation of blood corpuscles in capillaries.

Robert Brown In 1820 was using a more advanced microscope with 2 lenses and was able to see that there were smaller parts within a cell He was the first to use the term “nucleus” to describe a round object in the center of the cell

The Cell Theory All living things are made up of one or more cells The cell is the functional unit of life.

Technological Advances in Microscopes

Compound Light Microscopes Uses light Has two lenses Magnification limited to 2000x (400x at LHHS)

Transmission Electron Microscope (TEM) Uses beams of electrons Magnification of 2 000 000x Has two limitations: Good only for thin specimens Only dead cells can be observed

Scanning Electron Microscope (SEM) Electrons are reflected from the surface of the specimen Produces a 3-d image Good for the thicker specimens Lacks the magnification and resolution of the transmission electron microscope

Magnification Magnification = Objective lens X Ocular lens (4x, 10x, 40x) (10x)

Calculating the size of a specimen binder

Calculating the size of a specimen Example under med. objective Object size = Size of field of view Number of objects across field of view Object size = 1.72 mm 14 Object size = 0.1 mm