Prodigiosin Synthesis

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Prodigiosin Synthesis UV-induced “Colorless” Mutants of Serratia marcescens

The Normal Pathway to Prodigiosin b(g) enzyme x amino acids Prodigiosin enzyme z c d(ws) O N H enzyme y

Serratia marcescens D1 (wild type) (DNA is not mutated for any of the genes) A b(g) enzyme x amino acids Prodigiosin enzyme z C d(ws) enzyme y

Serratia marcescens D1 (wild type) (DNA is not mutated for any of the genes) B(g) a enzyme x amino acids Prodigiosin enzyme z D(ws) c enzyme y

Serratia marcescens D1 (wild type) (DNA is not mutated for any of the genes) a b(g) enzyme x amino acids Prodigiosin enzyme z c d(ws) enzyme y

Serratia marcescens ( ) (DNA is mutated in enzyme x gene) a b(g) enzyme x amino acids Prodigiosin enzyme z c d(ws) enzyme y

Serratia marcescens ( ) (DNA is mutated in enzyme x gene) A b(g) enzyme x amino acids Prodigiosin enzyme z C d(ws) enzyme y

Serratia marcescens ( ) (DNA is mutated in enzyme x gene) A b(g) enzyme x amino acids Prodigiosin enzyme z D(ws) c enzyme y

Serratia marcescens ( ) Serratia marcescens ( ) (DNA is mutated in enzyme x gene) A b(g) enzyme x amino acids Prodigiosin enzyme z D(ws) c enzyme y a b(g) enzyme x amino acids Prodigiosin enzyme z c d(ws) enzyme y Serratia marcescens ( ) (DNA is mutated in enzyme y gene)

Serratia marcescens ( ) Serratia marcescens ( ) (DNA is mutated in enzyme x gene) A b(g) enzyme x amino acids Prodigiosin enzyme z D(ws) c enzyme y A b(g) enzyme x amino acids Prodigiosin enzyme z C d(ws) enzyme y Serratia marcescens ( ) (DNA is mutated in enzyme y gene)

Serratia marcescens ( ) Serratia marcescens ( ) (DNA is mutated in enzyme x gene) A b(g) enzyme x amino acids Prodigiosin enzyme z D(ws) c enzyme y B(g) a enzyme x amino acids Prodigiosin enzyme z C d(ws) enzyme y Serratia marcescens ( ) (DNA is mutated in enzyme y gene)

Serratia marcescens ( ) Serratia marcescens ( ) (DNA is mutated in enzyme x gene) A b(g) enzyme x amino acids Prodigiosin enzyme z D(ws) c enzyme y 933 WCF B(g) a enzyme x amino acids Prodigiosin enzyme z C d(ws) enzyme y Serratia marcescens ( ) (DNA is mutated in enzyme y gene)

Serratia marcescens ( ) Serratia marcescens ( ) (DNA is mutated in enzyme x gene) 933 B(g) A b(g) enzyme x amino acids Prodigiosin Prodigiosin enzyme z D(ws) c enzyme y 933 WCF B(g) B(g) a enzyme x amino acids Prodigiosin enzyme z C d(ws) enzyme y Serratia marcescens ( ) (DNA is mutated in enzyme y gene) WCF

Serratia marcescens ( ) Serratia marcescens ( ) (DNA is mutated in enzyme x gene) 933 A b(g) enzyme x amino acids Prodigiosin enzyme z D(ws) c enzyme y 933 WCF D(ws) B(g) a enzyme x amino acids Prodigiosin Prodigiosin enzyme z C D(ws) d(ws) enzyme y Serratia marcescens ( ) (DNA is mutated in enzyme y gene) WCF

Serratia marcescens D1 (wild type) (DNA is not mutated for any of the genes) a b(g) enzyme x amino acids Prodigiosin enzyme z c d(ws) enzyme y 933 WCF Why is D1 (so capable!) such a poor donor to either mutant… D1 933 D1 WCF b(g) d(ws) 933 WCF D(ws) B(g) …compared to cross-feeding from either mutant?