General Laboratory Tools Techniques and Methods Browse through these files to get familiar with equipment, techniques and general approaches to use in.

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General Laboratory Tools Techniques and Methods Browse through these files to get familiar with equipment, techniques and general approaches to use in your laboratory work this semester to use in your laboratory work this semester. This is your ‘toolbox’

‘Toolbox’ content ‘Toolbox’ content General techniques- equipment use Glassware glossary Volume measurement Balances Error analysis Excel for lab work Heating Melting Point pH meters Waste disposal HOW TO: Determine concentration of unknown in solution Prepare solutions Mixture separations Identify molecular or ionic species Identify ions in solution How to measure heat exchange Measure how fast a chemical reaction proceeds (rates of reactions) Make buffers and how to use them

General Techniques-Equipment Use

Excel use for lab work Excel use for lab work

Heating Equipment

pH meters pH meters

Volumetric glassware

HOW TO Determine the concentration of unknown species in a solution?

A. Titration-based on aqueous reactions Acid-base titrations Complexometric titrations ◦Back titration calculation

B. Spectroscopy-based on light absorption properties UV-Vis IR MS NMR

HOW TO prepare solutions?

B. Dilution of available solutions Going form a high concentration solution to a low concentration solution Serial dilution Parallel dilution

HOW TO: separate the components in a mixture?

Types of Separations Solid-Solid Solid-Liquid Liquid-Liquid

HOW TO: determine whether a solution is ionic or molecular?

A. Solubility IN polar and non-polar solvents

B. Melting/Boiling Point

C. Conductivity Does it conduct electricity? ◦Conductivity meter

HOW TO : identify ions in solution?

A. Aqueous chemical reactions Based on solubility properties

B. Flame test What’s the emission spectra?

C. Spectroscopy Based on energy absorption properties UV-Vis IR MS NMR

How do you determine the rate of chemical reactions? HOW TO measure the ‘activity’ of a catalyst?

A. Example: progression of a reaction catalyzed by an enzyme ◦Spectroscopy ◦Reaction rates ◦pH

HOW TO make and why to use Buffer solutions? Why do you need buffers? How do you prepare a buffer solution? How do you select a buffer? How does a buffer work?

HOW TO measure the heat exchange in a chemical reaction?

Calorimetry Constant-pressure calorimetry Constant-volume calorimetry