Compound Microscope How to properly carry the microscope Two-dimensional images View internal structures.

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Presentation transcript:

Compound Microscope

How to properly carry the microscope Two-dimensional images View internal structures

With a binocular microscope, adjust oculars for both eyes!

Stereoscopic (Dissecting) Microscope Three-dimensional images View external structures

Stereoscopic close-up of finger

Microsurgery being performed with a stereoscope

Fluorescence Microscopy  Cells are stained with fluorescent dyes called fluorochromes.  Uses UV light as energy source.  Fluorochromes absorb UV light and emit visible light.  Produces green to yellow color

Digital Microscope System Automated system: Auto-focus, auto-aperture, auto- light……

Electron Microscopy  Uses a beam of electrons  Very high magnification of up to 100,000 X.  Transmission electron microscope (TEM): Two-dimensional images Two-dimensional images View internal structures View internal structures  Scanning electron microscope (SEM): Three-dimensional images Three-dimensional images View external structures View external structures

TEM and SEM Compared Scanning Electron Microscope Transmission Electron Microscope

Transmission Electron Microscopy (a) a virus and (b) a protozoan. E. coli

Scanning Electron Microscopy ParameciumAspergillus

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