Scrolling clicker attendance See if you can figure out how to “click-in” on the scrolling screen to alert the computer that you are here.

Announcements Course Pack glitch: You missing stuff? New textbook: opportunity is causing change Review contract: If you accept this plan, and your responsibilities, submit a signed copy. i>clickers: now start to gain A&P points Readings and Homework on Angel or D2L? Stand up if completed reading (Watson+ )

Career Goals of class Doctor for humans: 22 students Doctor for animals: 3 students Lab- Medical, Forsensic, DNA Research: 3 Software: 1 student Marine Biology: 1 student Undecided: 4 students

Thinking of what you want to get out of your college education and this course, which of the following is most important to you? a)Acquiring information (facts, principles, concepts) b)Learning how to use information and knowledge in new situations c)Developing lifelong learning skills

Of these three goals, which one do you think you can make headway on outside of class by your own reading and studying? a)Acquiring information (facts, principles, concepts) b)Learning how to use information and knowledge in new situations c)Developing lifelong learning skills

Work outside of class by your own reading and studying a)Acquiring information (facts, principles, concepts) b)Learning how to use information and knowledge in new situations c)Developing lifelong learning skills

Work inside of class together so we can make headway on these. a)Acquiring information (facts, principles, concepts) b)Learning how to use information and knowledge in new situations c)Developing lifelong learning skills

Outside class/Prior to lecture (2hrs/day) Read, take notes (Highlight), do homework. Review- Study notes prior to start of lecture Inside class/During lecture Review & solve problems- focus part of topic Annotate - your notes, add new thoughts (Bring questions- ask Qs you encountered) High (university-level) expectations

Extreme opportunities Time to learn: Not 1 chapter per lecture Lab Course: 1 lab experiment, 1 paper, 4 people Exams – take-home open book, verbal final (opt) Clickers – approx ½ participation, ½ extra credit Resume gets fat & confidence high, after lab work. Course average grade ~85%

What is a 4.0? In the university grading system PERCENTAGE of mastery = PERCENTAGE grade Quantity

What is a 4.0? In the university grading system Quality Quality of work = Level of grade

What is a 4.0? In the university grading system If a student does what was asked of them on an assignment and does a pretty good job what’s the grade supposed to be? a.4.0 b.3.5 c.3.0 d.2.5 e.other

What is a 4.0? In the university grading system The “4.0” is Outstanding is the student blew you away with how much and how well they did the work. They taught you something you didn’t know. A “3.5” is Most Excellent every detail of their work was done extremely well and they found additional papers and evidence beyond what they were told/expected to do, to complete their work. A “3.0” is Excellent is impressive work, top of the class, and their work was done extremely well but nothing beyond what was expected "Pretty Good" the student did the work required and a pretty good job, the average in the class.

Reading for today began with [ELSI, GATTACA, Eugenics] What is ELSI? Why did the authors say making/taking a diagnostic DNA test for Huntington’s disease is controversial? GATTACA? Eugenics?

Integrating Questions 1. Which carbon in dATP is missing the oxygen found in ATP? Which deoxyribose carbon is attached to the string of three phosphates? A. Carbons # 3’ and 5’ respectively B. Carbons # 2’ and 3’ respectively C. Carbons # 2’ and 5’ respectively D. Carbons #2 and 3 respectively E. None of the above

Integrating Questions 2. Speculate why a triple- stranded DNA molecule with the negatively charged phosphates in the center would be unstable and thus not a good model. A. phosphates would attract B. phosphates would repel C. phosphates are too big to fit D. phosphates would form covalent bonds E. None of the above

Integrating Questions A. One strand’s words should spell backwards B. One strand’s phosphates should move inside C. One strand should be upside-down D. More than one hydrogen bond should be there E. None of the above 3. Look at the Figure and find the misleading aspect of the diagram, given the two strands are supposed to be anti-parallel. What should be done to correct this historic figure?

Integrating Questions A. A=T have two hydrogen bonds B. G=C have three hydrogen bonds C. G + A are bigger and C + T are smaller D. two bases cannot pair with just one E. None of the above 4. If the DNA had a uniform diameter of 20 Å, why did the bases have to pair one A for each T, and one G for each C base?

Integrating Questions 1. (write) What’s the controversy connected to these famous images? [Who, what, when, why?]

Integrating Questions 2. What’s interesting about this famous image? [Who, what, where, when, why?]

Pre-test: Experimental Design

How many CFTR channels are on the surface of a cell of a CF patient?? [Normal=1000] A B. 250 C. 100 D. 50 E. None of the above are correct.

Where are the CFTR channels found normally [in a healthy person]? A. the apical surface -> B. inside the ER C. the lateral surface -> D. in the lysosome E. the basal surface ->