An Experimental Model of Human Absorptive Enterocytes (Caco-2) to Assess the Bioavailability of Various Metals Jonas Tallkvist Department of Biomedical.

Slides:

Advertisements

Similar presentations

Lead and Other Contaminants in Baltimore Urban Garden Soils: What You Can Do to Protect Your family Rufus L. Chaney USDA-Agricultural Research Service.

Advertisements

Need for and prevalence of separate guidelines List of substances: Ba, Cu, CN -, F -, Ni, Phenol, V ( cadmium evaluated, default SCTL protective of acute.

Chemical Equations Preparation for College Chemistry Columbia University Department of Chemistry.

 Single Replacement Reactions + + . General Equation A + BX  AX + B.

P-saturated Ochre: Performance as a Fertiliser and Environmental Acceptability K.E. Dobbie, K.V. Heal and K.A. Smith School of GeoSciences, University.

75 years of excellence 16 June 2011 – Washington DC 16 th Trilateral Working Group Meetings Agenda Item #7 Lithium Coin Cell Ingestions 16 th Trilateral.

Metals and alloys in external constructions – changes in speciation and bioavailability of corrosion-induced metal runoff upon environmental entry. Associate.

Effects of Anti-Epileptic Drugs (AED), Phenytoin and Carbamazepine, on Calcium Transport in Caco-2 Cells, an Immortalized Human Intestinal Cell Line Melinda.

LABORATORY METHODS for LEACHATE ANALYSIS RESULTS Temporal and cumulative metal leaching mass. Cumulative leaching (per ha) in control treatments were:

Introduction Cadmium (Cd) is a toxic transition metal with LC 50 of mg/m 3 for 4 hours of exposure. It forms 2+ ions. Copper(Cu) is a nutritive transition.

Lecture 162/24/06. Quiz 1. A 0.02 M solution of an acid (HA) produces a pH of 5. What is the K a of the acid? 2. If you mix equal amounts of HBr and Na(CH.

Lecture 142/21/07. Metal hydrolysis Heavy metals as Lewis acids Fe(H 2 O) 6 3+ (aq) ⇆ Fe(H 2 O) 5 (OH) 2+ (aq) + H + (aq) Fe(H 2 O) 6 2+ (aq) ⇆ Fe(H.

Lecture 223/19/07. Displacement reactions Some metals react with acids to produce salts and H 2 gas Balance the following displacement reaction: Zn (s)

Comparison of Soils and Leaves of Pacific Madrone and Their Relationship to Plant Pathology A.B. Adams and Rand Knight.

The Effect of Non-Composted and Composted Soil on Nutrient Concentrations in Green Beans By Nicol, Scott, and Jenn.

29 th International conference SEGH, 8-12 July Toulouse, FRANCE 2013 Health risk estimate for groundwater and soil contamination in the.

The Schrödinger Model and the Periodic Table. Elementnℓms H He Li Be B C N O F Ne.

Zinc Smelting What role did it play in the Donora disaster?

MINERALS REQUIRED IN LIVESTOCK DIETS Major minerals (Required in large quantities) Beef Dairy Swine Chicken Finishing Cow Dry Lactating Finishing Sow Broiler.

Significance Caffeine and ibuprofen may negatively affect plant growth.  Caffeine and ibuprofen generally exist at concentrations below 1 ppm in surface.

S. Sauvé1,2, A. Dumestre1, M. McBride1 and W. Hendershot2

Activity series Experiment # 15.

Mobility and bioaccessibility of inorganic contaminants in soils in the vicinity of copper smelters (Copperbelt, Zambia) Vojtěch ETTLER 1, Martin MIHALJEVIČ.

1 DETECTION OF COPPER IN THE SYNTHETIC WASTEWATER BY USING PYRAZOLIDINE LUMINOL (PL) Syarifah Hikmah Julinda a, Preeda Parkpian a and Srung Smanmoo b a.

Bioavailability Dr Mohammad Issa.

Determination of bioavailable macro- and microelements from agricultural soil using different extractants 1.Introduction Translocation of elements from.

ICP MAKING STANDARDS. NUMBER OF STANDARDS Determine the number of standards necessary for your measurements (Blank, 100ppb, 500ppb, 1000ppb, etc)

Activity Series A method for predicting reactions.

Reactions Reference. Solubility Rules 1.All nitrates, acetates, and chlorates are soluble. 2.All chlorides, bromides, and iodides are soluble except for.

Sediments Toxicity of the rivers draining Ibadan Metropolis Southwestern, Nigeria. 1 Ajibade, O.M and 2 Abimbola, A.F. 1 Department of Earth Sciences,

MAJOR CHEMICAL COMPONENTS OF THE LIVING ORGANISMS Medical Biochemistry Molecular Principles of Structural Organization of Cells.

Iron and Copper Transport via DMT-1 in HEK-293 Cells Alex Jackson, York College of Pennsylvania Biology Department Introduction Neurodegenerative diseases.

13.2a Developing a Redox table.  the relative reactivity of metals can be used to determine which redox reactions are spontaneous In all redox reactions,

CHARACTERIZATION OF NATURAL AND ENGINEERED NANOPARTICLES: SHAPE, SIZE AND CHEMICAL COMPOSITION Lucia Manangon

Predicting Products Chemistry General Chemistry Ch 11 Accelerated Chemistry Ch 3.

Reactions of Metals. Reactions of Metals with H 2 O The metal is the anode and will be oxidized. 2H 2 O + 2e-  2OH - + H 2 E° = V Mg  Mg 2+ +

The Activity Series How Do We Know If a Reaction Will Occur?!? The Activity Series tells us what will and what will not react ACTIVITY SERIES: a ranking.

Redox Reactions Oxidation - Reduction reactions Terms Oxidation loss of electrons electrons are a product Na --> Na + + e - Reduction gain of electrons.

Single Replacement Reactions  A single replacement reaction occurs when atoms of an element replace the atoms of a second element in a compound.  This.

Simultaneous sensing and scavenging of heavy metal ions in water using cellulose nanocrystal - nanocluster composites Nishil Mohammed 1, Avijit Baidya.

Topic: Families of Elements PSSA: A/S8.C.1.1.

Bell Ringer Complete and balance each of the following synthesis reactions by writing chemical equations. a. Na + O2→ ______ b. Mg + F2 → ______ a. 4Na.

Results Objective Assess the effects of metal interactions on trace metal uptake by plant Evaluate the effects of plant uptake process on the distribution.

Date of download: 10/22/2017 Copyright © ASME. All rights reserved.

Single Replacement Reactions

Predicting Reactions.

Reactions of Metals.

Figure 1 Metal bioimaging in 30-μm thick cryosections taken from the brains of untreated and AAV8-AAT-co-miATP7B-treated Atp7b null mouse Metal bioimaging.

كيمياء العناصر الانتقالية

Formation of a solid AgCl

About Plasma and intestinal concentrations of sulfadiazine-trimethoprim in pigs after (non)conventional oral and intramuscular treatment, within the context.

نانو سرامیک‌های مورد استفاده در دارو‌رسانی

طيف سنجي نشر شعله اي ،جذب اتمي و فلوئورسانس اتمي

Speed Dating Speed Dating H Na Speed Dating Speed Dating K Be.

Soil processes and trace metals

Cations (mg/l) Wavelength Trace Elements (µg/l) Wavelength

המרכז הפדגוגי לעובדי הוראה, ירושלים, אילנה זהר ©

Pharmacokinetics: Drug Absorption

سامانه مدیریت آزمایشگاه‌های دانشگاه یزد

DETECTION LIMITS < 1 ppt ng/L 1-10 ppt ng/L ppt ng/L

© The Author(s) Published by Science and Education Publishing.

Pharmacokinetics: Drug Absorption

Predicting the Product in Single Replacement Reactions

First Approach to the EMEP Assessment Report – Slovak Republic

Increase in Ease of Oxidation

Group 1 Group 2 Group 3 Group 5 Group 4 Ag+, Pb2+, Hg22+

Table 6. Bioaccumulation factors for heavy metals in rice crop

© The Author(s) Published by Science and Education Publishing.

Model for transporter activity for mammalian manganese absorption, hepatic uptake, and endogenous excretion. Model for transporter activity for mammalian.

Table 1. Chemical Composition of Base Aluminium Alloys

Presentation transcript:

An Experimental Model of Human Absorptive Enterocytes (Caco-2) to Assess the Bioavailability of Various Metals Jonas Tallkvist Department of Biomedical Sciences and Veterinary Public Health, Division of Pathology, Pharmacology and Toxicology, Swedish University of Agricultural Sciences, Uppsala, Sweden MITF

Bioavailability ”The fraction of ingested metal that reaches the systemic circulation”

Factors Influencing Bioavailability Dose Age pH Gastrointestinal contents Nutritional status Disease

Metals Investigated in Caco-2 Cells V Co Fe Mn Ca Cu Cd Zn Ni Pb Cr Mg

Routes of Metal Absorption Basement membrane Blood vessel Mucosal/apical side Blood/basolateral side Enterocytes ActivePassive

The Caco-2 Cell Model Apical chamber Basolateral chamberPermeable filter support Caco-2 cells

Cadmium Absorption Zalups and Ahmad (2003) Toxcol Appl Pharm, 186, 3

Tallkvist et al., (2001) Tox Lett, 122, 171 Correlation Between DMT1 Expression and Cd Transport in Caco-2 Cells

Summary The Caco-2 cell model provides a useful tool to assess the bioavailability of metals at various defined experimental conditions. The Caco-2 cell model offers the possibility to gain insight into molecular mechanisms implicated in the bioavailability of metals.

Thank-You for Your Attention!