Tactile Teaching: Exploring the Molecular World with Physical Models of Proteins and Other Molecular Structures by Tim Herman and Margaret Franzen Milwaukee School of Engineering Center for BioMolecular Modeling K12 Science Partnership Lunch American Society of Cell Biology 46 th Annual Meeting

The MSOE Center for BioMolecular Modeling We make physical models of molecular structures, using rapid prototyping technology. Science Education Outreach Mission Professional Development Program for High School Science Teachers Undergraduate Curriculum Development

x,y,z coordinates Protein Data Bank RP-RasMol Z Corp 3D-Printer Ply/Stl file Protein Models….in 4 Easy Steps Any image that can be created in the computer environment of RasMol,… … can now be converted to a physical model by rapid prototyping.

A Protein Model Starts with its PDB File. From the Protein Data Bank at Total Atoms 239 amino acids

MOLECULAR CAD…...its all about triangles. A Heme Group Each triangle,--- 3 points and a vector

Physical Models are Thinking Tools are (almost) universally effective in capturing students interest allow you to see things that are not obvious in computer-generated images. stimulate questions. are synergistic with computer visualization tools Physical Models Powerful

Exploring the Models I Water Kits… Amino Acid Starter Kit Because: Life happens in water. Proteins fold in water. How does a protein know what shape to fold up into? The basic laws of chemistry that drive protein folding.

Physical Models and Computer Visualization --- are Synergistic Example: How stringently are the Chemical Principles that Govern Protein Folding applied to real proteins? --- A Jmol Tutorial ---

Science Education Outreach Programs NIH SEPA Program Summer Course for High School Teachers Two Student Enrichment Programs SMART Teams Science Olympiad Protein Folding event NSF-CCLI Curriculum Projects Proteins in Active Learning Modules PALM Workshop – June 2007

The PALM Project Proteins in Active Learning Modules An NSF-CCLI Project Making the Molecular World Real through the use of: 1. Physical Models 2. Computer Visualization Tools (Jmol) 3. Illustrations 4. Flash Animations 5. Graphical Representations of Data Multiple Representations of Molecular Structures.

Molecular Stories Compelling molecular stories begin with Physical Models… …and are elaborated with an artful combination of Computer Visualizations Illustrations Animations Graphical Representations of Data …and a good teacher!

Two Molecular Stories Molecular Gymnastics Evolution in Action The Influenza HA Protein Acetylcholinesterase How the influenza virus hemagglutinin protein undergoes a dramatic conformational change upon endocytosis, leading to fusion of the viral and cellular membranes. How an effort to control mosquito populations by spraying with an insecticide inevitably leads to the emergence of resistant mosquitoes.

The MSOE Model Lending Library Physical Model Collections --- in suitcases Borrow for two weeks, just like a book from Inter-Library Loan No Cost, --- except for return postage. In Two Formats: Classroom Sets Discussion Props / Lab Stations

Introduction to Protein Structure Serine Protease Collection Ribosome Collection Membrane Transport Collection Zinc Finger Collection DNA Collection Acetylcholinesterase ~New~ Immunology Collection ~New~ Discussion Props and Lab Stations

Amino Acid Starter Kit© Water Kit© Collection NaCl Lattices Alpha Helix/Beta Sheet Collection ~New~ BioInformatics Map of the β-Globin Gene© DNA Discovery Kit© Mini-Toober β-Globin Folding Kit© MHC Class I Collection ~New~ Classroom Sets

The PALM Project Proteins in Active Learning Modules A 3-Day Summer Workshop for Undergraduate Faculty June 6-8, 2007 Milwaukee School of Engineering Models / Jmols / Animations / Illustrations Assessment Instruments

3D Molecular Designs A Small Business, spun off of the CBM A Dual Mission: To develop and commercialize innovative instructional materials To create custom protein models for researchers. R&D Program Supported by SBIR awards RP-RasMol design software; Water Kit; DNA Discovery Kit; Paper Bioinformatics Maps; Beta-Globin Toober Folding Kit etc.

Rapid Prototyping Technology An Additive Manufacturing Technology A Layer-by-Layer Approach Five Technologies Sterolithography Laminated Object Manufacturing Selective laser Sintering Fused Deposition Modeling Color Z Corporation 3D Printing