ProteoRed WG1-WG2 Meeting Pamplona, December 10th 2013 PME10 PROPOSALS.

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ProteoRed WG1-WG2 Meeting Pamplona, December 10th 2013 PME10 PROPOSALS

MSRT1 SIGMA-ALDRICH

Objectives: 1) Test Rt standards for SRM methods inter-platform interchangeability 2) Multilaboratory test targeted quantification of Chr16 proteins Experiment Design: - Sample: total lysate extract of a model cell line in SpHPP-Chr16 ? - SRM SpHPP validated methods - Rt stds- Internal / External - Different gradient lenghts min - Labeled peptide standards for selected proteins -> absolute quantitation PME10