LSBR Single particle processing workflow in Bsoft Bernard Heymann.

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Presentation transcript:

LSBR Single particle processing workflow in Bsoft Bernard Heymann

SPA Processing Steps Picking Corrections/Modifications (CTF, mask) Alignment Reconstruction Quality assessment (Resolution)

Bsoft SPA Workflow

Parameter Organization

Parameter Handling Flat files: Text files with tagged parameters Images referenced through tags Multiple formats: STAR & XML Backwards compatible Types of Bsoft parameter files: 1.Micrograph/reconstruction parameter files 2.Modeling parameter files

Quality Assessment Reconstruction: Resolution/spatial frequency limit. Modification curve (sharpening, B-factor) Number of particles in reconstruction Defocus range, astigmatism range View distribution Coverage/Completeness (up to spatial frequency limit) Particle FOM distribution Resolution: FSC curve or similar Modeling Fitting statistics

Reconstruction assessment View distributionResolutionFOM distribution