DO NOW DYE TEST ACRINIDE ORANGE Stains DNA & RNA OSMIUM TETROXIDE A lab technician needs to determine whether cells in a test tube are prokaryotic or eukaryotic. The technician has several dyes she could use to stain the cells. Four of the dyes are described in the table to the right. Which dye could the technician use to determine whether the cells are prokaryotic or eukaryotic? DYE TEST ACRINIDE ORANGE Stains DNA & RNA OSMIUM TETROXIDE Stains lipids EOSIN Stains cell cytoplasm NILE BLUE Stains cell nuclei

Answer: Nile Blue Only eukaryotic cells have a nucleus. All of the other tests (DNA & RNA, cytoplasm and lipids) are characteristics shared by pro- and eukaryotes.

Objective: 1) To compare Unicellular and Multicellular organisms Diversity of Cells Objective: 1) To compare Unicellular and Multicellular organisms

I. Organisms may be: A. Unicellular organisms 1. 1 cell big B. Multicellular organisms 1. More than 1 cell 2. Cells may be organized into tissues, organs and systems

II. Unicellular Organisms A. Bacteria, protists, some algae B. One cell is responsible for entire work of keeping the organism alive 1. Hunt or make food 2. Breathe 3. Get rid of waste 4. Defense 5. Movement

C. Examples: 2. Euglena 1. Paramecium 3. Amoeba

III. Unicellular organisms have specialized structures: A. Movement: 1. Flagella 2. Cilia 3. Pseudopods B. Contractile vacuoles – pump out water C. Method of eating – oral groove

IV. Multicellular Organisms A. In most, cells are specialized to do a specific job 1. Red Blood Cells carry oxygen 2. Muscle cells contract, allow for movement B. These cells work together/rely on each other for survival 1. If RBC don’t carry oxygen to muscles, muscles can’t move c. Division of labor

V. Colonial Organisms A. Unicellular organisms that live in groups B. Attached to each other but have few specialized structures C. Bridge between uni and multicellular