BIO-PROCESS LAB (B) 2016 KAREN LANCOUR National Committee Chair-Life Science Bio-Process Lab National Supervisor karenlancour@charter.net

Event Rules – 2016 DISCLAIMER This presentation was prepared using draft rules.  There may be some changes in the final copy of the rules.  The rules which will be in your Coaches Manual and Student Manuals will be the official rules.

Event Rules – 2015 BE SURE TO CHECK THE 2016 EVENT RULES FOR EVENT PARAMETERS AND TOPICS FOR EACH COMPETITION LEVEL

TRAINING MATERIALS Training Power Point – content overview Training Handout - content information and needed skille Practice Activities - sample stations with key Sample Tournaments – sample problems with key Event Supervisor Guide – prep tips, event needs, and scoring tips Internet Resource & Training CD’s – on the Science Olympiad website at www.soinc.org under Event Information Biology-Earth Science CD, Bio-Process Lab CD (updated 2015) in Science Olympiad Store at www.soinc.org Biology lab manuals – identify variables and evaluate the labs – become familiar with typical bio labs

Bio-Process Lab (B) Event Description - lab-oriented competition involving the fundamental science processes of a middle school biology lab program Event – lab practical in stations Event Parameters – be sure to check the rules for resources allowed, type of goggles needed.

Basic Science Process Skills Observing Measuring Inferring Classifying Predicting Communicating

Integrated Science Process Skills Formulating Hypothesis Identifying Variables Defining Variables Operationally Describing Relationships Between Variables Designing Investigations Experimenting Acquiring Data Analyzing Investigations and Their Data Understanding Cause and Effect Relationships Formulating Models

GAME PLAN USE THE POWERPOINT FOR OVERVIEW USE THE HANDOUT FOR DETAIL OF INFORMATION NEEDED AND TYPE OF QUESTIONS GO TO THE INTERNET RESOURCES AND CD’S FOR MORE HELP DO THE PRACTICE ACTIVITIES TO MASTER SKILLS DO THE SAMPLE TOURNAMENTS UNDER TIMED CONDITIONS TO EXPERIENCE COMPETITION SITUATION DO OLD TOURAMENTS– PRACTICE, PRACTICE, PRACTICE THE KEY TO SUCCESS – THE BEST WAY TO LEARN THE SKILLS IS BY DOING

Student Preparation Team work skills – work as a team Many mistakes are made when work is split to save time – results in many errors Time limits – invest in a timer and practice using it Check station setups – to see what is available to help you Answering questions – see student preparation guide Be sure to read questions and do all parts requested Measurement and Calculations – check graduations and remember units Reference materials – make them functional for competition under timed conditions Practice, Practice, Practice using the skills you have learned

Compound Microscope Parts Making wet mount Appearance of objects Movement of objects Magnification Changing objects Estimating size of objects Field diameter & area

Principles of Microscopy

Measuring Objects under Microscope

Sample Station – Microscopy Determine the diameter of the field and the length of one cell in mm and convert to mcm.

Stereomicroscope Parts Appearance of objects Magnification Advantages Uses Observing objects

Examining Instrument Graduations Before Measuring Capacity- highest it will read – starts at zero Range if does not start at zero as thermometer Numbered Increments (graduations) Unnumbered Increment (graduations) MANY ERRORS ARE MADE BECAUSE STUDENTS DO NOT EXAMINE THE GRADUATIONS BEFORE MEASURING !!!!!

Measuring Liquids Estimating Meniscus – read bottom Capacity and Range Graduations – numbered and unnumbered increments Readability Making measurements Estimating

Metric ruler and calipers Capacity and Range Numbered and unnumbered increments cm vs mm Uses of each Making measurements Estimating Vernier Scale

Thermometers Capacity and Range Do not start at zero Numbered and unnumbered increments Uses of each Making measurements Estimating

Sample Station - Measurement Identify range, capacity, incrementation Do measurement – estimate last digit

Triple Beam Balance with Weights Expands capacity of the balance from 610g to 2610 grams 2 weight equivalent to 1000 g and 1 weight equivalent to 500 g Actual mass of weight is listed on the top of the weight.

Triple Beam Balance Capacity – auxillary weights Units – numbered and unnumbered increments Tare Using the Balance Advantages & Disadvantages

Electronic Balance Capacity Units Tare or Zero Err Using the Balance Advantages & Disadvantages

Probes Graphing calculator Easy link or CBL Probe Collect data onto calculator Transfer data to computer Graph analysis Quick data collection

Sample Station – pH Determine the pH of various solutions using either pH probe or pH test papers.

Data Presentation and Analysis- Data Tables Format Title Units of measurement Numbering Tables Source Leg (thigh) Length (cm) Time of 40 yard dash (sec) 24 9 31 9.2 37 11 38 10 39 8.2 42 8.4 55 9.3 62

Data Presentation and Analysis-Graphs Graph – types X vs Y axis Scaling axis Plotting points Human Error Curve or best fit line Labeling

Sample Stations – Population Density Measure sample area Determine population density for symbols Assign an organism to symbols Form a food chain Evaluate sample – predict techniques, etc.

Experimental Analysis Identify variables Examine procedure – will it evaluate allow the hypothesis to be tested? Evaluate observations to understand what happened and why Analyze data and identify errors Determine if the hypothesis is true – if false what was not considered? Propose further testing or new hypothesis

Sample Station – Experiment Analysis Analyze the design of the experiment Identify variables Explain results – form conclusions

Data Analysis Use observations to understand what happened during the experiment Look for possible types errors Look for patterns in the data Do data analysis as mean, median, mode Examine group vs. class results data Use data to evaluate hypothesis

Sample Station – Data Analysis Making hypotheses Food web analysis Eating habit analysis Predictions and conclusions Inferences

Human Mistakes vs. Experimental Errors Human Mistakes – carelessness Experimental Error – instrument variation or technique Random Error – chance variation Systematic Error – system used for designing or conducting experiment

A Sample Dichotomous Key 1. wings covered by an exoskeleton . . . . . . go to step 2   1. wings not covered by an exoskeleton. . . . go to step 3 2 body has a round shape. . . . . . . . ladybug   2 body has an elongated shape. . . . . .grasshopper   3.wings point out from the side of the body . . dragonfly 3 wings point to the posterior of the body.. . . . Housefly Note: There should be one less step than the total number of organisms to be identified in your dichotomous key.  

Sample Station – Dichotomous Key Have specimens of leaves Formulate a key or use a key to identify specimens

Key to Success in Bio-Process Lab Learn the Skills – using Practice Activities Practice under Timed Conditions Work as a Team – saves time and catches errors Pay attention to details – avoid silly mistakes Relax and let the competition show you how much you have learned Have fun !!!