Nucleus and Mitosis VIBS 443/602.

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Presentation transcript:

Nucleus and Mitosis VIBS 443/602

Structure: pancreatic acinar cells Identification: nucleus, nucleolus, heterochromatin Heterochromatin Nucleus Nucleolus

Pancreas 158

158 Pancreas

32409

32409 Nuclei with nucleoli

Structure: electron micrograph of hepatocyte (EM 5) Identification: nuclear envelope, inner and outer membrane, nuclear pores Nuclear envelope Heterochromatin Euchromatin Nuclear pore

EM 2b cytoplasm nucleus

Structure: electron micrograph of stomach (EM 15) Identification: honeycomb nucleolus called nucleonema Nucleus Nucleonema

Structure: freeze etched of small lymphocyte (EM 6) Identification: fracture plane at one of the nuclear pores jumped from the outer to the inner membrane of the nuclear envelope Inner nuclear membrane Outer nuclear membrane Nuclear pores

Mitosis anaphase 102

102 Mitosis - metaphase

102 Mitosis - anaphase

Three major events in mitosis ORIENTATION SEGREGATION ALIGNMENT

CHROMOSOME MOVEMENT Three contributions Astral microtubules 1 3 2

152 201 Structure: intestinal absorptive cells Identification: nucleus, nucleolus Nuclei Nucleoli 152

201 Intestine crypt mitotic activity. Brush border Interphase nucleus Anaphase Artifact

duodenum 152

duodenum 152

duodenum 152

duodenum 152

duodenum 152

148 Ileum

32409

32409 Cells in mitosis

EM 10f: Arteriolar wall; 30,000x. Apical caveolae Collagen Dense bodies Elastic fiber Endothelial cell Nuclear pore RER

EM 19c: Sertoli cell; 13,300x. Golgi Heterophagic vacuole Lipid droplet Microtubule Nuclear pores (cross-section) Sertoli cell Synaptoneural complex

Colchicine treatment – chromosomes do not separate and NE forms around each chromosome due to breakdown of MT. Multinucleated cell

Cytochalasin B treatment – chromosomes separate into two normal size nuclei, but the cytoplasm does not split in two Cytochalasin B treatment-breaks down microfilaments-nucleus activity but cells do not undergo cytokinesis Binucleated cell Nucleolus

EVENTS IN MITOSIS CYTOKINESIS

Nucleus and Mitosis