DMD #031831 A comparison of whole genome gene expression profiles of HepaRG cells to human liver tissues, primary human hepatocytes, and HepG2 cells Steven.

Slides:

Advertisements

Similar presentations

Linear Models for Microarray Data

Advertisements

SHI Meng. Abstract The genetic basis of gene expression variation has long been studied with the aim to understand the landscape of regulatory variants,

GRAPHS OF MEANS How is a Graph of Means Constructed? What are Error Bars? How Can a Graph Indicate Statistical Significance?

Genomic Profiles of Brain Tissue in Humans and Chimpanzees.

CDNA Microarrays MB206.

Figure SOM1. Functional roles of the genes affected in zmet2-m1 mutants. Although the genes localized on the intracellular membranes were slightly over-represented.

Ishida et al. Supplementary Figures 1-3 Page 1 Supplementary Fig. 1. Stepwise determination of genomic aberrations on chr-13 in medulloblastomas from Ptch1.

Cell lineXY11q WM WM793-P WM793-P Lu ABAB WM793:WM793-P2WM793:1205-Lu WM793 WM793:WM793-P1 Supplemental.

CGH Data BIOS Chromosome Re-arrangements.

Expression profiling & functional genomics Exercises.

Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. Images of part of the erythrocytes under a differential interference contrast (DIC)

SUPPLEMENTAL FIGURE S1. Growth in BHI after 12 days at 4°C of L

Supporting Figure S4 (A) (B) (C) LOC_Os03G04630 LOC_Os03G04640

Volume 15, Issue 1, Pages (July 2014)

Two Way ANOVAs Factorial Designs.

آشنايی با اصول و پايه های يک آزمايش

Elevated hepatic SULT1E1 activity in mouse models of cystic fibrosis alters the regulation of estrogen responsive proteins Li Li, Charles N. Falany

A B C Supplementary figure S7

Kenneth G. Geles, Wenyan Zhong, Siobhan K

Volume 137, Issue 4, Pages (October 2009)

Volume 142, Issue 5, Pages e3 (May 2012)

Volume 13, Issue 9, Pages (December 2015)

Nociceptor-Enriched Genes Required for Normal Thermal Nociception

Volume 5, Issue 1, Pages (July 2015)

Figure S2 Supplementary data a b CCA1 CCA2 CCA4 CCA3 CCA5 EGFR HER2

Volume 122, Issue 4, Pages (April 2002)

Integrated protein co‐expression and differential expression across the ALS‐FTD disease continuum Integrated protein co‐expression and differential expression.

Cyclin E1 Is Amplified and Overexpressed in Osteosarcoma

Supplemental Figure S4. Expression changes of phased and non-phased siRNAs among the three tissues examined The expression changes of phased and non-phased.

Jinny Liu, Elizabeth Walter, David Stenger, Dzung Thach

8 slides for 4 panels (A-D)

Volume 3, Issue 1, Pages (July 2016)

Jinny Liu, Elizabeth Walter, David Stenger, Dzung Thach

(A) Growth curves of strains at 30°C in SPP medium or SPP supplemented with 15 μM paclitaxel or 10 μM oryzalin. (A) Growth curves of strains at 30°C in.

Volume 12, Issue 6, Pages (December 2003)

Daniel F. Tardiff, Scott A. Lacadie, Michael Rosbash Molecular Cell

Taming Human Genetic Variability: Transcriptomic Meta-Analysis Guides the Experimental Design and Interpretation of iPSC-Based Disease Modeling Pierre-Luc.

Michal Levin, Tamar Hashimshony, Florian Wagner, Itai Yanai

Volume 4, Issue 5, Pages (May 2015)

Volume 10, Issue 5, Pages (May 2018)

Molecular Therapy - Nucleic Acids

Volume 130, Issue 1, Pages (July 2007)

Brain-wide network synchronization.

Differential gene expression in whole blood from SJIA patients and healthy controls. A. Data were normalized in Beadstudio using the "average" method and.

Volume 5, Issue 4, Pages (October 2015)

Volume 132, Issue 6, Pages (March 2008)

Global Hypertranscription in the Mouse Embryonic Germline

A Major Role for Capsule-Independent Phagocytosis-Inhibitory Mechanisms in Mammalian Infection by Cryptococcus neoformans Cheryl D. Chun, Jessica C.S.

Volume 12, Issue 9, Pages (April 2002)

Expression and Histone H4 Acetylation Levels of MAPK6 and WRKY53 in the HDT701 OX and RNAi Plants after M. oryzae Inoculation. Expression and Histone H4.

Volume 7, Issue 2, Pages (August 2010)

* * * * * * * Supplemental Figure 3. GO Cellular Component Annotation Analysis of SA-Regulated Gene-Networks. We analyzed the genes in the gene-networks.

Comparison of N. gonorrhoeae gene expression in infected men in vivo and isolates grown in vitro. Comparison of N. gonorrhoeae gene expression in infected.

Volume 26, Issue 7, Pages e4 (February 2019)

Molecular Therapy - Nucleic Acids

Volume 23, Issue 12, Pages (December 2015)

FGF21 and the Second Coming of PPARγ

Kirst King-Jones, Michael A. Horner, Geanette Lam, Carl S. Thummel

Fig. 4. Expression of miR-2 family mediates tissue growth of Hippo pathway.(A) Photomicrographs of adult wings of the indicated genotype. Expression of.

Chd5 deficiency leads to compromised expression of the repressive histone mark H3K27me3 and up-regulation of ribosomal protein genes. Chd5 deficiency leads.

(A) Western blot probing nuclear extract from wild-type (wt) and the newly generated ACF1 mutant (AcfC) embryos (0–16 h). (A) Western blot probing nuclear.

7 WT 6 irx1-6 ixr fold increased expression 3 2 n- 1 AT1G18710

Whole-chromosome view of gene density, inverse environmental variation, inverse total variation, and open chromatin signature. Whole-chromosome view of.

CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured SGBS cells during adipocyte differentiation. CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured.

Construction of a Rice Glycosyltransferase Phylogenomic Database and Identification of Rice-Diverged Glycosyltransferases Cao Pei-Jian , Bartley Laura.

Volume 12, Issue 4, Pages (April 2019)

Overexpression of IL-27 upregulates expression of multiple IR by T cells in vivo. Overexpression of IL-27 upregulates expression of multiple IR by T cells.

Loyola Marymount University

ICOS+ and activated CD4+ T cells are dominant, tumour tissue-specific T cell populations in both mismatch repair-deficient and repair-proficient colorectal.

Differential Expression of RNA-Seq Data

Presentation transcript:

DMD # A comparison of whole genome gene expression profiles of HepaRG cells to human liver tissues, primary human hepatocytes, and HepG2 cells Steven N. Hart*, Ye Li*, Kaori Nakamoto, Eva-anne Subileau, David Steen, and Xiao-bo Zhong Supplemental Figure 1S. A comparison of the expression values in Log 2 scale among HepG2 cells, differentiated HepaRG cells, and primary human hepatocytes for CYPs in panel A, ADH in panel B, ALDHs in panel C, Non-CYP phase I enzymes in panel D, GSTs in panel E, NATs in panel F, SULTs in panel G, UGTs in panel H, ABCB/ABCC/ABCG in panel I, and SLCOs in panel J. When multiple sets of probes related to a gene are presented on the arrays, the probe that is most variable for a given gene is selected to represent the gene. The bars show the average expression values in Log 2 scale and the error bars indicate standard deviation. **p<0.01 and ***p<0.001 are used to indicate a statistically significant difference compared to primary human hepatocytes in a t test. UGT1A a corresponds to multiple UGT1A family members, including UGT1A1, UGT1A3, UGT1A4, UGT1A8, UGT1A9, and UGT1A10.

Supplemental Figure S1. *** ** *** ** *** ** *** ** *** A. CYPs ** *** ** *** ** B. ADHs Gene expression (Log 2 ) HepG2Diff HepaRGPHH

Supplemental Figure S1. ** *** ** *** ** *** ** *** C. ALDHs *** ** *** ** D. Non-CYP Phase I Gene expression (Log 2 )

Supplemental Figure S1. *** ** *** ** *** ** *** ** E. GSTs *** ** *** ** *** ** F. NATs Gene expression (Log 2 )

Supplemental Figure S1. ** *** ** *** ** G. SULTs *** ** *** ** *** ** *** H. UGTs Gene expression (Log 2 ) *** ** *** ** *** ** *** a Gene expression (Log 2 )

Supplemental Figure S1. ** *** ** *** ** *** ** *** ** *** ** *** ** I. ABCB/ABCC/ABCG *** ** *** J. SLCOs Gene expression (Log 2 ) **