Сombination of Nanoparticles and Photosensitizers in Photodynamic Action on Microorganisms Pavel O. Petrov, Ivan A. Orlov, Alexandr V. Kovalev, Elena S.

Slides:



Advertisements
Similar presentations
EMB agar Only gram-negative bacteria grow on EMB agar. (Gram-positive bacteria are inhibited by the dyes eosin and methylene blue added to the agar.) Based.
Advertisements

LINEZOLID LINEZOLID The discovery and clinical development of effective antibiotics is most remarkable achievement over the past 60 years.. Since the.
EX. 20 Antibiotic Disk Sensitivity
CULTURAL AND MORPHOLOGICAL PROPERTIES OF THE VACCINE STRAIN OF BACTERIA Y. PESTIS EV NIIEG AFTER PHOTODYNAMIC INACTIVATION Onega Ulianova, Saratov State.
Infection control Antiseptics and disinfectants Antiseptics and disinfectants.
Optical properties of parietal peritoneum in the spectral range nm Marina D. Kozintseva 1, Alexey N. Bashkatov 1, Elina A. Genina 1, Vyacheslav.
381 PHT Lab #2.
Each microorganism has an optimum growth temperature, which below it growth decreases reversibly and above it decreases irreversibly due to degradation.
OPTICAL CLEARING OF HUMAN SKIN FOR THE ENHANCEMENT OF OPTICAL IMAGING OF PROXIMAL INTERPHALANGEAL JOINTS Ekaterina A. Kolesnikova 1, Aleksandr S.Kolesnikov.
Isolation & Identification of Staphylococci
Staphylococcus Department of pathogenic Biology of Gannan Medical College.
Staphylococcus Gram + cocci In clumps.
Daria K. Tuchina, Alexey N. Bashkatov, Elina A. Genina, Vyacheslav I. Kochubey, Valery V. Tuchin Department of Optics and Biophotonics of Saratov State.
Isolation and Identification of Staphylococci
Classification of Bacteria. There are thousands of species of bacteria on earth, many of which have not yet been identified. When attempting to classify.
DISK ASSAYS Concentration of EXEG 1706 (  g/ml) OD 605nm
The Effect of Three Surface Disinfectants on Four Types of Bacteria Done By: Fatimah Al Bazron Undergraduate Student (Microbiology Concentration) Department.
Introduction In addition to general-purpose media, which allow the growth of most types of bacteria, microbiologists use specialized media to identify.
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology.
Ph. D Abdelraouf A. Elmanama
Kate Bateman Mentors: Dr. Dennis Hruby …… Tove’ Bolken Department of Microbiology.
Medical bacteriology:
Batterjee Medical College. Dr. Manal El Said Head of Microbiology Department Staphylococci Staphylococcus aureus.
Saratov State University ______________________________________________ Department of Optics & Biophotonics __________________________________________________.
Selective and Differential media
Titles Good and Bad Titles. Below you will find an abstract from one of the Bio3B projects. Which of the following title would be most appropriate for.
SELECTIVE, DIFFERENTIAL AND ENRICHED MEDIA
Gram Positive Bacteria and Clinical Case Studies II
Clinical Microbiology ( MLCM- 201) Prof. Dr. Ebtisam.F. El Ghazzawi. Medical Research Institute (MRI) Alexandria University.
Variances seen in Bacterial Analysis for Water and Waste Water Sampling Gretchen Hathaway Whatman Sales Representative July 19, 2007.
Saratov State University ______________________________________________ Department of Optics & Biophotonics __________________________________________________.
Lab 3 Continuation of Stains. Gram Unknown Each student gets one unknown tube of bacteria Gram stain and record –Your name –Unknown number –Gram reaction.
Copyright © 2013, 2010 by Saunders, an imprint of Elsevier Inc. Chapter 83 Basic Principles of Antimicrobial Therapy.
Classification of bacteria
Monitoring of liquids rising in porous medium (paper and dental tissue) using full field speckle-correlation technique Maxim Vilensky 1, Natalia A.Trunina.
Microbial Control Lab 4. Selective and Differential Media We have completed Isolation of bacteria using steak plate and spread plate This is a good beginning,
MICROSCOPES. Microscopes Microscope : an instruments used to examine very small objects (Specimens) in microbiology. Functions : To allow us to study.
Lab 5: INTEGUMENTARY SYSTEM BACTERIOLOGY AND IDENTIFICATION.
Yoga Mats Support the Growth of Staphylococcus aureus Tiffany King, Department of Biological Science, York College of Pennsylvania Introduction  Staphylococcus.
Staphylococcus Dr. Jyotsna Agarwal Professor, Dept. of Microbiology K G Medical University, Lucknow.
University of Tabuk Faculty of Applied Medical Science
Study the effect of industrial antibiotics on microbial growth.
Daria K. Tuchina, 1,2 Rui Shi, 1 Alexey N. Bashkatov, 2 Elina A. Genina, 2 Dan Zhu, 1 Qingming Luo, 1 Valery V. Tuchin Britton Chance Center for.
HONEY ANTIMICROBIAL EFFECTS Alicia Grabiec Freeport Senior High School Grade 10.
Pharmacology Unit 2: Applied Surgical Pharmacology Elsevier items and derived items © 2006 by Saunders, an imprint of Elsevier Inc.
Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case M I C R.
Lab 13 Microbial Control with Chemical Agents. Staphylococcus aureus Escherichia coli Do all disinfectants and antiseptics work equally well against.
Alexey N. Bashkatov, Georgy S. Terentyuk, Elina A. Genina, Daniil A. Chumakov, Artem G. Terentyuk, Vadim D. Genin, Valery V. Tuchin, Saratov State University.
STAPHYLOCOCCI.
STAINING.
Maxim Vilensky, Saratov State University, Russia Oxana V. Semyachkina-Glushkovskaya, Saratov State University, Russia Denis A. Alexandrov, Saratov State.
GK-12 Saturday Workshop December 3, Kirby-Bauer Method The Kirby-Bauer method is the most widely used antibiotic susceptibility test in determining.
Lab #10.
Follicular delivery of nanoparticles and microcontainers ex vivo and in vivo Sergey Zaitsev 1, Natalia Ksenofontova 1, Yulia I. Svenskaya 1, Olga Guslyakova.
Active immunization Immunology and microbiology 2011.
Plants and Fungi Used to Treat Infectious Disease
Dimethyl sulfoxide (DMSO) diffusion in skin tissue Marina D. Kozintseva, Alexey N. Bashkatov, Elina A. Genina, Valery V. Tuchin Department of Optics and.
Selective and differential Media By : Mahmoud W. El-Hindi
Daria K. Tuchina, Alexey N. Bashkatov, Elina A. Genina, Valery V. Tuchin Department of Optics and Biophotonics of Saratov State University, Saratov, Russia.
Inactivation of Mycobacterium smegmatis following Exposure to 405- Nanometer Light OR “Killing Bugs Without Drugs” J. Stephen Guffey – Physical Therapy.
Staphylococcus spp 방소연 자료조사 서유진 자료조사
Antimicrobial Effects of Different Types of Honey against Staphylococcus aureus and Pseudomonas aeruginosa Saad B. Almasaudi, Alaa A. M. Al-Nahari,
Ekaterina N. Lazareva1,2 and Valery V. Tuchin1,2,3
Microbiology: A Systems Approach
Selective and Differential Media
INVESTIGATION OF CHANGE OF TUMOR OPTICAL PROPERTIES AFTER LASER-INDUCED PLASMON-RESONANT PHOTOTHERMAL TREATMENT OF TRANSPLANTED TUMORS IN RATS Vadim.
Introduction In addition to general-purpose media, which allow the growth of most types of bacteria, microbiologists use specialized media to identify.
Antimicrobial Agents and Immunology
Titanium Dioxide Sensitized with Porphyrin Dye as a Photocatalyst for the Degradation of Water Pollutants Kevin Reyes, A.S. & Ivana Jovanovic, Ph.D. Department.
Introduction In addition to general-purpose media, which allow the growth of most types of bacteria, microbiologists use specialized media to identify.
Presentation transcript:

Сombination of Nanoparticles and Photosensitizers in Photodynamic Action on Microorganisms Pavel O. Petrov, Ivan A. Orlov, Alexandr V. Kovalev, Elena S. Tuchina and Valery V. Tuchin Laboratory of Bacteriology Res.-Edu. Institute of Optics and Biophotonics Saratov State University S Saratov S State U University

Goal: To study the sensitivity of staphylococci to the combined effects of light, dyes and nanoparticles

Genera Staphylococcus: Gram-positive cocci arranged in clusters Occurs frequently on the skin and mucous membranes of humans and animals Cause of acne, boils, endocarditis, osteomyelitis, toxic shock syndrome, nosocomial infections, wound infections, etc. Often resistant to a wide variety of antibiotics, including methicillin Contains carotenoids, staphyloxanthin, 4,4'-diaponeurosporene Staphylococcus aureus Staphylococcus epidermidis

S. aureus 209 P S. epidermidis two strains – meticillin-susceptible (MSSE) and meticillin-resistance (MRSE) Staphylococci were grown on nutrient agar and incubated at 37°C Bacteria strains

Experimental combinations Blue and Red LEDs + Methylene Blue + Titanium Dioxide Blue and Red LEDs + Brilliant Green + Titanium Dioxide Infrared laser + Indocyanine Green + Titanium Dioxide For the treatment of infections in the tissues For the treatment of infections on the surface of skin and mucous

Characteristics and parameters Light Exposure 5, 10, 15, 30 min Light Exposure: 5, 10, 15, 30 min Light source Emission maximum, nm Power desity, mW/cm 2 Manufacturer Blue LED 405± Original design, Leonid Dolotov, SSU Red LED 625±2033 IR Laser 805±1550 Aculaser, Photothera Inc., USA Dyes Absorption maximum, nm Concentration Manufacturer Methylene Blue (MB) % Sigma-Aldrich Co., USA Brilliant Green (BG) 440; % Manufacturing pharmaceutical company, Russia Indocyanine green (ICG) % Sigma-Aldrich Co., USA Nanoparticles Titanium Dioxide (TiO 2 ) ; % Sigma-Aldrich Co., USA

Bacterial suspension ( mc/ml) (Fig. 1) Bacterial suspension in PBS (1000 mc/ml) Bacterial suspension in dye water solution (1000 mc/ml) Darkness (control) Darkness (control) Light exposure (Fig. 2) h incubation 37 C on dense nutrient medium Counting the number of colony forming units, using the original digital program (Fig. 3 ) º Fig. 1 Fig. 2 Fig. 3

The action of Blue+Red LEDs, Methylene Blue and TiO 2 on microbial colony forming ability

The action of Blue+Red LEDs, Brilliant Geen and TiO 2 on microbial colony forming ability

The action of IR laser, Indocyanine Green and TiO 2 on microbial colony forming ability

Summary The most effective combination for inhibition the growth of the three investigated staphilococci is the LED light (405, 625 nm), Brilliant Green and TiO 2 nanoparticles. This bacteria killing technology has several advantages: a low power radiation and low concentration of dyes can be used; the effectiveness is achieved through various mechanisms (photodynamic, photocatalytic, photothermal) that prevents the formation of bacterial resistance

Acknowledgments The research described in this paper has been made possible by: Grant PHOTONICS4LIFE of FP7-ICT Grant of President of RF "Supporting of Leading Scientific Schools" - NSHA Projects 2.1.1/4989 and /2950 of RF Program on the Development of High School Potential Project of Federal Agency of Education of RF The Special Program of RF "Scientific and Pedagogical Personnel of Innovative Russia," Governmental contracts: and We are grateful to: Docent Dolotov L.E. (SSU) for the development of the LED light device