Laboratory Safety Training Department of Immunology 2014

Declaratory statements and concepts about lab safety according to the year 1993. XCIII. act 87. § regulation Workplace: every place or faciltiy outdoors/indoors, where employees are working or residing in order to perform labour. Organized labour: labour initiated, controlled or approved by the employer under normal employment, civil services or civil service legal relation in schools under studentship relation, during the technical training (or qualification) and corresponding to the professional requirements.

Physiological effects of electricity Accidents may occur if a human body joins in an electric circuit and an outer electric source gets through it electric current. Though the destructive effects of electricity originate in several physical and physiological influences, even so the accidents can be classified into two groups: Electric shocks and injuries which may come off jointly. 1. Electric shock occurs when an electric current flowing through the body and causes muscular spasm, disorder or paralysis of ganglia. 2. Electric injuries: a typical lesion at the very places of the entrance and exit of the electric current. Another characteristic impairment is the metallization of the skin: it appears at the contacting surfaces of the metal conductor and the skin, if the metal ions gets into the skin during the electrolysis.

Chemical safety The harmful effects of a chemical substance are depending on the circumstances and concentration in which the employee come into contact with. Alternatives of absorption: - surface of the skin - digestive organ - respiratory organ - conjunctiva of the eyes - ears The extraneous substance - after getting into the body – passes with the circulation and hinders the normal function of the organs. A single large dose exposure may cause acute a small but regular dose exposure chronic intoxication that may lead to occupational disease.

Fire safety regulations storage and usage of flammable liquids and substances usage of fire extinguishers fire safety regulations of working with an open flame (e.g. bunsen burner) possibilities and ways of fire alarm and appropriate behaviour due to a fire alarm

General lab safety regulations reporting obligation of accidents and injuries knowledge of ergonomic, workplace lighting instructions hygiene handling of machines and equipments use of safety clothing, tools and accessories use of aerator and vent hood regulations of handling and collection of hazardous waste use of hazardous substances and products smoking at workplace

Lab instructions during practice food and beverages are prohibited in the lab electronic eqipments must be turn off after experiments to flush out chemicals to the duct or drain is prohibited do not place flammable substances near to the radiator smoking is prohibited in the whole building

General instructions of first aid On the site of accident the first thing is a rapid orientation about: the number and status of injured people what happened a need for immediate intervention the order of attendance goal of first aid: to save life to inhibit subsequent health lesion to help healing

The role of aid donor to measure the site of accident quickly and correctly without risking his/her own safety to protect the site of accident without major alterations to attend the injured persons in order to seriousness (the most serious cases first)