Microscopes Light microscope Depth of field: small.

Slides:

Advertisements

Similar presentations

Lecture 11. Microscopy. Optical or light microscopy involves passing visible light transmitted through or reflected from the sample through a single or.

Advertisements

Microscopes. Purpose of scopes Magnification – make objects appear larger Resolution – keep the image clear.

Tools of a Biologist. Microscopes: number one tool of a biologist Micro=Scope= – Small-to view A microscope is an instrument used to view very small objects.

MICROSCOPES. Microscope Quiz Friday –Jan. 28 n Label parts of microscope n How to use (ex: use coarse knob to find object, adjust diaphragm for light)

Sections 4.1, 4.2 CELLS AND MICROSCOPES.  Microscopes allow us to identify organisms that cause food to spoil and cause disease.  1665 Robert Hooke-

USE AND CARE OF THE MICROSCOPE LECTURE 1. MICROSCOPY u Light Microscopy: any microscope that uses visible light to observe specimens u Compound Light.

©2001 Timothy G. Standish Psalm 119:99, I have more understanding than all my teachers: for thy testimonies are my meditation. 100 I understand more.

Compound Light Microscope

Microscopes The Discovery of Cells Quiz Number paper from 1-5 Identify the following pictures.

Microscopy Chapter 3 Biology Mr. Gilbertson. DISCOVERY OF CELL PRECEDED BY THE INVENTION OF THE MICROSCOPE ROBERT HOOKE - NAMED “CELLS” BECAUSE THEY LOOKED.

Zoology I Cytology, Embryology & Histology By Dr/ Alyaa Ragae Zoology Lecture Faculty of Oral and Dental Medicine Future University.

Simple to Complex – Life’s Levels of Organization

Forensic Instrumentation

MICROSCOPES F STEREO F COMPOUND LIGHT F TRANSMISSION ELECTRON (TEM) F SCANNING ELECTRON (SEM)

Measurement & Microscopes

Tools of a Biologist.

Theory vs Law Law – a fact Theory – an explanation for the fact Example… – Law = If I throw a apple up into the air, it will fall to the ground. – Theory.

Microscopes. Compound Light Microscope – Use lenses to magnify the image of an object by focusing light – Cell structures as small as 1 millionth of a.

Introduction to the Cell

Cycling of Matter in Living Systems 1.3 Developments in Imaging Technology and Staining Techniques.

The Cell Theory And how it developed.

A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.

Microscopy 1. UNITS OF MEASUREMENT 1 m = 1000 mm (millimeters) 1 m = 1000 mm (millimeters) 1000 mm = 1 µm (microns) 1000 mm = 1 µm (microns) Bacteria.

Microscopes Biology 11. The History 4 Many people experimented with making microscopes 4 Was the microscope originally made by accident? (Most people.

By: Rob Page and Tara Trovarello

Chapter 3: Cell StructureSection 1: Looking at Cells 1 Cell Structure Chapter 3.

MICROSCOPES A. COMPOUND LIGHT MICROSCOPES. FEATURES Magnification 10X to 400X Formula for determining total magnification – objective lens X ocular.

MICROSCOPES Some Important Vocabulary MAGNIFICATION- increase of an object’s apparent size RESOLUTION- the power to show details clearly * Microscopes.

Microscopes The invention of the microscope in the 17 th century led to the discovery of the cell. Robert Hooke described cells using this light microscope.

Sec. 4- Tools and Procedures Metric system-decimal system of measurement whose units are based on certain physical standards –Scaled on multiples of ten.

Introduction to the Microscope. Types of Microscopes The models found in most schools, use compound lenses to magnify objects. The lenses bend or refract.

Light Microscopes Light Microscopes Compound Light Microscope Focuses with light and lenses. Can be used with living specimens Light weight and portable.

Salman Bin Abdulaziz University College of Pharmacy Pharmacognosy Department Salman Bin Abdulaziz University College of Pharmacy Pharmacognosy Department.

Microscope Technology

Microscopes Biology 11. The History 4 Many people experimented with making microscopes 4 Was the microscope originally made by accident? (Most people.

Aim #10: What are some other tools scientists use in the laboratory?

The Microscope and Forensic Identification. Magnification of Images A microscope is an optical instrument that uses a lens or a combination of lenses.

An instrument for magnifying very small objects

Microscopy Microbial Biotechnology. Microscope an instrument for viewing objects that are too small to be seen by the naked eyes. First true microscope.

B-1.2: Use appropriate laboratory apparatuses, technology, and techniques safely and accurately when conducting a scientific investigation.

Microscopy Honors Biology Orders of Magnitude What would it look like to go from 10 million light years from Earth to an atom in an oak tree.

Introduction to the Microscope

Comparison b/w light and electron microscopes LIGHT MICROSCOPE ELECTRON MICROSCOPE Magnification can be done upto 2000 times Resolving power is less.

Compound Light Microscope

Microscopes Moisey (2009), Optical Microscope [photograph]. Retrieved from

Microscopy Group 2 Cabatit, Mendoza, Ramos, Rodriguez, Tan.

Presentation on.  There are many methods for measuring of fiber structure. Such as:  The absorption of infrared radiation  Raman scattering of light.

THE MICROSCOPE. HANS & ZACHARIAS JANSSEN Made the first compound microscope in 1595.

Basic Microscopy SC.912.L Compare and contrast structure and function of various types of microscopes. Image: The Far Side by Gary LarsonFrom the.

Starter: Microscopes Which image is from the light microsope? How do you know?

The Microscope.

Microscopes Lesson 3 September 24th, 2010.

Topic 1: Introduction to Histology

Microscopes Section 4.2.

Microscope What is the most widely used tool in biology? Microscope

Cell Biology Practical TEM&SEM

The Microscope.

A C B D 1. Read the information about different sorts of microscope.

“Viewing Cells”.

Microscopes Can you see me?.

Basic Microscopy SC.912.L Compare and contrast structure and function of various types of microscopes. From the Virtual Microbiology Classroom.

Microscopes Chapter 7.

Notes: Microscopes Microscope

Basic Microscopy SC.912.L Compare and contrast structure and function of various types of microscopes. From the Virtual Microbiology Classroom.

Presentation transcript:

Microscopes Light microscope Depth of field: small

Magnification Uses lenses arranged in series, made of optical material that bends light. All magnification takes place via lenses. Uses diascopic illumination (that is, light is transmitted through the specimen). Magnification up to about 1000x.

Light Microscope Uses: Pathology, microbiology, forensics, education Advantages: Color viewing. Require no electricity except for the light source. Can be used on living samples. Simple models are relatively common and inexpensive. Disadvantages: Usually requires prepared slides and cannot examine objects well in 3 dimensions.

Stereo or Dissecting Microscope Magnification: As in a slide microscope, but arranged in 2 optical paths at slightly different angles to allow the user to view objects in 3 dimensions. Uses episcopic illumination(light is reflected from the specimen’s surface). Maximum useful magnification is about 100x.

Stereo or Dissecting Microscope Depth of field: Large Uses: Microsurgery, fine repair, dissection, paleontology, quality control, sorting, forensics. Advantages: Color viewing. Can be used on living samples. Requires little or no preparation of the sample. Disadvantages: Low magnification

3D Digital Microscope Magnification: Works like a regular digital microscope to achieve up to 1000x magnification. Depth of field: Large, up to 20 times greater than that of a slide microscope.

3D Digital Microscope Advantages: Object can be viewed from almost any angle and its 3- dimensional features can be examined. Disadvantages: As with regular digital microscopes, can be expensive.

Transmission Electron Microscope Magnification : A series of electrostatic and electromagnetic lenses act on an electron beam to produce up to 50 million times magnification

Transmission Electron Microscope Depth of field: Very small Uses: Microbiology, pathology, crystallography Advantages: Very high resolution Disadvantages: Requires extensive specimen preparation, including staining & sectioning with an ultramicrotome. Cannot observe the surface of objects. Very specialized equipment that requires a partial vacuum.

Scanning Electron Microscope Magnification: Electrostatic & electromagnetic lenses, as with TEM. Magnification ranges from 25x to 250,000x. Depth of field: Large compared to that of TEM Uses: Biology, microbiology, geology, nanotechnology, crystallography Advantages: Can view objects’ 3- dimensional surface. Disadvantages: As with TEM, requires specialized equipment and a partial vacuum. Cannot be used on living specimens.