The nucleus accumbens is a brain region involved in motivation and reward to natural stimuli such as food. This function is regulated by for the neurotransmitter dopamine. The nucleus accumbens is also implicated in addiction to drugs such as cocaine and amphetamines which act by increasing the level of extracellular dopamine. Understanding the role of the nucleus accumbens and its behavioral effects has many clinical applications for understanding eating disorders, drug addiction and Attention Deficit Hyperactivity Disorders (ADHD).The nucleus accumbens has two major subdivisions: the shell and the core. This study focuses on the nucleus accumbens shell (AcbSh). Previous studies have demonstrated that the AcbSh controls motivation for food. Salt is a strong natural motivator and plays an important physiological role in the body. The goal of this study is to further explore the role of the AcbSh in the motivation for salt intake. Effect of Nucleus Accumbens Shell Inhibition on Salt Intake Frehiwot Gebrehiwot, RET Fellow 2010 Science Teacher, Morgan Park High School RET Mentor: Dr. David Wirtshafter NSF-RET Program Discussion Materials and Method Results  National Science Foundation Grant EEC  Professor Andreas Linninger, Program Director  Seon B. Kim, Program Assistant  Dr. David Wirtshafter, Research Mentor  University of Illinois at Chicago  Co-Investigators: Beth Cowgill and William Schulenberg Cannulae Implant and Lickometer.  Animals: a total of 24 rats were used  Surgery: Stainless steel guide cannulae were implanted in the scalp aimed at the AcbSh and were held in place by stainless steel screws and dental cement  To induce salt appetite, rats received a subcutaneous injection of furosemide, a diuretic with the dose adjusted for body weight  To confirm diuresis, pre and post injection body weighs were compared  Using guide cannulae, rats received microinjection of the inhibitory drug muscimol, a GABA agonist, Into the AcbSh. The control group received a microinjection of saline solution  Rats were placed in lickometers with pre-measured quantity of 3% salt solution for 60 minutes  Several behavioral parameters such as total number of licks, latency to licks, numbers of bursts, number of clusters were recorded using a computer system Figure 1: Mean of number of licks recorded during 60 minutes testing. Difference between the two groups is t= (P= 0.984) Statistical Analysis: The t-test value for the number of licks is t= and P= The difference in the mean values between the two groups is licks which is not great enough to reject the possibility that the difference is due to random sampling variability. There is not a statistically significant difference between the experimental and the control group. The average number of licks per 5 minutes period also shows no difference in the pattern of drinking between the two groups. Conclusion: Other studies have shown that inhibition of the AcbSh by muscimol produces large increases in the consumption of sucrose and corn oil, but decreases intake of saccharine and ethanol. The results of the present study indicate that inhibition of the AcbSh by muscimol does not have an effect on salt intake behavior. The next stage in the study is to conduct a histological examination of the brain of tested rats to check for correct placement of cannulae into the AcbSh. Figure 2: Data shows that there is no significant difference in pattern of consumption of salt solution during testing. Acknowledgements Consumption of 3% Salt Solution After Injections into the Accumbens Shell Average Number of Licks per 5 minute Bin After Injections into the Accumbens Shell Introduction Brain: Nucleus Accumbens Visible in Red