Osteogenic differentiation and mineralization in fibre- reinforced tubular scaffolds: theoretical study and experimental evidences by Vincenzo Guarino, Francesco Urciuolo, Marco A. Alvarez-Perez, Benedetto Mele, Paolo A. Netti, and Luigi Ambrosio Interface Volume 9(74): September 7, 2012 ©2012 by The Royal Society

Two step preparation scheme of PCL/PLA tubes (adapted from Guarino et al. [21]): (a) filament winding technology to integrate a PLA long fibre to the porous PCL matrix to obtain the tubular structure (left); (b) phase inversion and salt leaching technique t... Vincenzo Guarino et al. J. R. Soc. Interface 2012;9: ©2012 by The Royal Society

Effect of polymer/solvent ratio on structural properties of PCL/PLA tubes. Vincenzo Guarino et al. J. R. Soc. Interface 2012;9: ©2012 by The Royal Society

Effect of fibre cycle on structural properties of PCL/PLA tubes. Vincenzo Guarino et al. J. R. Soc. Interface 2012;9: ©2012 by The Royal Society

Modelling based on computational fluid dynamics (CFD) simulation. Vincenzo Guarino et al. J. R. Soc. Interface 2012;9: ©2012 by The Royal Society

Distribution of the wall shear stresses obtained by imposing three different flow rates: (a) 0.005, (b) 0.05 and (c) 0.5 ml min–1. Vincenzo Guarino et al. J. R. Soc. Interface 2012;9: ©2012 by The Royal Society

Radial perfusion bioreactor system. Vincenzo Guarino et al. J. R. Soc. Interface 2012;9: ©2012 by The Royal Society

Gene expression by RT-PCR of relevant bone-related makers (ALP (black), OC (light grey), OPN (dark grey)) and quantitative estimation of signal. Vincenzo Guarino et al. J. R. Soc. Interface 2012;9: ©2012 by The Royal Society

Mineral phase detection: Alizarin Red staining of PCL/PLA scaffolds as a function of perfusion flow rate. Vincenzo Guarino et al. J. R. Soc. Interface 2012;9: ©2012 by The Royal Society