LAB SAFETY Biology 112/111 http://www.nclark.net/Biology

General Safety Rules 1. READ & FOLLOW instructions carefully before attempting to do anything. 2. Wear GOGGLES to protect your eyes from chemicals, heated materials, or things that might be able to shatter. 3. TELL TEACHER if any spills or accidents occur.

General Safety Rules 4. After handling chemicals, always WASH YOUR HANDS with soap and water. 5. During lab work, keep your HANDS away from your face. 6. Tie back LONG hair.

General Safety Rules 7. Roll up LONG sleeves. 8. Know the LOCATION of the fire extinguisher, fire blanket, eyewash station, and first aid kit. 9. Keep your work area UNCLUTTERED. Take to the lab station only what is necessary.

General Safety Rules 10. It is suggested that you wear GLASSES rather than contact lenses. 11. Never put anything into your MOUTH during a lab experiment. 12. CLEAN your lab area at the conclusion of the laboratory period. 13. NEVER “horse around” or play practical jokes in the laboratory.

Glassware Safety 1. Chipped or cracked GLASSWARE should not be used. Show it to the teacher. 2. BROKEN glassware should not be disposed of in a classroom trashcan. There is a special glass disposal container for it under the sink. Use Broom & DUSTPAN

Chemical Safety 1. Wear protective GLOVES and a lab apron whenever heating or pouring hazardous chemicals. 2. Never MIX two chemicals together unless the teacher the teacher directs you to.

Chemical Safety 3. If you need to smell the odor of a chemical, WAFT the fumes toward your nose with one hand. Do not put your nose over the container and inhale the fumes.

Chemical Safety 4. Follow the INSTRUCTIONS of your teacher when disposing of all chemicals. 5. WASH your hands after handling hazardous chemicals.

Electrical Safety 1. Lay POWER cords where no one can trip on them or get caught in them. 2. Be sure your hands and your lab area are dry before using electrical equipment. 3. Never poke anything into electrical SOCKETS.

Electrical Safety 4. Unplug cords by pulling the PLUG and not the cord. 5. Unplug all electrical equipment at the END of the lab period.

Heating Safety 1. Let burners and hotplates cool down before touching them. Perform the SIZZLE TEST to see if they are cool enough. 2. Use tongs and/or protective GLOVES to handle hot objects. 3. Never reach across an OPEN flame or burner.

First Aid Injury: Burns What To Do: Immediately flush with COLD water until burning sensation is lessened.

First Aid Injury: Cuts, bruises What To Do: Do not touch an open wound without safety gloves. Pressing directly on minor cuts will STOP bleeding in a few minutes. Apply cold compress to bruises to reduce swelling.

First Aid Injury: Fainting To Do: Provide FRESH air and have the person recline so that their head is LOWER than the rest of their body.

First Aid Injury: Eyes What To Do: Flush eyes immediately with plenty of water (15 MINUTES at the eyewash). If a foreign object is lodged in the eye, do not allow the eye to be rubbed.

First Aid Injury: Poisoning What To Do: Find out what substance was responsible for the poisoning and alert the TEACHER immediately.

FIRE SAFETY http://www.youtube.com/watch?v=BLjoWjCrDqg

FIRE SAFETY Fires can either be extinguished by smothering the flames with a fire blanket or clothing OR a fire extinguisher! To use a fire extinguisher properly REMEMBER: P-A-S-S! PULL the PIN AIM at the BASE SQUEEZE the Trigger SWEEP side to side

Fires of organic matter that will leave ashes Always use the right type of fire extinguisher for each specific type of fire. *LHHS extinguishers (ABC type) CLASS A Fires: Fires of organic matter that will leave ashes Example: WOOD, paper, tissues

CLASS B FIRES: Fires of flammable LIQUIDS Examples: Gasoline, solvents NOTE: Water is not a good extinguisher because it spreads the liquid around.

CLASS C FIRES: Fires of an ELECTRICAL nature EXAMPLES: Computers, photocopiers

WHMIS Workplace Hazardous Materials Information Systems The 3 components to the WHMIS system are: MSDS Worker Education Labels on all Products

MSDS Material SAFETY Data Sheet Stored in binders in lab areas All known chemicals 8 sections (name, ingredients, physical data, fire hazards, reactivity data, toxicological properties, preventive measures, first aid measures)

WHMIS Labels – Workplace Hazardous Materials Information System Compressed GAS ex. Helium, propane Flammable/Combustible ex: methane gas, acetone Oxidizing Material ex: Chromic Acid Poisonous Infectious (IMMEDIATE & Serious Toxic Effects) ex: cyanide Poisonous Infectious (Other Toxic Effects) ex: mercury, lead Biohazardous Infectious ex: NEEDLES Corrosive Materials ex: degreaser Dangerously Reactive ex: pure sodium

HHPS Symbols Hazardous Household Product Symbols Levels of Severity (Danger, Warning and Caution)

Precautions related to Lab: Work in a well lit, well ventilated area Keep work area clean Properly organize work area and keep uncluttered The smell of formaldehyde is difficult to remove from belongings Carry microscopes properly, supporting the base and arm Know where MSDS sheets are located Look at the MSDS sheet: Formaldehyde Note: some specimens may be vacuum packed

Precautions related to Dissections: When working with preserved specimens be in a well-ventilated area Wear vinyl gloves at all times To reduce the irritating effects of chemical preservatives, rinse specimen under running water to eliminate excess and dilute what remains Review procedure for cuts and punctures from dissecting tools When cleaning dissecting instruments, hold sharp edges away from you.

More Precautions Related to Dissections: Properly mount specimen in dissecting pan Never remove specimen or specimen parts from the lab Clearly label and store specimens in designated area Always ask the teacher re: proper disposal of specimens…special containers for sharps, animal tissue and preservative disposal

When working with bacteriologic specimens: If you have open wounds or cuts on your hands, cover them with a bandage or wear latex gloves. Disinfect lab bench tops before and after handling bacterial cultures. While handling bacteria, keep your hands away from your eyes, nose, and mouth. Wash your hands thoroughly after handling bacterial If a culture is spilled, cover the spill area with paper towel and flood the paper towels with disinfectant. Wait 20 minutes and then wipe up the spill with more paper towels and dispose of the paper towel in a empty bag.

Acute Effect: An adverse effect on any living organism which results in severe symptoms that develop rapidly; symptoms often subside after the exposure stops. Chronic Effect: An adverse effect on a human or animal in which symptoms recur frequently or develop slowly over a long period of time.